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WHSC2 (NELFA) (NM_005663) Human Untagged Clone

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SKU
SC317896
NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2)
  $553.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WHSC2
Synonyms NELF-A; P/OKcl.15; WHSC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317896 representing NM_005663.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGGGGCAGCGGCGCGCGCTTTCCCCCAAGATGGCGTCCATGCGGGAGAGCGACACGGGCCTGTGG
CTGCACAACAAGCTGGGGGCCACGGACGAGCTGTGGGCGCCGCCCAGCATCGCGTCCCTGCTCACGGCC
GCGGTCATCGACAACATCCGTCTCTGCTTCCATGGCCTCTCGTCGGCAGTGAAGCTCAAGTTGCTACTC
GGGACGCTGCACCTCCCGCGCCGCACGGTGGACGAGATGAAGGGCGCCCTAATGGAGATCATCCAGCTC
GCCAGCCTCGACTCGGACCCCTGGGTGCTCATGGTCGCCGACATCTTGAAGTCCTTTCCGGACACAGGC
TCGCTTAACCTGGAGCTGGAGGAGCAGAATCCCAACGTTCAGGATATTTTGGGAGAACTTAGAGAAAAG
GTGGGTGAGTGTGAAGCGTCTGCCATGCTGCCACTGGAGTGCCAGTACTTGAACAAAAACGCCCTGACG
ACCCTCGCGGGACCCCTCACTCCCCCGGTGAAGCATTTTCAGTTAAAGCGGAAACCCAAGAGCGCCACG
CTGCGGGCGGAGCTGCTGCAGAAGTCCACGGAGACCGCCCAGCAGTTGAAGCGGAGCGCCGGGGTGCCC
TTCCACGCCAAGGGCCGGGGGCTGCTGCGGAAGATGGACACCACCACCCCACTCAAAGGCATCCCGAAG
CAGGCGCCCTTCAGAAGCCCCACGGCGCCCAGCGTCTTCAGCCCCACAGGGAACCGGACCCCCATCCCG
CCTTCCAGGACGCTGCTGCGGAAGGAACGAGGTGTGAAGCTGCTGGACATCTCTGAGCTGGATATGGTT
GGCGCTGGCCGAGAGGCGAAGCGGAGAAGGAAGACTCTCGATGCGGAGGTGGTGGAGAAGCCGGCCAAG
GAGGAAACGGTGGTGGAGAACGCCACCCCGGACTACGCAGCCGGCCTGGTGTCCACGCAGAAACTTGGG
TCCCTGAACAATGAGCCTGCGCTGCCCTCCACGAGCTACCTTCCCTCCACGCCCAGCGTGGTTCCCGCC
TCCTCCTACATCCCCAGCTCCGAGACGCCCCCAGCCCCATCTTCCCGGGAAGCCAGCCGCCCACCAGAG
GAGCCCAGCGCCCCGAGCCCCACGTTGCCAGCGCAGTTCAAGCAGCGGGCGCCCATGTACAACAGCGGC
CTGAGCCCTGCCACACCCACGCCTGCGGCGCCCACCTCGCCTCTGACACCCACCACACCTCCGGCTGTC
GCCCCTACCACTCAGACACCCCCGGTTGCCATGGTGGCCCCGCAGACCCAGGCCCCTGCTCAGCAGCAG
CCTAAGAAGAACCTGTCCCTCACGAGAGAGCAGATGTTCGCTGCCCAGGAGATGTTCAAGACGGCCAAC
AAAGTCACGCGGCCCGAGAAGGCCCTCATCCTGGGCTTCATGGCCGGCTCCCGAGAGAACCCGTGCCAG
GAGCAGGGGGACGTGATCCAGATCAAGCTGAGCGAGCACACGGAGGACCTGCCCAAGGCGGACGGCCAG
GGTAGCACAACCATGCTGGTGGACACAGTGTTTGAGATGAACTATGCCACGGGCCAGTGGACGCGCTTC
AAGAAGTACAAGCCCATGACCAATGTGTCCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_005663
Insert Size 1620 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005663.4
RefSeq Size 2479 bp
RefSeq ORF 1620 bp
Locus ID 7469
UniProt ID Q9H3P2
Cytogenetics 4p16.3
Protein Families Transcription Factors
MW 58.5 kDa
Summary This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005663" in other vectors (8)
SKU Description Size Price
RC201097 NELFA (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$492.00
RC201097L1 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), Myc-DDK-tagged 10 ug
$792.00
RC201097L2 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), mGFP tagged 10 ug
$792.00
RC201097L3 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), Myc-DDK-tagged 10 ug
$792.00
RC201097L4 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), mGFP tagged 10 ug
$792.00
RG201097 NELFA (tGFP-tagged) - Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$489.00 $692.00
SC319082 NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$492.00
SC327912 NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$553.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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