CDK2AP1 (NM_004642) Human Untagged Clone

SKU SC117244

CDK2AP1 (untagged)-Human cyclin-dependent kinase 2 associated protein 1 (CDK2AP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CDK2AP1
• Synonyms: doc-1; DOC1; DORC1; p12DOC-1; ST19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117244 sequence for NM_004642 edited (data generated by NextGen Sequencing); ATGTCTTACAAACCGAACTTGGCCGCGCACATGCCCGCCGCCGCCCTCAACGCCGCTGGG; AGTGTCCACTCGCCTTCCACCAGCATGGCAACGTCTTCACAGTACCGCCAGCTGCTCAGT; GACTACGGGCCACCGTCCCTAGGCTACACCCAGGGAACTGGGAACAGCCAGGTGCCCCAA; AGCAAATACGCGGAGCTGCTGGCCATCATTGAAGAGCTGGGGAAGGAGATCAGACCCACG; TACGCAGGGAGCAAGAGTGCCATGGAGAGGCTGAAGCGCGGCATCATTCACGCTAGAGGA; CTGGTTCGGGAGTGCTTGGCAGAAACGGAACGGAATGCCAGATCCTAG; ; Clone variation with respect to NM_004642.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004642 unedited; GGTTCAAAATTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCTCGTGCC; GAATTCGGCACGAGGGCCGCCCGGGGGGATGTCTTACAAACCGAACTTGGCCGCGCACAT; GCCCGCCGCCGCCCTCAACGCCGCTGGGAGTGTCCACTCGCCTTCCACCAGCATGGCAAC; GTCTTCACAGTACCGCCAGCTGCTCAGTGACTACGGGCCACCGTCCCTAGGCTACACCCA; GGGAACTGGGAACAGCCAGGTGCCCCAAAGCAAATACGCGGAGCTGCTGGCCATCATTGA; AGAGCTGGGGAAGGAGATCAGACCCACGTACGCAGGGAGCAAGAGTGCCATGGAGAGGCT; GAAGCGCGGCATCATTCACGCTAGAGGACTGGTTCGGGAGTGCTTGGCAGAAACGGAACG; GAATGCCAGATCCTAGCTGCCTTGTTGGTTTTGAAGGATTTCCATCTTTTTACAAGATGA; GAAGTTACAGTTCATCTCCCCTGTTCAGATGAAACCCTTGTTTTCAAAATGGTTACAGTT; TCGTTTTTCCTCCCATGGTTCACTTGGCTCTGAACCTACAGTCTCAAAGATTGAGAAAAG; ATTTTGCAGTTAATTAGGATTTGCATTTTAAGTAGTTAGGAACTGCCCNAGTTTTTTTTG; TTTTTTAAGCATTGATTTAAAAGATGCACGGGAAAGTATCTTACAGCANACTGTAGTTTG; CCTCCAAGACACCCATGTCTCCCCTTAAAATCTCTCTTTGGATACATTTTGTACCCATGG; GGTGTCTTGNTCCNTTTCATAGCTAATACACTGTAGGGTNATTTGTTTTGACGCATATTG; ACAGCACGCTTTACTTAGTAGCCGGTTCCCATTTGCCATACAATGTAAGGNTCTGCTAAT; GTACTNCTTTTTTGGCTAAGCATTGCATGACTATTAATGGCTTCAAGTCAATTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004642 unedited; NNNNNAAAANNNNNTTTTCTTTAGGANCGCGGNNCCGCATTCTANNGATCGGTTTTTTTT; TTTTTTTTTTTTTTTAATAAAAATAAGCTTTATTACATCAAGTAATAAATACATACAAAG; ATGCAAACAGTTTTAGTCATTTTCTTCCAGATGTTTTTATCAACTTACAATAAACGCAGA; ACTGAGATCTACTTACAGTCTTAGTATGAAAGTGTTCGGGGGTCCTTGTTAGGTTTGGTG; GGTTGCTCTTTCTTCTGTATTTATAACTTGTGCATTTTTAAAAATTGACTTTGAAGCACT; AATAGTCATGCAAATGCTTAAGCAAAAAAGAAGTACATTAAGCAGAACCTACATTGTATG; GCAAATGGGAACCCGCTACTAAGTAAAGCGTGCTGTCAATATGCGTTCAAAACAAAATCC; CTACAGTGGTATTTAGCTTATGAAAGGGACAAAGANCACCNTGGGTAACAAATGTTTTCC; AAAAGAGAAGATAAAGGGAGACCATGTTGTCTTGGAGCAAACTACCGTTTGCTGTAAAAT; AACTTTCCGGGCATCTTTTAAATCAATGCTTAAAAAACAAAAAAACCTGGGCAAGTCCCT; ACTTACTAAATGGCAATCCTAATATACCGCAAAACTTTTTTAATCTTTTAACTTGAGGGT; CAAAGCCAGTAACCTTGGGAGGAAAAACAAACTGTGACCCTTTGAAAACAAGGTTTATTT; TAACGGGGGAAAGAACTGGACCTCTTATTTGGAAAAGAGGGAACCTTTAAAACCCCAGGG; CGTAGGATTGGGGTTCCGTCCCTTTTTGGGAAACCTCCCCACAAGCCTTTTAGGGAAAGG; CCCCGGTTAACCTCTCAGGGGCTTGGCCCCGTGGACGGGGGGTAATCCTTTCCCCTTTTA; AAAGGGGCCCCCTCCCTTTTTTTTTTGGGCCCGGGGGTCCCCACCTGGGGGAACCGGGAG; GGGGGCCCTAATTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004642
• Insert Size: 1180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004642.2, NP_004633.1
• RefSeq Size: 1627 bp
• RefSeq ORF: 348 bp
• Locus ID: 8099
• UniProt ID: O14519
• Cytogenetics: 12q24.31
• Summary: The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1).