UBE2D2 (NM_003339) Human Untagged Clone

SKU SC111700

UBE2D2 (untagged)-Human ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: UBE2D2
• Synonyms: E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4; UBCH5B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111700 sequence for NM_003339 edited (data generated by NextGen Sequencing); ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAG; TGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCA; AATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTAC; CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT; AATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCA; AAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTG; CCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAA; TGGACTCAGAAGTATGCGATGTAA; ; Clone variation with respect to NM_003339.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003339 unedited; TGTATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCTGGGAGGAAGAGGCAG; CTACGGCGGCGGCGGCGGTGGCGGCTAGGGCGGCGGCGAATAAAGGGGCCGCCGCCGGGT; GATGCGGTGACCGCTGCGGCAGGCCCAGGAGCTGAGTGGGCCCCGGCCCTCAGCCCGTCC; CGCCGGACCCGCTTTCCTCAACTCTCCATCTTCTCCTGCCGACCGAGATCGCCGAGGCGG; CCTCAGGCTCCCTAGCCCCTTCCCCGTCCCTTCCCCGCCCCCGTCCCCGCCCCGGGGGCC; GCCGCCACCCGCCTCCCACCATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGG; CACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGC; AAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAA; TTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTT; ATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGT; CTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGGTGTGATCCC; AATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAG; TACAACAGAATAGCTCGGGGATGGACTCANAAGTATGCGATGTAATTAAAGAAATTATTG; GATAACCTCTACAATAAAGATAGGGGGACTCTGANAGAGAAAGTCCTTTTGATTTCATTT; GACTGCTTTCTATGAGCCACGCCTCATCTTNCCCTGTGCACATGTTACTGATACAGAGGG; GNTGGCGTGGTGGACTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003339 unedited; ACTTCCAGGGCCGGAAAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC; AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTATAT; GTAATGACTGTAGTAACCAGTTTATTACACAGATTAATCATTCTTGAAAGTACAAGCTCC; AGAGGAGCAATTGGGTCTTTAAATATACACAAGTATTTCCATCAAATGAATTTTCACCCT; TACATCCAAATAGACCTAAGCGGTTAAAAACATAAGAAAAATAAGAGCTATTAGCTATGT; ATTAACTGAGAAACCACATACAAACCAAAGAATATGGAAGAGAGAAAGAAGGGCTGAAAG; GCCAAAGGTTGAAGGGGTAGGGAGATGTAAAAGAAGTTGGGAACAAAGCCCATCACACTT; GATGTACTAATAGCTCCAATCCATTTAAATGTTGACCAGTTAAACTTAGACCTTAAAATG; CAGGATGTGGGTAGAGTTTAATCTGGTTGGTCATAACTTTCACCTAAGACGTAGCTCCTT; CGGAATAAAATGAATACAGACACAGCTTCATGTTCTTCACCTTGCTTTTCATAACTGTTT; TGAGTTTAAATGGATTCCACTTAAAAATAATCTCCTATAGGTGAGTGGAGCCTTTTCTTT; TCCATCCAAGCACACACCATACCCACCACCCCCCTTACACCTTGCTGNTATCCAAATTTA; AGTCAATAAGAAAAGCTTTTAGTCTCACACCTAGGTAATGCCTGTAGAACTGGCTTGTCA; CACACACACTTCTCTGCTNAGAGGCNAATGTTGTACAAAAGTACAGTATTTCTAGTTTTG; TGTTCAAGTATGTACACACGCAGCACTGCTGTATCAGGTAACATGTGCCAGGGGAAGATG; ANGCGTGGCTCATAAAAGCAGTCAATGAAATCAAAGGACTTNCTTTTCAAAGTCCCTTNC; TTATTTGGAAAAGGTTNCAATAATNTCTTATAACTCGCATCTTCTGAGACC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003339
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003339.2, NP_003330.1
• RefSeq Size: 2715 bp
• RefSeq ORF: 444 bp
• Locus ID: 7322
• UniProt ID: P62837
• Cytogenetics: 5q31.2
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Summary: Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]; Transcript Variant: This variant (1) encodes the longer isoform (1).