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IFT20 (NM_174887) Human Untagged Clone

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SKU
SC319073
IFT20 (untagged)-Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20)
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IFT20
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_174887.2 GGTAAACAGGCTCTGTATCCGTGGCAGCGGCCGTGGCAGGCTGGCTGGGTACCGGCTGTC
GCTGACCCAGGAGAAGCTGCCTGTCTACATCAGCCTGGGCTGCAGCGCGCTGCCGCCGCG
GGGCCGGCAGCCATGGCCAAGGACATCCTGGGTGAAGCAGGGCTACACTTTGATGAACTG
AACAAGCTGAGGGTGTTGGACCCAGAGGTTACCCAGCAGACCATAGAGCTGAAGGAAGAG
TGCAAAGACTTTGTGGACAAAATTGGCCAGTTTCAGAAAATAGTTGGTGGTTTAATTGAG
CTTGTTGATCAACTTGCAAAAGAAGCAGAAAATGAAAAGATGAAGAGTCTTGCTGTGTCA
CCCAGGCTGGAGTGCACTGGTGCAATCTCGGCTCACTGCAAGCTCTGCCTCTCAGATTCA
AGCGATTCTCCCACCTCACCCTCCCGAGTAGGTGGGACTACAGGCCATCGGTGCTCGGAA
CTTGCTCAAATCTATAGCAAAGCAGAGAGAAGCTCAACAGCAGCAACTTCAAGCCCTAAT
AGCAGAAAAGAAAATGCAGCTAGAAAGGTATCGGGTTGAATATGAAGCTTTGTGTAAAGT
AGAAGCAGAACAAAATGAATTTATTGACCAATTTATTTTTCAGAAATGAACTGAAAATTT
CGCTTTTATAGTAGGAAGGCAAAACAAAAAAAAGCCTCTCAAAACCAAAAAAACCTCTGT
AGCATTCCAGCGGCTTGACCAATGACCTATGTCACAAGAGGTGGCGTGTAAGGAATGCAG
CCCCCTGAAGACAGCACTACAAGTCTGGGGGAGCCAGTTTTAACATCAGTGCACAGCTGC
TGCTGGTGGCCCTGCAGTGTACGTTCTCACCTCTTATGCTTAGTTGGAACTAAGCAGTTT
GTAAACTTTCATCCTTTTTTTTGTAAATTCACAAAGCTTTGGAAGGAGAAGCAATAAATT
TTTGTTTTCAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_174887
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174887.2, NP_777547.1
RefSeq Size 1016 bp
RefSeq ORF 447 bp
Locus ID 90410
UniProt ID Q8IY31
Cytogenetics 17q11.2
Summary This gene encodes a intraflagellar transport protein important for intracellular transport. The encoded protein forms part of a complex involved in trafficking of proteins from the Golgi body, including recycling of immune signalling components (Finetti et al., PubMed: 19855387). This gene is part of a complex set of sense-antisense loci that may be co-regulated. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 14.provided by RefSeq, Jun 2012
Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR, cause translation initiation at a downstream start codon, and result in a frameshift compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1 and has a distinct C-terminus.
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"NM_174887" in other vectors (6)
SKU Description Size Price
RC201337 IFT20 (Myc-DDK-tagged)-Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20) 10 ug
$150.00
RC201337L1 Lenti ORF clone of Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20), Myc-DDK-tagged 10 ug
$450.00
RC201337L2 Lenti ORF clone of Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20), mGFP tagged 10 ug
$450.00
RC201337L3 Lenti ORF clone of Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20), Myc-DDK-tagged 10 ug
$450.00
RC201337L4 Lenti ORF clone of Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20), mGFP tagged 10 ug
$450.00
RG201337 IFT20 (tGFP-tagged) - Human intraflagellar transport 20 homolog (Chlamydomonas) (IFT20) 10 ug
$489.00

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