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FISH (SH3PXD2A) (NM_014631) Human Untagged Clone

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SKU
SC304145
SH3PXD2A (untagged)-Human SH3 and PX domains 2A (SH3PXD2A)
  $1,540.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FISH
Synonyms FISH; SH3MD1; TKS5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304145 representing NM_014631.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTCGCCTACTGCGTGCAGGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAACCCCTCCAAGCAC
TACGTATACATAATCAATGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAG
TTCTTTGACCTGCAGATGCAGCTTTTGGATAAGTTTCCCATTGAAGGTGGCCAGAAGGACCCCAAGCAA
AGGATCATCCCCTTCCTCCCAGGCAAGATCCTCTTCCGCAGAAGCCACATCCGGGACGTAGCTGTGAAG
AGACTGAAGCCCATCGATGAATACTGCCGGGCACTTGTCCGGCTGCCCCCCCACATCTCACAGTGTGAC
GAAGTCTTCCGGTTCTTCGAGGCTCGACCCGAGGATGTCAACCCTCCAAAAGAGGACTATGGCAGTTCC
AAGAGGAAATCAGTGTGGCTGTCCAGCTGGGCTGAGTCGCCCAAGAAGGACGTGACAGGTGCCGACGCC
ACCGCCGAGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAGCAGGAGAACTCGGAG
CTGAGCCTCCAGGCCGGGGAGGTGGTGGATGTCATCGAGAAGAACGAGAGCGGCTGGTGGTTCGTGAGC
ACTTCTGAGGAGCAGGGCTGGGTCCCTGCCACCTACCTGGAGGCCCAGAATGGTACTCGGGATGACTCC
GACATCAACACCTCTAAGACTGGAGAAGAGGAGAAGTATGTCACCGTGCAGCCTTACACCAGCCAAAGC
AAGGACGAGATTGGCTTTGAGAAGGGCGTCACAGTGGAGGTGATCCGGAAGAATCTGGAAGGCTGGTGG
TATATCAGATACCTGGGCAAAGAGGGCTGGGCGCCAGCATCCTACCTGAAGAAGGCCAAGGATGACCTG
CCAACCCGGAAGAAGAACCTGGCCGGCCCAGTGGAGATCATTGGGAACATCATGGAGATCAGCAACCTG
CTGAACAAGAAGGCGTCTGGGGACAAGGAAACTCCACCAGCCGAAGGCGAGGGCCATGAGGCCCCCATT
GCCAAGAAGGAGATCAGCCTGCCCATCCTCTGCAATGCCTCCAATGGCAGTGCCGTGGGCGTTCCTGAC
AGGACTGTCTCCAGGCTGGCCCAGGGCTCTCCAGCTGTGGCCAGGATTGCCCCTCAGCGGGCCCAGATC
AGCTCCCCGAACCTACGGACAAGACCTCCACCACGCAGAGAATCCAGCCTGGGGTTCCAACTGCCAAAG
CCACCAGAGCCCCCTTCTGTTGAGGTGGAGTACTACACCATTGCCGAATTCCAGTCGTGCATTTCCGAT
GGCATCAGCTTTCGGGGTGGACAGAAGGCAGAGGTCATTGATAAGAACTCAGGTGGCTGGTGGTACGTG
CAGATCGGTGAGAAGGAGGGCTGGGCCCCCGCATCATACATCGATAAGCGCAAGAAGCCCAACCTGAGC
CGCCGCACAAGCACGCTGACCCGGCCCAAGGTGCCCCCGCCAGCACCCCCCAGCAAGCCCAAGGAGGCC
GAGGAGGGCCCTACGGGGGCCAGTGAGAGCCAGGACTCCCCGCGGAAGCTCAAGTATGAGGAGCCTGAG
TATGACATCCCTGCATTCGGCTTTGACTCAGAGCCTGAGCTGAGCGAGGAGCCCGTGGAGGACAGAGCC
TCAGGGGAGAGGCGGCCTGCCCAGCCCCACCGGCCCTCGCCGGCCTCTTCTCTGCAGCGGGCCCGCTTC
AAGGTGGGTGAGTCTTCAGAGGATGTGGCCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCCGG
CCATATGCAGAGGACACCCTGTCAGCCAGAGGCTCCTCCGGGGACAGCGACTCCCCAGGCAGCTCCTCG
CTGTCCCTGACCAGGAAAAACTCCCCCAAATCAGGCTCCCCCAAGTCATCATCACTCCTAAAGCTCAAG
