HPGDS (NM_014485) Human Untagged Clone

SKU SC114983

HPGDS (untagged)-Human hematopoietic prostaglandin D synthase (HPGDS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HPGDS
• Synonyms: GSTS; GSTS1; GSTS1-1; PGD2; PGDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114983 sequence for NM_014485 edited (data generated by NextGen Sequencing); ATGCCAAACTACAAACTCACTTATTTTAATATGAGGGGGAGAGCAGAAATTATTCGTTAC; ATATTTGCTTATTTGGACATACAGTATGAAGACCACAGAATAGAACAAGCTGACTGGCCT; GAAATCAAATCAACTCTCCCATTTGGAAAAATCCCCATTTTGGAAGTTGATGGACTTACT; CTTCACCAGAGCCTAGCAATAGCAAGATATTTGACCAAAAACACAGATTTGGCTGGAAAC; ACAGAAATGGAACAATGTCATGTTGATGCTATTGTGGACACTCTGGATGATTTCATGTCA; TGTTTTCCTTGGGCAGAGAAAAAGCAAGATGTGAAAGAGCAGATGTTCAATGAGCTGCTC; ACGTATAATGCGCCTCATCTTATGCAAGACTTGGACACATATTTAGGGGGGAGAGAATGG; CTTATTGGTAACTCTGTAACTTGGGCAGACTTCTACTGGGAGATTTGCAGTACCACACTT; TTGGTCTTTAAGCCTGACCTGTTAGACAACCATCCAAGGCTGGTGACTTTACGGAAGAAA; GTCCAAGCCATTCCTGCCGTCGCTAACTGGATAAAACGAAGGCCCCAAACCAAACTCTAG; ; Clone variation with respect to NM_014485.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014485 unedited; TGCGGATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGACTTACTCGTC; ATATAAACCCAAGGCACAGTCACATACCCAGGGATACAAGACACTGCAGACTCCCGAGAG; ACATAACACAGAATTGCACCATGCCAAACTACAAACTCACTTATTTTAATATGAGGGGGA; GAGCAGAAATTATTCGTTACATATTTGCTTATTTGGACATACAGTATGAAGACCACAGAA; TAGAACAAGCTGACTGGCCTGAAATCAAATCAACTCTCCCATTTGGAAAAATCCCCATTT; TGGAAGTTGATGGACTTACTCTTCACCAGAGCCTAGCAATAGCAAGATATTTGACCAAAA; ACACAGATTTGGCTGGAAACACAGAAATGGAACAATGTCATGTTGATGCTATTGTGGACA; CTCTGGATGATTTCATGTCATGTTTTCCTTGGGCAGAGAAAAAGCAAGATGTGAAAGAGC; AGATGTTCAATGAGCTGCTCACGTATAATGCGCCTCATCTTATGCAAGACTTGGACACAT; ATTTAGGGGGGAGAGAATGGCTTATTGGTAACTCTGTAACTTGGGCAGACTTCTACTGGG; AGATTTGCAGTACCACACTTTTGGTCTTTAAGCCTGACCTGTTAGACAACCATCCAAGGC; TGGTGACTTTACGGAAGNAAGTCCAAGCCATTCCTGCCGTCGCTAACTGGATAAAACGAA; GGCCCCAAACCAAACTCTAGCTGATCCATGTTGCCCTCAAGTTTTGTTTTCTTCGGGGCA; TCTCTCTTATCAGATAGACAGCTACATCAGCCTGCCAGATATCCACATGCTCCCTCCCCA; GCGTCACTAAGATTTTCACTTTTACCATATTCTGATTTTTAAAAGGAAAAAAAAACAAAT; CTTTTTTAGTAAAAAAAAAAAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_014485
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014485.2, NP_055300.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 600 bp
• Locus ID: 27306
• UniProt ID: O60760
• Cytogenetics: 4q22.3
• Domains: GST_C, GST_N
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Summary: Prostaglandin-D synthase is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells. The presence of this enzyme can be used to identify the differentiation stage of human megakaryocytes. [provided by RefSeq, Jul 2008]