COQ2 (NM_015697) Human Untagged Clone

SKU SC112582

COQ2 (untagged)-Human coenzyme Q2 homolog, prenyltransferase (yeast) (COQ2), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: COQ2
• Synonyms: CL640; COQ10D1; MSA1; PHB:PPT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015697, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACCCCAATTTCACAAGTAAGGATGAGGAAAGGTTCTGCCCACACCGCCGCCCAGCCTGGCAGACTAG; GATTGCATCCTGCGGGTGCCACTGCGCATGCCTGCCGGGGAATGACGTCAATCCGAGCTCGTCCCGGCCT; CACCAGCGCCATGCTGGGCTCGCGAGCCGCGGGGTTCGCGCGGGGCCTGCGGGCTGTGGCACTGGCGTGG; CTGCCGGGCTGGCGGGGCCGCTCCTTCGCCCTGGCGCGTGCGGCAGGCGCGCCCCACGGTGGTGACTTGC; AGCCCCCCGCCTGTCCCGAGCCGCGCGGGCGCCAGCTCAGTTTGTCCGCGGCGGCGGTGGTGGACTCTGC; GCCCCGCCCCCTGCAGCCGTACTTGCGCCTCATGCGGTTGGACAAGCCCATTGGAACCTGGCTTCTGTAT; TTACCATGTACCTGGAGCATTGGTTTGGCAGCTGAACCAGGTTGTTTTCCAGATTGGTACATGCTCTCCC; TCTTTGGCACTGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCAGGACTA; TGATAAAAAGGTTACAAGAACAGCCAATCGTCCAATAGCCGCTGGAGACATTTCAACTTTTCAGTCCTTT; GTTTTTCTTGGGGGACAGCTAACCCTGGCACTGGGTGTTCTTCTGTGTCTAAATTACTACAGTATAGCTC; TGGGAGCAGGATCCTTACTTCTTGTCATCACCTACCCACTAATGAAAAGAATTTCATACTGGCCTCAACT; AGCCTTGGGCTTGACATTTAATTGGGGAGCGTTACTTGGATGGTCTGCTATCAAGGGTTCCTGTGATCCA; TCTGTTTGCCTGCCTCTTTATTTTTCTGGAGTTATGTGGACACTAATATATGATACTATTTATGCCCATC; AGGACAAAAGAGATGATGTTTTGATTGGTCTTAAGTCAACGGCTCTGCGGTTCGGAGAAAATACCAAGCC; GTGGCTCAGCGGCTTCAGTGTTGCAATGCTGGGGGCACTGAGCCTAGTGGGTGTGAACAGTGGACAGACT; GCTCCCTACTACGCTGCCCTGGGTGCTGTAGGAGCCCATCTGACTCACCAGATTTACACTCTAGACATCC; ACAGACCTGAGGATTGTTGGAATAAATTTATCTCCAACCGAACACTGGGACTAATAGTTTTTTTAGGGAT; TGTCCTTGGGAATTTGTGGAAAGAAAAGAAGACAGACAAAACAAAGAAGGGTATAGAGAATAAAATAGAA; AATTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015697 unedited; GGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGGGCTCGC; GAGCCGCGGGGTTCGCGCGGGGCCTGCGGGCTTTGGCACTGGCGTGGCTGCCGGGCTGGC; GGGGCCGCTCCTTCGCCCTGGCGCGTGCGGCAGGCGCGCCCCACGGTGGTGACTTGCAGC; CCCCCGCCTGTCCCGAGCCGCGCGGGCGCCAGCTCAGTTTGTCCGCGGCGGCGGTGGTGG; ACTCTGCGCCCCGCCCCCTGCAGCCGTACTTGCGCCTCATGCGGTTGGACAAGCCCATTG; GAACCTGGCTTCTGTATTTACCATGTACCTGGAGCATTGGTTTGGCAGCTGAACCAGGTT; GTTTTCCAGATTGGTACATGCTCTCCCTCTTTGGCACTGGAGCTATTCTGATGCGTGGAG; CAGGCTGTACTATTAATGACATGTGGGACCAGGACTATGATAAAAAGGTTACAAGAACAG; CCAATCGTCCAATAGCCGCTGGAGACATTTCAACTTTTCAGTCCTTTGTTTTTCTTGGGG; GACAGCTAACCCTGGCACTGGGTGTTCTTCTGTGTCTAAATTACTACAGTATAGCTCTGG; GAGCAGGATCCTTACTTCTTGTCATCACCTACCCACTAATGAAAAGAATTTCATACTGGC; CTCAACTAGCCTTGGGCTTGACATTTAATTGGGGAGCGTTACTTGGATGGTCTGCTATCA; AGGGTTCCTGTGATCCATCTGTTTGCCTGCCTCTTTATTTTTCTGGAGTTATGTGGACAC; TAATATATGACACTATTTATGCCCATCANGACANAAGAGATGATGTTTTGATTGGTCTTA; AGTCACGGCTCTGCNNGTTCGAGAAAATACAAGCCGTGGCTCAGCGGCTTCAGTGNNTGC; ATGCTGGGGGCACTGAGCCCTAGTGGTGTGAACAGTGGGAAGACTGCTNCCTACTACGCT; GCCCTGGGGTGCTGTAGACCCATCTGACTCACAGATTTACCTCTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_015697
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015697.5, NP_056512.3
• RefSeq Size: 1657 bp
• RefSeq ORF: 1266 bp
• Locus ID: 27235
• Cytogenetics: 4q21.22-q21.23
• Protein Families: Transmembrane
• Protein Pathways: Ubiquinone and other terpenoid-quinone biosynthesis
• Summary: This gene encodes an enzyme that functions in the final steps in the biosynthesis of CoQ (ubiquinone), a redox carrier in the mitochondrial respiratory chain and a lipid-soluble antioxidant. This enzyme, which is part of the coenzyme Q10 pathway, catalyzes the prenylation of parahydroxybenzoate with an all-trans polyprenyl group. Mutations in this gene cause coenzyme Q10 deficiency, a mitochondrial encephalomyopathy, and also COQ2 nephropathy, an inherited form of mitochondriopathy with primary renal involvement. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.