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ASCL1 (NM_004316) Human Tagged ORF Clone

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SKU
RC201123
ASCL1 (Myc-DDK-tagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ASCL1 or NM_004316"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ASCL1
Synonyms ASH1; bHLHa46; HASH1; MASH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201123 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCCGCAGCCCCAGCAGCCCT
TCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGCGGCGGCCGCAGCCGCCGCAGCGGCAGC
GCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCC
GACGGCCAGCCCTCAGGGGGCGGTCACAAGTCAGCGCCCAAGCAAGTCAAGCGACAGCGCTCGTCTTCGC
CCGAACTGATGCGCTGCAAACGCCGGCTCAACTTCAGCGGCTTTGGCTACAGCCTGCCGCAGCAGCAGCC
GGCCGCCGTGGCGCGCCGCAACGAGCGCGAGCGCAACCGCGTCAAGTTGGTCAACCTGGGCTTTGCCACC
CTTCGGGAGCACGTCCCCAACGGCGCGGCCAACAAGAAGATGAGTAAGGTGGAGACACTGCGCTCGGCGG
TCGAGTACATCCGCGCGCTGCAGCAGCTGCTGGACGAGCATGACGCGGTGAGCGCCGCCTTCCAGGCAGG
CGTCCTGTCGCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCCATGGCCGGCTCGCCGGTCTCA
TCCTACTCGTCGGACGAGGGCTCTTACGACCCGCTCAGCCCCGAGGAGCAGGAGCTTCTCGACTTCACCA
ACTGGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201123 protein sequence
Red=Cloning site Green=Tags(s)

MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAA
DGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFAT
LREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVS
SYSSDEGSYDPLSPEEQELLDFTNWF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004316
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004316.4
RefSeq Size 2490 bp
RefSeq ORF 711 bp
Locus ID 429
UniProt ID P50553
Cytogenetics 12q23.2
Domains HLH
Protein Families Druggable Genome, Transcription Factors
MW 25.5 kDa
Summary This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. provided by RefSeq, Jul 2008
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"NM_004316" in other vectors (7)
SKU Description Size Price
RC201123L1 Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), Myc-DDK-tagged 10 ug
$600.00
RC201123L2 Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), mGFP tagged 10 ug
$600.00
RC201123L3 Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), Myc-DDK-tagged 10 ug
$600.00
RC201123L4 Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), mGFP tagged 10 ug
$600.00
RG201123 ASCL1 (tGFP-tagged) - Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) 10 ug
$489.00 $500.00
SC117427 ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) 10 ug
$300.00
SC324383 ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) 10 ug
$300.00

Plasmid Map for RC201123

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201123
Circular map for RC201123

file-alt Citations

Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium
Joppé, Cochard, Levros et al
Life Sci Alliance (2020) 3 (7) DOI: 10.26508/lsa.202000743
Cited by 5
AI Summary: OriGene products contributed to this study by providing the Mash1 (Ascl1) cDNA clone (OriGene Cat# MR207345) used for transient overexpression experiments to assess the neurogenic potential of GFAP+ precursors in vivo.
FigureFigureFigure
Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells
Kudoh, Tenjin, Kameyama et al
Histochem. Cell Biol. (2020)
Cited by 39
AI Summary: OriGene’s human ASCL1 cDNA ORF clone was used to construct the ASCL1 expression plasmid (pCAG-IRES-Puro-ASCL1), enabling the study of ASCL1’s functional role in pulmonary neuroendocrine carcinoma cells.
Snippet: Bank (Osaka, Japan). Plasmid construction. To construct pCAG-IRES-Puro- ASCL1, we replicated the ASCL1 gene of a human ASCL1 cDNA ORF clone (OriGene, Rockville, Maryland) and replaced it with ASCL1. We generated
Combinatorial programming of human neuronal progenitors using magnetically-guided stoichiometric mRNA delivery
Azimi, Sheridan, Ghannad-Rezaie et al
Elife (2018) 7 DOI: 10.7554/eLife.31922
AI Summary: OriGene’s pCMV6 cDNA clones for OTX2, LMX1A, FOXA2, ASCL1, NGN2, NURR1, PITX3, EGFP, and mCherry were used as templates to generate mRNAs for combinatorial transcription factor delivery in neural progenitor cell differentiation experiments.
Snippet: has been previously described (Angel and Yanik, 2010). Briefly, dsDNA templates were linearized from cDNA clones in pCMV6 vectors for OTX2, LMX1A, FOXA2, ASCL1, NGN2, NURR1, PITX3, EGFP, and mCherry (OriGene, Rockville, MD). To maximize the stability of mRNA transcripts and increase protein translation, we added 5’ and 3’ untranslated regions from human Beta-globin gene (Hbb) to the templates
FigureFigureFigure
Showing 1-3 of 3 papers.
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