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MAP4K1 (NM_007181) Human Untagged Clone

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SKU
SC310086
MAP4K1 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2
  $840.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAP4K1
Synonyms HPK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC310086 representing NM_007181.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTG
GGTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAG
ATGGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGC
CGGCACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAA
TTCTGTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGC
TATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATC
AAGGGAGCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAG
ATTGGGGCTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCA
GCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAA
CTGGCCGAGCTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGT
GGCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTC
ACTCTGACTAAGAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAG
CCTGGGCTGAATCGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCC
ATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCAC
CGCTCCAGCTCTCTGGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGA
GGAATGGAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGC
CCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAG
GACACACCTCCTCCACTTCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATG
GGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCC
CGTCCTGGGCCTCCCCCATCCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAAC
CCACCCTCCCGGGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGA
TGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACAT
CCCTCCACCAAGGACCAGCACCTGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGAC
CAGGAGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTC
ATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAG
ACCAGAGCAGGAAACCCCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCC
ACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCG
TTCCTGTGCGGTGCATTGGAGACGTCCGTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTG
CTTGTCCGGCAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGC
TCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACG
GTGCGCTTTGGCGCGCTCTCTTGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTG
ACCCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGG
TCCCCAGTCCGGGGACTTCGCACACCTGAGATCCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTT
GGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTA
CAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACG
ATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCT
GGGATTACAGGCCTGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_007181
Insert Size 2502 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007181.5
RefSeq Size 2819 bp
RefSeq ORF 2502 bp
Locus ID 11184
UniProt ID Q92918
Cytogenetics 19q13.2
Domains CNH, pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
MW 91.3 kDa
Summary Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2).
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Documents
Resources
"NM_007181" in other vectors (7)
SKU Description Size Price
RC220698 MAP4K1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$763.00
RC220698L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC220698L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged 10 ug
$1,063.00
RC220698L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC220698L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged 10 ug
$1,063.00
RG220698 MAP4K1 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$963.00
SC323528 MAP4K1 (untagged)-Kinase deficient mutant (K46M) of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$764.00

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