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COX7A1 (NM_001864) Human Untagged Clone

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SKU
SC118989
COX7A1 (untagged)-Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol COX7A1
Synonyms COX7A; COX7AH; COX7AM
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118989 sequence for NM_001864 edited (data generated by NextGen Sequencing)
ATGCAGGCCCTTCGGGTGTCCCAGGCGCTGATCCGCTCCTTCAGCTCCACCGCCCGGAAC
CGCTTTCAGAACCGAGTGCGCGAGAAACAGAAGCTCTTCCAGGAGGACAATGACATCCCG
TTGTACCTGAAGGGCGGCATCGTTGACAACATCCTGTACCGAGTGACAATGACGCTGTGT
CTGGGCGGCACTGTCTACAGCTTGTACTCCCTTGGCTGGGCCTCCTTCCCCAGGAATTAA

Clone variation with respect to NM_001864.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001864 unedited
NGCGTTCGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGACAAGG
CAGAATGCAGGCCCTTCGGGTGTCCCAGGCGCTGATCCGCTCCTTCAGCTCCACCGCCCG
GAACCGCTTTCAGAACCGAGTGCGCGAGAAACAGAAGCTCTTCCAGGAGGACAATGACAT
CCCGTTGTACCTGAAGGGCGGCATCGTTGACAACATCCTGTACCGAGTGACAATGACGCT
GTGTCTGGGCGGCACTGTCTACAGCTTGTACTCCCTTGGCTGGGCCTCCTTCCCCAGGAA
TTAAGACCAAGAAGCCTGGGGGGCCTGAGAGACTTGAACAAGTGTCAATAAACGCTGGCC
TCTGAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAG
CTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGG
CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCA
TTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGTGGAGGGGGGGTTGGGNNNNNNC
NNNNNNNNNNNCNNNNCNNCCNNNCNNNNNNNNNNNNNCNNCNNNCCCCCCNCCCCCNCC
CCCCCCCCCCCCCCCCCCCCCNNCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCTTCCCCCCCCCCCC
CCCCCCCCCCCCCACCCCCNCCCCCCCCCCNNCNCNNCCNCCCCCNNNCCCCCCCCNCCC
CCCCCCCCCCCCCCCCCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001864 unedited
GCGCGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTCAGAGGCCAGC
GTTTATTGACACTTGTTCAAGTCTCTCAGGCCCCCCAGGCTTCTTGGTCTTAATTCCTGG
GGAAGGAGGCCCAGCCAAGGGAGTACAAGCTGTAAACAGTGCCGCCCAAACACAGCGTCA
TTGTCACTCGGTACAGGATGTTGTCAACGATGCCGCCCTTCAGGTACAACGGGATGTCAT
TGTCCTCCTGGAAGAGCTTCTGTTTCTCGCGCACTCGGTTCTGAAAGCGGTTCCGGGCGG
TGGAGCTGAAGGAGCGGATCAGCGCCTGGGACACCCGAAGGGCCTGCATTCTGCCTTGTC
CCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCA
CTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCA
ACGGGGCGGNGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAAC
TCCCATTGACGTCAATGGGGTGGAGACTTGGAATTCCCCGTGAGTCAAACCGCTATCCAC
GCCCATTGGTGTACTGCCCAAACCGCATCACCATGGTAATAGCGATGACTAATACGTANA
TGTACTGCCAAGTAGGAAAGTCCCGTAAGGTCATGTACTGGGCATAATGCCAAGCGGGCC
ATTTACCGTCATTGACGTCAATAGGGGGGCGGACTTGGCATATGATACACTTGATGGACT
GGCCAGTGGGCAGTTTACCGGAATACTCCCCCCATTGACGTCAATGGAAGGCCCTATTGC
GTTCCTTGGAAACATACCTATTATTGANTCAATGGCCGGGGTTTTGGGCGGCAACCCGCG
GGCTTTACCCGAAGTTTGTACCCGGACTCCATATTGGCATTGACTATGACCCGTAATGAT
TCTANTAAACTGGCATATCATGCAACTGCGGGCATTTGACTGGGCATTANTGCCTTTTGA
TTAAAACACGTTGCATGGCATACCATTTGTTTTGCTTAACC
Restriction Sites NotI-NotI |
ACCN NM_001864
Insert Size 380 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001864.2, NP_001855.1
RefSeq Size 783 bp
RefSeq ORF 240 bp
Locus ID 1346
UniProt ID P24310
Cytogenetics 19q13.12
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Oxidative phosphorylation, Parkinson's disease
Summary Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (muscle isoform) of subunit VIIa and the polypeptide 1 is present only in muscle tissues. Other polypeptides of subunit VIIa are present in both muscle and nonmuscle tissues, and are encoded by different genes. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001864" in other vectors (4)
SKU Description Size Price
RC201154 COX7A1 (Myc-DDK-tagged)-Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein 10 ug
$150.00
RC201154L3 Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC201154L4 Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG201154 COX7A1 (tGFP-tagged) - Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein 10 ug
$489.00

file-alt Citations

Downregulation of cytochrome c oxidase subunit 7A1 expression is important in enhancing cell proliferation in adenocarcinoma cells
Mishra, Timilsina, Ghimire et al
Biochem. Biophys. Res. Commun. (2016)
AI Summary: OriGene's human COX7A1 ORF plasmid (catalog: SC118989) was used to overexpress COX7A1 in lung cancer cells, enabling the study to demonstrate that increased COX7A1 expression inhibits cell proliferation and promotes apoptosis in adenocarcinoma cells.
Snippet: At approximately 70% confluency, cells were transfected with 3 g of full-lengthhuman COX7A1 ORF plasmid DNA (Origene, USA;catalog: SC118989) using X-treme GeneHP DNA transfection reagent (Roche, Germany) as per manufacturer's protocol. 2.3. qPCR ... Cite SaveSaving
Showing 1-1 of 1 papers.
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