GCAGAGAAGAATGCCCAGGCAGAAATGGGGAAGAACCACTCCTCAGCCTCCTTTTCCTCATCCATCACC
ATCAACACCACTTGCTGCTCCTCCTCTTCCTCCTCCTCCTCTTCCTTGTCCAAAACCAGTGGCGACCTG
AAGCCCCGCTCTGCTTCGGACGCAGGCATCCGCGGCACTCCCAAGGTCAGGGCAAAGAAGGATGCTGAT
GCGAACGCTGGGCTGACCTCCTGTCCCCGGGCCAAGCCATCGGTCCGGCCCAAGCCATTCCTAAACCGA
GCAGAGTCGCAGAGCCAAGAGAAGATGGACATCAGCACTTTACGGCGCCAGCTGAGACCCACAGGCCAG
CTCCGTGGAGGGCTCAAGGGCTCCAAGAGTGAGGATTCGGAGCTGCCCCCGCAGACGGCCTCCGAGGCT
CCCAGTGAGGGGTCTAGGAGAAGCTCATCCGACCTCATCACCCTCCCAGCCACCACTCCCCCATGTCCC
ACCAAGAAGGAATGGGAAGGGCCAGCCACCTCGTACATGACATGCAGCGCCTACCAGAAGGTCCAGGAC
TCGGAGATCAGCTTCCCCGCGGGCGTGGAGGTGCAGGTGCTGGAGAAGCAGGAGAGCGGGTGGTGGTAT
GTGAGGTTTGGGGAGCTGGAGGGCTGGGCCCCTTCCCACTATTTGGTGCTGGATGAGAACGAGCAACCT
GACCCCTCTGGCAAAGAGCTGGACACAGTGCCCGCCAAGGGCAGGCAGAACGAAGGCAAATCAGACAGC
CTGGAGAAGATCGAGAGGCGCGTCCAAGCACTGAACACCGTCAACCAGAGCAAGAAGGCCACGCCCCCC
ATCCCCTCCAAACCTCCCGGGGGCTTCGGCAAGACCTCAGGCACTCCAGCGGTGAAGATGAGGAACGGA
GTGCGGCAGGTGGCGGTCAGGCCCCAGTCGGTGTTTGTGTCCCCGCCACCCAAGGACAACAACCTGTCC
TGCGCCCTGCGGAGGAATGAGTCACTCACGGCCACTGATGGCCTCCGAGGCGTCCGACGGAACTCCTCC
TTTAGCACTGCTCGCTCCGCTGCCGCCGAGGCCAAGGGCCGCCTGGCCGAACGGGCTGCCAGCCAGGGT
TCAGACTCACCCCTACTGCCCGCCCAGCGCAACAGCATACCCGTGTCCCCTGTGCGCCCCAAGCCCATC
GAGAAGTCTCAGTTCATCCACAATAACCTCAAAGATGTGTACGTCTCTATCGCAGACTACGAGGGGGAT
GAGGAGACAGCAGGCTTCCAGGAGGGGGTGTCCATGGAGGTTCTGGAGAGGAACCCTAATGGCTGGTGG
TACTGCCAGATCCTGGATGGTGTGAAGCCCTTCAAAGGCTGGGTGCCTTCCAACTACCTTGAGAAAAAG
AACTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_014631
Insert Size 3318 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014631.2
RefSeq Size 11264 bp
RefSeq ORF 3318 bp
Locus ID 9644
UniProt ID Q5TCZ1
Cytogenetics 10q24.33
MW 121.8 kDa
Summary Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014631" in other vectors (6)
SKU Description Size Price
RC217487 SH3PXD2A (Myc-DDK-tagged)-Human SH3 and PX domains 2A (SH3PXD2A) 10 ug
$1,443.00
RC217487L1 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), Myc-DDK-tagged 10 ug
$1,743.00
RC217487L2 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), mGFP tagged 10 ug
$1,743.00
RC217487L3 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), Myc-DDK-tagged 10 ug
$1,743.00
RC217487L4 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), mGFP tagged 10 ug
$1,743.00
RG217487 SH3PXD2A (tGFP-tagged) - Human SH3 and PX domains 2A (SH3PXD2A) 10 ug
$1,643.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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