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MAP4K1 (NM_007181) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG220698
MAP4K1 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $963.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP4K1
Synonyms HPK1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG220698 representing NM_007181
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG
GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGAT
GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGG
CACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCT
GTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGT
CTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGA
GCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGG
CTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGC
CCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAG
CTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGC
CTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAA
GAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAAT
CGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTG
AGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCT
GGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGA
CCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGT
CAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACT
TCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTG
AGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCAT
CCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGA
CAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTG
TTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACC
TGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCT
CTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCC
CACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTC
ACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTG
CCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCC
GTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGC
CGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGT
GAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTG
GGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGT
TGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGAT
CCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGA
GTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTC
TGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTC
AAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG220698 representing NM_007181
Red=Cloning site Green=Tags(s)

MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCR
HANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKG
ANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAE
LQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLN
RGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETR
PPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQL
SPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKL
FNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTP
HLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETS
VVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWL
GEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHG
VQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007181
ORF Size 2499 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007181.6
RefSeq Size 2732 bp
RefSeq ORF 2502 bp
Locus ID 11184
UniProt ID Q92918
Cytogenetics 19q13.2
Domains CNH, pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
Summary Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007181" in other vectors (7)
SKU Description Size Price
RC220698 MAP4K1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$763.00
RC220698L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC220698L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged 10 ug
$1,063.00
RC220698L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC220698L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged 10 ug
$1,063.00
SC310086 MAP4K1 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$840.00
SC323528 MAP4K1 (untagged)-Kinase deficient mutant (K46M) of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 10 ug
$764.00

Plasmid Map for RG220698

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG220698
Circular map for RG220698

file-alt Citations

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