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PPM1F (NM_014634) Human Untagged Clone

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SKU
SC114924
PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPM1F
Synonyms CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114924 sequence for NM_014634 edited (data generated by NextGen Sequencing)
ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA
GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG
CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG
CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC
CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG
GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG
GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG
CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC
GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC
CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC
GGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG
CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAGCCTTCCGGCGCACCGACCAG
ATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCAGAGCGGCACCACAGGTGTGTGTGCG
CTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTA
CAGCAGGGACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAGAAG
GCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCACATGGACTGCTGGAGAGTCAACGGG
ACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTTCCAGAAGCCCTACGTGTCTGGGGAG
GCCGATGCAGCTTCCCGGGCGCTGACGGGCTCCGAGGACTACCTGCTGCTTGCCTGTGAT
GGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGTTGGCCTGGTCCAGAGCCACCTGACC
AGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCGGGAGCGG
GGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCCCCAAGAGCTGCTG
GAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAGAAGGGAGGAGGCAGGACTTGCCC
TCCAGCCTTCCAGAACCTGAGACCCAGGCTCCACCAAGAAGCTAG

Clone variation with respect to NM_014634.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014634 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCGCGGAGACCGGG
GGTGAAGCCTGGAGACCCTCTTGCCCTGGCCTAGCTGCAGGCCCCCGGGATGCTTTGGGC
ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA
GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG
CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG
CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC
CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG
GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG
GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG
CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC
GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC
CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC
GGNAGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG
CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAACCTTCCGGCGCACCGACCAG
ATGNNTCTCAGGGAAGCCAAGCGAGAGCGGCTGCAGAGCGCACCACAGTGGTGTGTGCGC
TCATTGGCAGAGCGACCNTGCACGTCGCCTGGCTCGGGGATTCCCAGTCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014634 unedited
CCGCGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCTTGAAAAAAGCTTTAA
AAAAGTAAATACTTAAGTTTTCTAAAGAATAAAATAAATGCTAAGCTCTGCTTAAATTAT
ATGACACAACAGGTCATCTTGGCACTGATAAAACAAAGAGAAAACCTGGAATACTGCTTC
GGAATTAGACCTCCCTGGAAAAAAACAAACACACCAACAAAAGACACATGTGCGTCGCCG
GTTCCGGGCTCAGGGAAGAGAGCAGCCCGGAAGGCCCTGGTGGGCCCAAGCACATCATGG
CTGCCCTGGAGTGGGGGCAGGGGGGTGGCTGCTACAGAACAGACCCCTGGCTCTGCTGCG
CTCTCCTCTTTGGGACACTGGTGTGGCCGACCAGGACTGGCTGGGCATATCTGTAAATAT
GAACCCACCTGGCCCTGGCCGCAGAGAGGAGCAATGGGAAACTGGGGCTGGCCACTCCGC
TGCTGGGCCTGACCCAGGGGGTGCTTCAAGAGTTTGGCTCCTGGGTCCGCCCCAGGGTTC
CACGTGCACAACCATGCTGCCCCATGCACACCAGCGTCTTTGGTTTGGCTGCCGTTGCGC
ACCTATGACCTCTGGACCCACCCCGGGCCTACTAGGACCTGGGAGCAAGAGCCCCTCCAT
CTTTTCTTTGGCGAGGCCTCCTAACCTGCCTTCCCCCTCCTGCCGCCACCAGTGCAGTTC
ACAGCCACAAGAAGGCCTGTGGGGGGTGCTCCAACCGCCTTGGGGCGCTTGGGAAACCCT
GCGGGGCGGGCCCCTGTCCACTGCCTGCACCTGTGGCCCCGAGGCCTCTGAGGAACACGC
CACCGATGCAGCATGTCAGCCCCCCCCCTTCACCTTTTACCCTTGTTTTACCCCCCTTCA
CTATTCAAATCCCTTCCTCCCCTCTTCTATCTCT
Restriction Sites NotI-NotI |
ACCN NM_014634
Insert Size 2440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014634.2, NP_055449.1
RefSeq Size 5169 bp
RefSeq ORF 1365 bp
Locus ID 9647
UniProt ID P49593
Cytogenetics 22q11.22
Domains PP2C
Protein Families Druggable Genome, Phosphatase
Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. provided by RefSeq, Jul 2008
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"NM_014634" in other vectors (7)
SKU Description Size Price
RC210043 PPM1F (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$1,025.00
RC210043L1 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged 10 ug
$1,325.00
RC210043L2 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged 10 ug
$1,325.00
RC210043L3 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged 10 ug
$1,325.00
RC210043L4 Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged 10 ug
$1,325.00
RG210043 PPM1F (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$489.00 $1,225.00
SC320526 PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) 10 ug
$457.00

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