IFITM1 (NM_003641) Human Tagged ORF Clone
SKU
RC201617
IFITM1 (Myc-DDK-tagged)-Human interferon induced transmembrane protein 1 (9-27) (IFITM1)
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IFITM1 |
| Synonyms | 9-27; CD225; DSPA2a; IFI17; LEU13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003641 |
| ORF Size | 375 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003641.4 |
| RefSeq Size | 733 bp |
| RefSeq ORF | 378 bp |
| Locus ID | 8519 |
| UniProt ID | P13164 |
| Cytogenetics | 11p15.5 |
| Domains | CD225 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | B cell receptor signaling pathway |
| MW | 13.9 kDa |
| Summary | IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003641" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201617L1 | Lenti ORF clone of Human interferon induced transmembrane protein 1 (9-27) (IFITM1), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC201617L2 | Lenti ORF clone of Human interferon induced transmembrane protein 1 (9-27) (IFITM1), mGFP tagged | 10 ug |
$450.00
|
|
| RC201617L3 | Lenti ORF clone of Human interferon induced transmembrane protein 1 (9-27) (IFITM1), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC201617L4 | Lenti ORF clone of Human interferon induced transmembrane protein 1 (9-27) (IFITM1), mGFP tagged | 10 ug |
$450.00
|
|
| RG201617 | IFITM1 (tGFP-tagged) - Human interferon induced transmembrane protein 1 (9-27) (IFITM1) | 10 ug |
$489.00
|
|
| SC117830 | IFITM1 (untagged)-Human interferon induced transmembrane protein 1 (9-27) (IFITM1) | 10 ug |
$150.00
|
|
Citations
IFITM1 enhances nonenveloped viral RNA replication by facilitating cholesterol transport to the Golgi
Ishikawa-Sasaki, Murata, Sasaki
PLoS Pathog (2023) 19 (5), e1011383 DOI: 10.1371/journal.ppat.1011383
Ishikawa-Sasaki, Murata, Sasaki
PLoS Pathog (2023) 19 (5), e1011383 DOI: 10.1371/journal.ppat.1011383
Cited by 6
AI Summary: OriGene cDNA clones for IFITM1 (RC201617), IFITM2 (RC202067), and IFITM3 (RC201635) were used to generate expression constructs that enabled the study of IFITM proteins’ roles in cholesterol transport and Aichi virus RNA replication in this paper.
Snippet: A-SAC1, and pCMV-polyprotein was described previously [6,37]. The coding regions of IFITM1, IFITM2, and IFITM3 were amplified by PCR using primer pairs containing MluI–EcoRV sites from cDNA clones (OriGene;IFITM1: RC201617, IFITM2: RC202067, IFITM3: RC201635), respectively, and then inserted into the same sites of pACT and pBIND, resulting in pACT-IFITM1, pACT-IFITM2, pACT-IFITM3, pBIND-IFITM1,
Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity
Li, Liu, Liu et al
Nat. Immunol. (2013) 14 (8), 793-803 DOI: 10.1038/ni.2647
Li, Liu, Liu et al
Nat. Immunol. (2013) 14 (8), 793-803 DOI: 10.1038/ni.2647
Cited by 513
AI Summary: OriGene products, specifically the IFNA1 cDNA clone (Cat# SC117416) and GAPDH antibody (Cat# TA802519), were used in this study to investigate the expression and detection of interferon-α and control proteins, thereby supporting the analysis of exosome-mediated antiviral activity.
ISG56 and IFITM1 proteins inhibit hepatitis C virus replication
Raychoudhuri, Shrivastava, Steele et al
J. Virol. (2011) 85 (24), 12881-9 DOI: 10.1128/JVI.05633-11
Raychoudhuri, Shrivastava, Steele et al
J. Virol. (2011) 85 (24), 12881-9 DOI: 10.1128/JVI.05633-11
Cited by 19
AI Summary: OriGene products pCDNA3-Myc-tagged human ISG56 and pCMV6-Myc-DDK-tagged human IFITM1 ORF clones were used for exogenous expression studies to demonstrate that these proteins inhibit hepatitis C virus replication.
Snippet: Thus, there is an urgent need to identify novel antivirals or cellular molecules that can be used for therapy ... μg of a pCDNA3-Myc-tagged open reading frame (ORF) clone of human ISG56 and a pCMV6-Myc-DDK-tagged ORF clone of human IFITM1 (Origene) using Lipofectamine
Showing 1-3 of 3 papers.
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