FISH (SH3PXD2A) (NM_014631) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC217487L3
Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), Myc-DDK-tagged
  $1,743.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FISH
Synonyms FISH; SH3MD1; TKS5
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC217487).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014631
ORF Size 3315 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014631.2, NP_055446.2
RefSeq Size 11264 bp
RefSeq ORF 3318 bp
Locus ID 9644
UniProt ID Q5TCZ1
Cytogenetics 10q24.33
MW 121.7 kDa
Summary Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014631" in other vectors (6)
SKU Description Size Price
RC217487 SH3PXD2A (Myc-DDK-tagged)-Human SH3 and PX domains 2A (SH3PXD2A) 10 ug
$1,443.00
RC217487L1 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), Myc-DDK-tagged 10 ug
$1,743.00
RC217487L2 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), mGFP tagged 10 ug
$1,743.00
RC217487L4 Lenti ORF clone of Human SH3 and PX domains 2A (SH3PXD2A), mGFP tagged 10 ug
$1,743.00
RG217487 SH3PXD2A (tGFP-tagged) - Human SH3 and PX domains 2A (SH3PXD2A) 10 ug
$1,643.00
SC304145 SH3PXD2A (untagged)-Human SH3 and PX domains 2A (SH3PXD2A) 10 ug
$1,540.00

Plasmid Map for RC217487L3

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Cloning scheme for RC217487L3

Circular map for RC217487L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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