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TEF (NM_003216) Human Untagged Clone

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SKU
SC303285
TEF (untagged)-Human thyrotrophic embryonic factor (TEF), transcript variant 1
  $330.00
In Stock*
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Other products for "TEF or NM_003216"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TEF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_003216
Insert Size 912 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003216.3
RefSeq Size 4404 bp
RefSeq ORF 912 bp
Locus ID 7008
UniProt ID Q10587
Cytogenetics 22q13.2
Protein Families Transcription Factors
MW 33.2 kDa
Summary This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2012
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_003216" in other vectors (4)
SKU Description Size Price
RC205147 TEF (Myc-DDK-tagged)-Human thyrotrophic embryonic factor (TEF), transcript variant 1 10 ug
$300.00
RC205147L3 Lenti ORF clone of Human thyrotrophic embryonic factor (TEF), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC205147L4 Lenti ORF clone of Human thyrotrophic embryonic factor (TEF), transcript variant 1, mGFP tagged 10 ug
$600.00
RG205147 TEF (tGFP-tagged) - Human thyrotrophic embryonic factor (TEF), transcript variant 1 10 ug
$489.00 $500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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