IFITM1 (NM_003641) Human Untagged Clone

SKU SC117830

IFITM1 (untagged)-Human interferon induced transmembrane protein 1 (9-27) (IFITM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: IFITM1
• Synonyms: 9-27; CD225; DSPA2a; IFI17; LEU13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117830 sequence for NM_003641 edited (data generated by NextGen Sequencing); ATGCACAAGGAGGAACATGAGGTGGCTGTGCTGGGGGCACCCCCCAGCACCATCCTTCCA; AGGTCCACCGTGATCAACATCCACAGCGAGACCTCCGTGCCCGACCATGTCGTCTGGTCC; CTGTTCAACACCCTCTTCTTGAACTGGTGCTGTCTGGGCTTCATAGCATTCGCCTACTCC; GTGAAGTCTAGGGACAGGAAGATGGTTGGCGACGTGACCGGGGCCCAGGCCTATGCCTCC; ACCGCCAAGTGCCTGAACATCTGGGCCCTGATTCTGGGCATCCTCATGACCATTGGATTC; ATCCTGTTACTGGTATTCGGCTCTGTGACAGTCTACCATATTATGTTACAGATAATACAG; GAAAAACGGGGTTACTAG; ; Clone variation with respect to NM_003641.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003641 unedited; ATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCTCACTGAGCACCG; TCCCAGCATCCGGACACCACAGCGGCCCTTCGCTCCACGCAGAAAACCACACTTCTCAAA; CCTTCACTCAACACTTCCTTCCCCAAAGCCAGAAGATGCACAAGGAGGAACATGAGGTGG; CTGTGCTGGGGGCACCCCCCAGCACCATCCTTCCAAGGTCCACCGTGATCAACATCCACA; GCGAGACCTCCGTGCCCGACCATGTCGTCTGGTCCCTGTTCAACACCCTCTTCTTGAACT; GGTGCTGTCTGGGCTTCATAGCATTCGCCTACTCCGTGAAGTCTAGGGACAGGAAGATGG; TTGGCGACGTGACCGGGGCCCAGGCCTATGCCTCCACCGCCAAGTGCCTGAACATCTGGG; CCCTGATTCTGGGCATCCTCATGACCATTGGATTCATCCTGTTACTGGTATTCGGCTCTG; TGACAGTCTACCATATTATGTTACAGATAATACAGGAAAAACGGGGTTACTAGTAGCCGC; CCATAGCCTGCAACCTTTGCACTCCACTGTGCAATGCTGGCCCTGCACGCTGGGGCTGTT; GCCCCTGCCCCCTTGGTCCTGCCCCTAGATACAGCAGTTTATACCCACACACCTGTCTAC; AGTGTCATTCAATAAAGTGCACGTGCTTGTGAANAANAAAAANNNNNNNNANNANAAAAA; CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATC; CCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCCTGNAAAGTGCCACTCCAGTGCCCACC; AGCCTTGTCCTAATAAAAATAAAGTGCATCATTTTGTCTGACTAGGGGTNCCTCTATATA; TATGGTGGAGGGGGGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003641 unedited; GGCTTACTTGNACGCGGCCGCATTCTANATCGAGNTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTAACAACCACGTGCACTTTATTGAATGACACTGTAAACAGGTGTGTGGGTATAAACT; GCTGTATCTAGGGGCAGGACCAAGGGGGCAGGGGCAACAGCCCCAGCGTGCAGGGCCAGC; ATTGCACAGGGGAGTGCAAAGGTTGCAGGCTATGGGCGGTTACTAGTAACCCCGTTTTTC; CTGTATTATCTGCAACATAATATGGTAAACTGTCACAGAGCCGAATACCAGTAACAGGAT; GAATCCAATGGTCATGAGGATGCCCAAAATCAGGGCCCAAATGTTCAGGCACTTGGCGGG; GGAGGCATAGGCCTGGGCCCCGGTCACGTCGCCAACCATCTTCCTGTCCCTAAACTTAAC; GGAGTAGGCGAATGCTATGAAGCCCAAACAGCACCAGTTCAAAAAAAGGGTGTTGAACAG; GGACCAAACAACATGGCCGGGCACGGAGGTCTTGTTGTGGATGTTGATCACGGCGGACCT; TGAAAGGATGGTGCTGGGGGGGTCCCCTCCGCACACTCACCTTCCCGTCTTTCCTTGTGC; CCCTCCTTGCTCCGCCGAACGTACTTTTCCTCCACCCCTTCCCCCTCTCCCTCTCCCCCC; CCCCTCCCCTCTCTTCTTTCTTTCTTCCTACCATTCTTTACTCCCATCCTTCCCCTCCCT; TCTTTCCTTCCCTTTCCCCCTCATCCCTTCATTCCCTTCTTCTCCTTTTCCCCCCCACCA; CCCCTCCCTCTTATCCCCCTCCACTATCATATCCCATCCATCCTCTACCTTTCCCCCCTA; TTCCCTTTCTCCTCCTCTCCTCCTCCCCAATACTTCTCTCATTCAGCACGTCTCCCCCCT; TCCCCTCAATTCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003641
• Insert Size: 1180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003641.2, NP_003632.2
• RefSeq Size: 853 bp
• RefSeq ORF: 378 bp
• Locus ID: 8519
• UniProt ID: P13164
• Cytogenetics: 11p15.5
• Domains: CD225
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway
• Summary: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation.[UniProtKB/Swiss-Prot Function]