ARID5B (NM_032199) Human Untagged Clone

SKU SC305411

ARID5B (untagged)-Human AT rich interactive domain 5B (MRF1-like) (ARID5B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARID5B
• Synonyms: DESRT; MRF-2; MRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305411 representing NM_032199.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGCCCAACTCACTCCAGTGGGTCGGCTCACCGTGTGGCTTGCACGGACCTTACATTTTCTACAAG; GCTTTTCAATTCCACCTTGAAGGCAAACCAAGAATTTTGTCCCTTGGCGACTTTTTCTTTGTAAGATGT; ACGCCAAAGGATCCGATTTGCATAGCGGAGCTCCAGCTGTTGTGGGAAGAGAGGACCAGCCGGCAACTT; TTATCCAGCTCTAAACTTTATTTCCTCCCAGAAGACACTCCCCAGGGCAGAAATAGCGACCATGGCGAG; GATGAAGTCATTGCTGTTTCCGAAAAGGTGATTGTGAAGCTTGAAGACCTGGTCAAGTGGGTACATTCT; GATTTCTCCAAGTGGAGATGTGGCTTCCACGCTGGACCAGTGAAAACTGAGGCCTTGGGAAGGAATGGA; CAGAAGGAAGCTCTGCTGAAGTACAGGCAGTCAACCCTAAACAGTGGACTCAACTTCAAAGACGTTCTC; AAGGAGAAGGCAGACCTGGGGGAGGACGAGGAAGAAACGAACGTGATAGTTCTCAGCTACCCCCAGTAC; TGCCGGTACCGCTCGATGCTGAAACGCATCCAGGATAAGCCATCTTCCATTCTAACGGACCAGTTTGCA; TTGGCCCTGGGGGGCATTGCAGTGGTCAGCAGGAACCCTCAGATCCTGTACTGTCGGGACACCTTTGAC; CACCCGACTCTCATAGAAAACGAGAGTATATGCGATGAGTTTGCGCCAAATCTTAAAGGCAGACCACGC; AAAAAGAAACCATGCCCACAAAGAAGAGATTCATTCAGTGGTGTTAAGGATTCCAACAACAATTCCGAT; GGCAAAGCCGTTGCCAAGGTGAAATGTGAGGCCAGGTCAGCCTTGACCAAGCCGAAGAATAACCATAAC; TGTAAAAAAGTCTCAAATGAAGAAAAACCAAAGGTTGCCATTGGTGAAGAGTGCAGGGCAGATGAACAA; GCCTTCTTGGTGGCACTTTATAAATACATGAAAGAAAGGAAAACGCCGATAGAACGAATACCCTATTTA; GGTTTTAAACAGATTAACCTTTGGACTATGTTTCAAGCTGCTCAAAAACTGGGAGGATATGAAACAATA; ACAGCCCGCCGTCAGTGGAAACATATTTATGATGAATTAGGCGGTAATCCTGGGAGCACCAGCGCTGCC; ACTTGTACCCGCAGACATTATGAAAGATTAATCCTACCATATGAAAGATTTATTAAAGGAGAAGAAGAT; AAGCCCCTGCCTCCAATCAAACCTCGGAAACAGGAGAACAGTTCACAGGAAAATGAGAACAAAACAAAA; GTATCTGGAACCAAACGCATCAAACATGAAATACCTAAAAGCAAGAAAGAAAAAGAAAATGCCCCAAAG; CCCCAGGATGCAGCAGAGGTTTCATCAGAGCAAGAAAAAGAACAAGAGACTTTAATAAGCCAGAAAAGC; ATCCCTGAGCCTCTCCCAGCAGCAGACATGAAGAAAAAAATAGAAGGGTATCAGGAATTTTCAGCGAAG; CCCCTGGCATCCAGAGTAGACCCAGAGAAGGACAACGAAACAGACCAAGGTTCCAACAGTGAGAAGGTG; GCAGAGGAGGCGGGAGAGAAGGGGCCCACACCTCCACTCCCAAGTGCTCCTCTGGCCCCAGAAAAAGAT; TCAGCCTTGGTCCCTGGGGCCAGCAAACAGCCACTCACCTCTCCTAGTGCCCTGGTGGACTCAAAACAA; GAATCCAAACTGTGCTGTTTTACAGAGAGCCCTGAAAGTGAACCCCAAGAAGCATCCTTCCCCAGCTTC; CCCACCACACAGCCACCGCTGGCAAACCAGAATGAGACGGAGGATGACAAACTGCCCGCCATGGCAGAT; TACATTGCCAACTGCACCGTGAAGGTGGACCAGCTGGGCAGTGACGACATCCACAATGCGCTCAAGCAG; ACCCCAAAGGTCCTTGTGGTCCAGTCGTTTGACATGTTCAAAGACAAAGACCTGACTGGGCCCATGAAC; GAGAACCATGGACTTAATTACACGCCCCTGCTCTACTCTAGGGGCAACCCAGGCATCATGTCCCCACTG; GCCAAGAAAAAGCTTTTGTCCCAAGTGAGTGGGGCCAGCCTCTCCAGCAGCTACCCTTATGGCTCCCCA; CCCCCTTTGATCAGCAAAAAGAAACTGATTGCTAGGGATGACTTGTGTTCCAGTTTGTCCCAGACCCAC; CATGGCCAAAGCACTGACCATATGGCGGTCAGCCGGCCATCAGTGATTCAGCACGTCCAGAGTTTCAGA; AGCAAGCCCTCGGAAGAGAGAAAGACCATCAATGACATCTTTAAGCATGAGAAACTGAGTCGATCAGAT; CCCCACCGCTGCAGCTTCTCCAAGCATCACCTTAACCCCCTTGCTGACTCCTACGTCCTGAAGCAAGAA; ATTCAGGAGGGCAAGGATAAACTCTTAGAGAAAAGGGCCCTCCCCCATTCCCACATGCCTAGCTTCCTG; GCTGACTTCTACTCGTCCCCTCATCTCCATAGCCTCTACAGACACACCGAGCACCATCTTCATAATGAA; CAGACATCCAAATACCCTTCCAGGGACATGTACAGGGAATCGGAAAACAGTTCTTTTCCTTCCCACAGA; CACCAAGAAAAGCTCCATGTAAATTATCTCACGTCCCTGCACCTGCAAGACAAAAAGTCGGCGGCAGCA; GAAGCCCCTACGGATGATCAGCCTACAGATCTGAGCCTTCCCAAGAACCCGCACAAACCTACCGGCAAG; GTCCTGGGCCTGGCTCATTCCACCACAGGGCCCCAGGAGAGCAAAGGCATCTCCCAGTTCCAGGTCTTA; GGCAGCCAGAGTCGAGACTGTCACCCCAAAGCCTGTCGGGTATCACCCATGACCATGTCAGGCCCTAAA; AAATACCCTGAATCGCTTTCAAGATCAGGAAAACCTCACCATGTGAGACTGGAGAATTTCAGGAAGATG; GAAGGCATGGTCCACCCAATCCTGCACCGGAAAATGAGCCCGCAGAACATTGGGGCGGCGCGGCCGATC; AAGCGCAGCCTGGAGGATTTGGACCTTGTGATTGCAGGGAAAAAGGCCCGGGCAGTGTCTCCCTTAGAC; CCATCCAAGGAGGTCTCTGGGAAGGAGAAGGCCTCTGAGCAGGAGAGTGAAGGCAGCAAAGCAGCGCAC; GGTGGGCATTCCGGGGGCGGATCAGAAGGCCACAAGCTTCCCCTCTCCTCCCCTATCTTCCCAGGTCTG; TATTCCGGGAGCCTGTGTAACTCGGGCCTCAACTCCAGGCTCCCGGCTGGGTATTCTCATTCTCTGCAG; TACTTGAAAAACCAGACTGTGCTTTCTCCACTCATGCAGCCCCTGGCTTTCCACTCGCTTGTGATGCAA; AGAGGAATTTTTACATCACCGACAAATTCTCAGCAGCTGTACAGACACTTGGCTGCGGCTACACCTGTA; GGAAGTTCATATGGGGACCTTTTGCATAACAGCATTTACCCTTTAGCTGCTATAAATCCTCAAGCTGCC; TTTCCATCTTCCCAGCTGTCATCCGTGCACCCCAGTACAAAACTGTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_032199
• Insert Size: 3567 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032199.2
• RefSeq Size: 7948 bp
• RefSeq ORF: 3567 bp
• Locus ID: 84159
• UniProt ID: Q14865
• Cytogenetics: 10q21.2
• MW: 132.4 kDa
• Summary: This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.