PHF23 (NM_024297) Human Untagged Clone

SKU
SC317643
PHF23 (untagged)-Human PHD finger protein 23 (PHF23)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHF23
Synonyms hJUNE-1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317643 representing NM_024297.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGGAAGCCATGGCGGAGCCCAGTCCCGAAGATCCACCTCCGACCCTTAAGCCAGAGACTCAGCCA
CCAGAGAAACGGCGGAGAACAATTGAGGATTTCAACAAATTCTGCAGTTTTGTTTTGGCATATGCTGGT
TACATTCCCCCTAGCAAAGAGGAAAGTGACTGGCCAGCCTCTGGCTCCAGCTCTCCATTGCGAGGAGAG
AGTGCGGCCGACAGTGATGGCTGGGACTCGGCCCCCTCAGATCTTCGAACCATCCAGACTTTTGTTAAG
AAAGCAAAGTCATCCAAGAGAAGGGCAGCTCAAGCAGGTCCCACCCAGCCAGGACCCCCAAGGTCCACT
TTCTCTCGTCTGCAGGCCCCCGACAGTGCTACCTTGCTTGAGAAGATGAAGCTCAAGGACTCTCTCTTT
GATCTGGATGGGCCCAAAGTGGCATCTCCTTTGTCCCCCACATCCCTGACACATACCTCCCGGCCCCCT
GCTGCTCTTACCCCCGTGCCCCTTTCCCAGGGGGACCTCTCCCATCCTCCTCGAAAGAAGGACCGAAAG
AACCGAAAGTTGGGGCCAGGAGCTGGGGCTGGCTTTGGGGTGCTTCGGAGGCCTCGGCCAACTCCTGGG
GATGGGGAAAAGAGATCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTAAAAAAGGCAGAACGGGGGGAT
AGACTCCCACCTCCTGGGCCTCCCCAGGCACCCCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAG
GAGGAAGAGGAAGAAGAAGAAGAGATGGCAACAGTGGTAGGGGGTGAAGCCCCAGTCCCTGTGCTGCCA
ACACCCCCTGAGGCTCCTAGGCCCCCTGCCACAGTGCACCCTGAAGGAGTCCCTCCTGCTGACAGTGAA
AGCAAGGAGGTGGGCAGCACTGAAACAAGCCAAGATGGAGATGCCAGCTCCAGTGAAGGCGAGATGCGG
GTCATGGACGAGGACATCATGGTAGAATCAGGTGATGACTCATGGGATCTGATCACATGTTACTGTCGA
AAGCCCTTTGCAGGGCGGCCCATGATTGAGTGCAGCCTGTGTGGGACGTGGATCCACCTCTCCTGTGCT
AAGATTAAGAAGACCAACGTCCCCGACTTCTTTTATTGCCAGAAATGCAAGGAACTGAGGCCAGAGGCC
CGGCGGTTAGGGGGGCCTCCCAAATCTGGAGAGCCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_024297
Insert Size 1212 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024297.2
RefSeq Size 2041 bp
RefSeq ORF 1212 bp
Locus ID 79142
UniProt ID Q9BUL5
Cytogenetics 17p13.1
Protein Families Druggable Genome, Transcription Factors
MW 43.8 kDa
Summary Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_024297" in other vectors (7)
SKU Description Size Price
RC200931 PHF23 (Myc-DDK-tagged)-Human PHD finger protein 23 (PHF23) 10 ug
$457.00
RC200931L1 Lenti ORF clone of Human PHD finger protein 23 (PHF23), Myc-DDK-tagged 10 ug
$757.00
RC200931L2 Lenti ORF clone of Human PHD finger protein 23 (PHF23), mGFP tagged 10 ug
$757.00
RC200931L3 Lenti ORF clone of Human PHD finger protein 23 (PHF23), Myc-DDK-tagged 10 ug
$757.00
RC200931L4 Lenti ORF clone of Human PHD finger protein 23 (PHF23), mGFP tagged 10 ug
$757.00
RG200931 PHF23 (tGFP-tagged) - Human PHD finger protein 23 (PHF23) 10 ug
$489.00 $657.00
SC122955 PHF23 (untagged)-Human PHD finger protein 23 (PHF23) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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