ASCL1 (NM_004316) Human Untagged Clone

SKU SC117427

ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ASCL1
• Synonyms: ASH1; bHLHa46; HASH1; MASH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004316, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCCGCAGCCCCAGCAGCCCT; TCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGCGGCGGCCGCAGCCGCCGCAGCGGCAGC; GCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCC; GACGGCCAGCCCTCAGGGGGCGGTCACAAGTCAGCGCCCAAGCAAGTCAAGCGACAGCGCTCGTCTTCGC; CCGAACTGATGCGCTGCAAACGCCGGCTCAACTTCAGCGGCTTTGGCTACAGCCTGCCGCAGCAGCAGCC; GGCCGCCGTGGCGCGCCGCAACGAGCGCGAGCGCAACCGCGTCAAGTTGGTCAACCTGGGCTTTGCCACC; CTTCGGGAGCACGTCCCCAACGGCGCGGCCAACAAGAAGATGAGTAAGGTGGAGACACTGCGCTCGGCGG; TCGAGTACATCCGCGCGCTGCAGCAGCTGCTGGACGAGCATGACGCGGTGAGCGCCGCCTTCCAGGCAGG; CGTCCTGTCGCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCCATGGCCGGCTCGCCGGTCTCA; TCCTACTCGTCGGACGAGGGCTCTTACGACCCGCTCAGCCCCGAGGAGCAGGAGCTTCTCGACTTCACCA; ACTGGTTCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004316 unedited; TGTTANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGGCCAGG; GAACGTGGAAGGCGCACCGACAGGNATCCGGCCAGGGAGGGCGAGTGAAAGAAGGAAATC; AGAAAGGAAGGGAGTTAACAAAATAATAAAAACAGCCTGAGCCACGGCTGGAGAGACCGA; GACCCGGCGCAAGAGAGCGCAGCCTTAGTAGGAGAGGAACGCGAGACGCGGCAGAGCGCG; TTCAGCACTGACTTTTGCTGCTGCTTCTGCTTTTTTTTTTCTTAGAAACAAGAAGGCGCC; AGCGGCAGCCTCACACGCGAGCGCCACGCGAGGCTCCCGAAGCCAACCCGCGAAGGGAGG; AGGGGAGGGAGGAGGAGGCGGCGTGCAGGGAGGAGAAAAAGCATTTTCACTTTTTTTGCT; CCCACTCTAAGAAGTCTCCCGGGGATTTTGTATATATTTTTTAACTTCCGTCAGGGCTCC; CGCTTCATATTTCCTTTTCTTTCCCTCTCTGTTCCTGCACCCAAGTTCTCTCTGTGTCCC; CCTCGCGGGCCCCGCACCTCGCGTCCCGGATCGCTCTGATTCCGCGACTCCTTGGCCGCC; GCTGCGCATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCC; GCAGCCCCAGCAGCCCTTCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGC; CGGCGGCCGCAGCCGCCGCAGCGGCAGCGCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGC; AGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCCGACGCCAGCCTCAGGGGGCCGG; TCACAAGTCAGCGCCCAAGCAGTCAAGCGAAGCGCTCGTCTTCGNCCGACTGATGCGCTG; CAACGCCGCTCACTCAGCGGCTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004316 unedited; ACTTAAAGAGGGAAAAGACTTATTACTGAACTAGTTTGTATAAAACAGGGTTATACATAT; TTTTGTAAGTTTGTAATAAAACAGTAAGAAAAAAAAGCAGTAATAGAAATCTCCAAAAGG; CAACCTATCAAAACCAACTGGCTGCCACTTTGAGTTTGGACAGTAGCTGCATAAACTTTG; TTCTTCTTGAACAGTATTTAATAACATCATTAATACATTAACAACATTTCTATAAAGTAA; GACACATTGGTGCTGAAGTACAACTGGTGGCCTCTTGATCTCACCTATGAGGAGAGTTCT; TTACAAAACCACATAGGGAAAATTGCAGTTGTAAGGTGAACTACACATCTAAAATATGCA; GAGGTAATAGCATTACATGTTAAAGTATCAAGATATACACATTTTAAACCATTTGTACAA; AACTTCTATAAATTTTTCTCTCTCTTTCTCTCTTATGTACAAAAATATCTTAATATATCC; CCGAACTGGTTAGGATAGATACAAATAGATTTTTTATAATAAAAAATTCACAAAAGATTG; GAAGCATTCTATAATGAAAATGGTAGAAAAGACAGTGTGAGGGAAGCCATGGGGTTTGGG; AATCGGGCCCTGGAGGAGAAGCAGAGTTTCAAAGGGCTGAGAATAGCATAGTTTCACTGT; AAACCAATGTCTACAGCTTATTGGGGTGGGGGCTACTGAGACGAAAGACACCAACTTCGT; TTCTAGAAGGCTAAGAACTGCCCTCTAAGAAGGGCGGCGAGGTGAAGGGACCCGAGCATG; ACCTTCAGCTGTGCCGTGTATAGTGATGGGAGTACTATAACGCGTGTGCTGCTCCCTTCT; GCCAGAACCCACTGCTTTTGCCACCAGCTGCATTTTGCGCATTGACTCAGTTCCAGTTGC; TCTCTTATCTCCCGGATGATTGAGAGCAAAAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004316
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004316.2, NP_004307.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 711 bp
• Locus ID: 429
• UniProt ID: P50553
• Cytogenetics: 12q23.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]