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PHF23 (NM_024297) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200931
PHF23 (tGFP-tagged) - Human PHD finger protein 23 (PHF23)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHF23
Synonyms hJUNE-1b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200931 representing NM_024297
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGAAGCCATGGCGGAGCCCAGTCCCGAAGATCCACCTCCGACCCTTAAGCCAGAGACTCAGCCAC
CAGAGAAACGGCGGAGAACAATTGAGGATTTCAACAAATTCTGCAGTTTTGTTTTGGCATATGCTGGTTA
CATTCCCCCTAGCAAAGAGGAAAGTGACTGGCCAGCCTCTGGCTCCAGCTCTCCATTGCGAGGAGAGAGT
GCGGCCGACAGTGATGGCTGGGACTCGGCCCCCTCAGATCTTCGAACCATCCAGACTTTTGTTAAGAAAG
CAAAGTCATCCAAGAGAAGGGCAGCTCAAGCAGGTCCCACCCAGCCAGGACCCCCAAGGTCCACTTTCTC
TCGTCTGCAGGCCCCCGACAGTGCTACCTTGCTTGAGAAGATGAAGCTCAAGGACTCTCTCTTTGATCTG
GATGGGCCCAAAGTGGCATCTCCTTTGTCCCCCACATCCCTGACACATACCTCCCGGCCCCCTGCTGCTC
TTACCCCCGTGCCCCTTTCCCAGGGGGACCTCTCCCATCCTCCTCGAAAGAAGGACCGAAAGAACCGAAA
GTTGGGGCCAGGAGCTGGGGCTGGCTTTGGGGTGCTTCGGAGGCCTCGGCCAACTCCTGGGGATGGGGAA
AAGAGATCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTAAAAAAGGCAGAACGGGGGGATAGACTCCCAC
CTCCTGGGCCTCCCCAGGCACCCCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAGGAAGAGGA
AGAAGAAGAAGAGATGGCAACAGTGGTAGGGGGTGAAGCCCCAGTCCCTGTGCTGCCAACACCCCCTGAG
GCTCCTAGGCCCCCTGCCACAGTGCACCCTGAAGGAGTCCCTCCTGCTGACAGTGAAAGCAAGGAGGTGG
GCAGCACTGAAACAAGCCAAGATGGAGATGCCAGCTCCAGTGAAGGCGAGATGCGGGTCATGGACGAGGA
CATCATGGTAGAATCAGGTGATGACTCATGGGATCTGATCACATGTTACTGTCGAAAGCCCTTTGCAGGG
CGGCCCATGATTGAGTGCAGCCTGTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAGACCA
ACGTCCCCGACTTCTTTTATTGCCAGAAATGCAAGGAACTGAGGCCAGAGGCCCGGCGGTTAGGGGGGCC
TCCCAAATCTGGAGAGCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200931 representing NM_024297
Red=Cloning site Green=Tags(s)

MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPASGSSSPLRGES
AADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFSRLQAPDSATLLEKMKLKDSLFDL
DGPKVASPLSPTSLTHTSRPPAALTPVPLSQGDLSHPPRKKDRKNRKLGPGAGAGFGVLRRPRPTPGDGE
KRSRIKKSKKRKLKKAERGDRLPPPGPPQAPPSDTDSEEEEEEEEEEEEEEMATVVGGEAPVPVLPTPPE
APRPPATVHPEGVPPADSESKEVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAG
RPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKSGEP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024297
ORF Size 1209 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024297.3
RefSeq Size 2041 bp
RefSeq ORF 1212 bp
Locus ID 79142
UniProt ID Q9BUL5
Cytogenetics 17p13.1
Protein Families Druggable Genome, Transcription Factors
Summary Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.UniProtKB/Swiss-Prot Function
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"NM_024297" in other vectors (7)
SKU Description Size Price
RC200931 PHF23 (Myc-DDK-tagged)-Human PHD finger protein 23 (PHF23) 10 ug
$457.00
RC200931L1 Lenti ORF clone of Human PHD finger protein 23 (PHF23), Myc-DDK-tagged 10 ug
$757.00
RC200931L2 Lenti ORF clone of Human PHD finger protein 23 (PHF23), mGFP tagged 10 ug
$757.00
RC200931L3 Lenti ORF clone of Human PHD finger protein 23 (PHF23), Myc-DDK-tagged 10 ug
$757.00
RC200931L4 Lenti ORF clone of Human PHD finger protein 23 (PHF23), mGFP tagged 10 ug
$757.00
SC122955 PHF23 (untagged)-Human PHD finger protein 23 (PHF23) 10 ug
$300.00
SC317643 PHF23 (untagged)-Human PHD finger protein 23 (PHF23) 10 ug
$503.00

Plasmid Map for RG200931

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200931
Circular map for RG200931

file-alt Citations

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