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SCD5 (NM_024906) Human Untagged Clone

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SKU
SC312669
SCD5 (untagged)-Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 2
  $330.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SCD5
Synonyms ACOD4; FADS4; HSCD5; SCD2; SCD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_024906
Insert Size 771 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024906.2
RefSeq Size 2080 bp
RefSeq ORF 771 bp
Locus ID 79966
UniProt ID Q86SK9
Cytogenetics 4q21.22
Domains FA_desaturase
Protein Families Transmembrane
Protein Pathways Biosynthesis of unsaturated fatty acids, PPAR signaling pathway
MW 29 kDa
Summary Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 (MIM 604031) and SCD5, have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al., 2005 PubMed 15907797).supplied by OMIM, Mar 2008
Transcript Variant: This variant (2) has multiple differences in the 3' coding region, compared to variant 1, which result in a protein (isoform B) with a shorter and distinct C-terminus, compared to isoform A.
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"NM_024906" in other vectors (4)
SKU Description Size Price
RC216929 SCD5 (Myc-DDK-tagged)-Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 2 10 ug
$300.00
RC216929L3 Lenti ORF clone of Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC216929L4 Lenti ORF clone of Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 2, mGFP tagged 10 ug
$600.00
RG216929 SCD5 (tGFP-tagged) - Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 2 10 ug
$500.00

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