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CTRB2 (NM_001025200) Human Untagged Clone

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SKU
SC302364
CTRB2 (untagged)-Human chymotrypsinogen B2 (CTRB2)
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CTRB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC302364 representing NM_001025200.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTTTCCTCTGGCTCCTCTCCTGCTGGGCCCTCCTGGGTACCACCTTCGGCTGCGGGGTCCCCGCC
ATCCACCCTGTGCTCAGCGGCCTGTCCAGGATCGTGAATGGGGAGGACGCCGTCCCCGGCTCCTGGCCC
TGGCAGGTGTCCCTGCAGGACAAAACCGGCTTCCACTTCTGCGGGGGCTCCCTCATCAGCGAGGACTGG
GTGGTCACCGCTGCCCACTGCGGGGTCAGGACCTCCGACGTGGTCGTGGCTGGGGAGTTTGACCAGGGC
TCTGACGAGGAGAACATCCAGGTCCTGAAGATCGCCAAGGTCTTCAAGAACCCCAAGTTCAGCATTCTG
ACCGTGAACAATGACATCACCCTGCTGAAGCTGGCCACACCTGCCCGCTTCTCCCAGACAGTGTCCGCC
GTGTGCCTGCCCAGCGCCGACGACGACTTCCCCGCGGGGACACTGTGTGCCACCACAGGCTGGGGCAAG
ACCAAGTACAACGCCAACAAGACCCCTGACAAGCTGCAGCAGGCAGCCCTGCCCCTCCTGTCCAATGCC
GAATGCAAGAAGTCCTGGGGCAGGAGGATCACCGACGTGATGATCTGTGCCGGGGCCAGTGGCGTCTCC
TCCTGCATGGGTGACTCTGGAGGCCCCCTGGTCTGCCAGAAGGACGGAGCCTGGACCCTGGTGGGCATT
GTGTCCTGGGGCAGCCGCACCTGCTCTACCACCACGCCCGCTGTGTACGCCCGTGTCGCCAAGCTCATA
CCCTGGGTGCAGAAGATCCTGGCCGCCAACTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001025200
Insert Size 792 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001025200.3
RefSeq Size 936 bp
RefSeq ORF 792 bp
Locus ID 440387
UniProt ID Q6GPI1
Cytogenetics 16q23.1
Protein Families Druggable Genome, Transmembrane
MW 27.9 kDa
Summary This gene encodes a member of the serine protease family of enzymes and forms a principal precursor of the pancreatic proteolytic enzymes. The encoded preproprotein is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate a functional enzyme. This CTRB2 gene is located head-to-head with the related CTRB1 gene. Some human populations have an alternate haplotype which inverts a 16.6 Kb region containing portions of intron 1, exon 1, and the upstream sequence of the CTRB1 and CTRB2 genes. In this inversion haplotype exon 1 and flanking sequence is swapped in CTRB1 and CTRB2. This inversion is associated with differential gene expression and increased risk for chronic pancreatitis. The GRCh38 assembly represents the minor allele for SNP rs8048956 of the CTRB1 gene. SNP rs8048956 is diagnostic for this inversion. provided by RefSeq, Jan 2021
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"NM_001025200" in other vectors (4)
SKU Description Size Price
RC209957 CTRB2 (Myc-DDK-tagged)-Human chymotrypsinogen B2 (CTRB2) 10 ug
$300.00
RC209957L3 Lenti ORF clone of Human chymotrypsinogen B2 (CTRB2), Myc-DDK-tagged 10 ug
$600.00
RC209957L4 Lenti ORF clone of Human chymotrypsinogen B2 (CTRB2), mGFP tagged 10 ug
$600.00
RG209957 CTRB2 (tGFP-tagged) - Human chymotrypsinogen B2 (CTRB2) 10 ug
$500.00

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