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WHSC2 (NELFA) (NM_005663) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201097
NELFA (tGFP-tagged) - Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $692.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WHSC2
Synonyms NELF-A; P/OKcl.15; WHSC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201097 representing NM_005663
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCATGCGGGAGAGCGACACGGGCCTGTGGCTGCACAACAAGCTGGGGGCCACGGACGAGCTGT
GGGCGCCGCCCAGCATCGCGTCCCTGCTCACGGCCGCGGTCATCGACAACATCCGTCTCTGCTTCCATGG
CCTCTCGTCGGCAGTGAAGCTCAAGTTGCTACTCGGGACGCTGCACCTCCCGCGCCGCACGGTGGACGAG
ATGAAGGGCGCCCTAATGGAGATCATCCAGCTCGCCAGCCTCGACTCGGACCCCTGGGTGCTCATGGTCG
CCGACATCTTGAAGTCCTTTCCGGACACAGGCTCGCTTAACCTGGAGCTGGAGGAGCAGAATCCCAACGT
TCAGGATATTTTGGGAGAACTTAGAGAAAAGGTGGGTGAGTGTGAAGCGTCTGCCATGCTGCCACTGGAG
TGCCAGTACTTGAACAAAAACGCCCTGACGACCCTCGCGGGACCCCTCACTCCCCCGGTGAAGCATTTTC
AGTTAAAGCGGAAACCCAAGAGCGCCACGCTGCGGGCGGAGCTGCTGCAGAAGTCCACGGAGACCGCCCA
GCAGTTGAAGCGGAGCGCCGGGGTGCCCTTCCACGCCAAGGGCCGGGGGCTGCTGCGGAAGATGGACACC
ACCACCCCACTCAAAGGCATCCCGAAGCAGGCGCCCTTCAGAAGCCCCACGGCGCCCAGCGTCTTCAGCC
CCACAGGGAACCGGACCCCCATCCCGCCTTCCAGGACGCTGCTGCGGAAGGAACGAGGTGTGAAGCTGCT
GGACATCTCTGAGCTGGATATGGTTGGCGCTGGCCGAGAGGCGAAGCGGAGAAGGAAGACTCTCGATGCG
GAGGTGGTGGAGAAGCCGGCCAAGGAGGAAACGGTGGTGGAGAACGCCACCCCGGACTACGCAGCCGGCC
TGGTGTCCACGCAGAAACTTGGGTCCCTGAACAATGAGCCTGCGCTGCCCTCCACGAGCTACCTTCCCTC
CACGCCCAGCGTGGTTCCCGCCTCCTCCTACATCCCCAGCTCCGAGACACCCCCAGCCCCATCTTCCCGG
GAAGCCAGCCGCCCACCAGAGGAGCCCAGCGCCCCGAGCCCCACGTTGCCAGCGCAGTTCAAGCAGCGGG
CGCCCATGTACAACAGCGGCCTGAGCCCTGCCACACCCACGCCTGCGGCGCCCACCTCGCCTCTGACACC
CACCACACCTCCGGCTGTCGCCCCTACCACTCAGACACCCCCGGTTGCCATGGTGGCCCCGCAGACCCAG
GCCCCTGCTCAGCAGCAGCCTAAGAAGAACCTGTCCCTCACGAGAGAGCAGATGTTCGCTGCCCAGGAGA
TGTTCAAGACGGCCAACAAAGTCACGCGGCCCGAGAAGGCCCTCATCCTGGGCTTCATGGCCGGCTCCCG
AGAGAACCCGTGCCAGGAGCAGGGGGACGTGATCCAGATCAAGCTGAGCGAGCACACGGAGGACCTGCCC
AAGGCGGACGGCCAGGGTAGCACAACCATGCTGGTGGACACAGTGTTTGAGATGAACTATGCCACGGGCC
AGTGGACGCGCTTCAAGAAGTACAAGCCCATGACCAATGTGTCC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201097 representing NM_005663
Red=Cloning site Green=Tags(s)

MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDE
MKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLE
CQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDT
TTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDA
EVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSR
EASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQ
APAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLP
KADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_005663
ORF Size 1584 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005663.2, NP_005654.2
RefSeq Size 2263 bp
RefSeq ORF 1587 bp
Locus ID 7469
UniProt ID Q9H3P2
Cytogenetics 4p16.3
Protein Families Transcription Factors
Summary This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005663" in other vectors (8)
SKU Description Size Price
RC201097 NELFA (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$492.00
RC201097L1 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), Myc-DDK-tagged 10 ug
$792.00
RC201097L2 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), mGFP tagged 10 ug
$792.00
RC201097L3 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), Myc-DDK-tagged 10 ug
$792.00
RC201097L4 Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2), mGFP tagged 10 ug
$792.00
SC317896 NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$553.00
SC319082 NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$492.00
SC327912 NELFA (untagged)-Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2) 10 ug
$553.00

Plasmid Map for RG201097

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201097
Circular map for RG201097

file-alt Citations

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