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TRPC4 (NM_016179) Human Untagged Clone

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SKU
SC114411
TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha
  $1,343.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TRPC4
Synonyms HTRP-4; HTRP4; TRP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC114411 representing NM_016179.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTA
AGAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCC
AGTGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTC
GGAAGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTT
AATGTCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTA
TTGAACCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAA
TTCACTCCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTG
GTTCAGAAAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGT
TCAGATGTGGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCT
CTCATTGCACTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTG
AGCAAGGTGGAAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAG
GACCTACTGGATCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGT
CTCATAGAAGAACAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAG
TTTGTTGCCCAGCCCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGG
AGAAGACACTGGGCAGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTG
TGCTACCTGATAGCTCCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGC
CACACAGCCTCCTATTTGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGAC
TTGAACAGGCAAGGTCCACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATA
TGGGGAGAAATTAAACAGATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATG
GACTTTGTAATGAACTCCTTATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGT
GCCCTTAATCCACGAGAATCATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATT
GCAAACATCTTCAGTTCTCTGCGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAA
ATATCTCTGGGAAGAATGCTCCTGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCA
TTTGCAAATGGCCTAAATCAATTGTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATA
AGATGTGAAAAGCAGAATAATGCATTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATA
TTTGGGCTCATCAATTTATATGTGACCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCC
ACCATGTTTGGGACATACAATGTCATCTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAAT
AATTCTTACCAACTGATTGCTGACCATGCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATG
AGTTATTTTGAAGAAGGAGGTACTCTGCCTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGG
TACCTGATCAAATGGATCTGGACACACTTGTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGA
ACAATAGGGAGGCGAGCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTG
GTGAAGCGATACGTTGCTGCAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTT
AAGGAACTAAAGCAAGACATTTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTT
TCCACAATACAATCTGCGAATGCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGAT
AGCGAAGGTAATAGCAAGGACAAGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATTCATCCG
AGATCAGCAGCAATTGCCTCTGAAAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCG
CCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAGACGA
TCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAA
CAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACCTG
AGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGGG
TTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATA
ATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACA
GTCACCCACGAAGATTACGTGACCACAAGATTGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_016179
Insert Size 2934 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016179.2
RefSeq Size 3534 bp
RefSeq ORF 2934 bp
Locus ID 7223
UniProt ID Q9UBN4
Cytogenetics 13q13.3
Domains ANK, ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW 112.1 kDa
Summary This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Aug 2011
Transcript Variant: This variant (1, also known as alpha) encodes isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
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"NM_016179" in other vectors (6)
SKU Description Size Price
RC218941 TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha 10 ug
$1,341.00
RC218941L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged 10 ug
$1,641.00
RC218941L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged 10 ug
$1,641.00
RC218941L3 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged 10 ug
$1,641.00
RC218941L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged 10 ug
$1,641.00
RG218941 TRPC4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha 10 ug
$1,541.00

file-alt Citations

Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells
Zhang, Dai, Cui et al
Biochim. Biophys. Acta (2014) 1853 (2), 377-387 DOI: 10.1016/j.bbamcr.2014.10.030
Cited by 30
AI Summary: OriGene’s pCMV6-XL4-TRPC4 (SC114411) and pCMV6-XL4 plasmids were used in this study to overexpress TRPC4 in vascular endothelial cells, enabling the authors to demonstrate TRPC4’s role in regulating autophagy.
Snippet: medium. The effect of gene silencing was monitored by western blot analysis. 2.9. Plasmid transfection. The pCMV6-XL4-TRPC4 (SC114411) and pCMV6-XL4 plasmids were purchased from Origene Technologies. The cells ... Cite SaveSaving
Showing 1-1 of 1 papers.
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