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TRPC4 (NM_016179) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG218941
TRPC4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,541.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TRPC4 or NM_016179"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRPC4
Synonyms HTRP-4; HTRP4; TRP4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG218941 representing NM_016179
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTGCGTCTGA
TCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCCTGGACAT
TTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATTGTACTTC
TATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCATTTTCAA
CGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGACCAATGT
CAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACANNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNATCACCAATACCAAGAAGTTATGAGGAACCTGGTGAAGCGATACGTTGCTG
CAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGACAT
TTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCGAAT
GCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGGTAATAGCAAGGACA
AGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATTCATCCGAGATCAGCAGCAATTGCCTCTGA
AAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCGCCCAGGGAGAAGCAGAGAAAAGTG
AATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAGACGATCAAAACAAAATGCTGCTGAGCAAA
ATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAACAGGCTGCAGGACCACTTGAGAGAAA
TATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAATGT
GTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTCCAT
TCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGAAGA
GGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTACGTGACCACAAGA
TTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG218941 representing NM_016179
Red=Cloning site Green=Tags(s)

MWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYF
YYEETKGLTCKGIRCEKQNNAFSTLFETLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTXXXXXX
XXXXXXXHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEENFKELKQDISSFRFEVLGLLRGSKLSTIQSAN
ASKESSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKV
NFVTDIKNFGLFHRRSKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQC
VLVDHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTR
L

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016179
ORF Size 1263 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016179.1, NP_057263.1
RefSeq Size 3440 bp
RefSeq ORF 2934 bp
Locus ID 7223
UniProt ID Q9UBN4
Cytogenetics 13q13.3
Domains ANK, ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Summary This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Aug 2011
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"NM_016179" in other vectors (6)
SKU Description Size Price
RC218941 TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha 10 ug
$1,341.00
RC218941L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged 10 ug
$1,641.00
RC218941L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged 10 ug
$1,641.00
RC218941L3 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged 10 ug
$1,641.00
RC218941L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged 10 ug
$1,641.00
SC114411 TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha 10 ug
$1,343.00

Plasmid Map for RG218941

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG218941
Circular map for RG218941

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