SNRPF (NM_003095) Human Untagged Clone

SKU SC108475

SNRPF (untagged)-Human small nuclear ribonucleoprotein polypeptide F (SNRPF)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SNRPF
• Synonyms: Sm-F; SMF; snRNP-F
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108475 sequence for NM_003095 edited (data generated by NextGen Sequencing); ATGAGTTTACCCCTCAATCCCAAACCTTTCCTCAATGGACTAACAGGAAAGCCAGTGATG; GTGAAACTTAAGTGGGGAATGGAGTACAAGGGCTATCTGGTATCTGTAGATGGCTACATG; AACATGCAGCTTGCAAATACAGAAGAATACATAGATGGAGCTTTGTCTGGACATCTGGGT; GAAGTTTTAATAAGGTGTAATAATGTCCTTTATATCAGAGGTGTGGAAGAAGAGGAAGAA; GATGGGGAAATGAGAGAATAG; ; Clone variation with respect to NM_003095.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003095 unedited; TTCGGCACCAGCCTGCGGTACCTGCTGTAGTCACGAGGGACGGGCGGCGGCCTGGTCGGC; AGAGAGTAGCCTGCAACATTCGGCCGTGGTTACGATGAGTTTACCCCTCAATCCCAAACC; TTTCCTCAATGGACTAACAGGAAAGCCAGTGATGGTGAAACTTAAGTGGGGAATGGAGTA; CAAGGGCTATCTGGTATCTGTAGATGGCTACATGAACATGCAGCTTGCAAATACAGAAGA; ATACATAGATGGAGCTTTGTCTGGACATCTGGGTGAAGTTTTAATAAGGTGTAATAATGT; CCTTTATATCAGAGGTGTGGAAGAAGAGGAAGAAGATGGGGAAATGAGAGAATAGCATCT; TTTGTGGGGGATTTTTTTTATATATATTTCTAGACAATAAAGATTTGTTTGTTTTTCAAA; ANAAAAAAAAAAAAAAAAAAAAAAAACCTCGCCTCTAGATTGCGGCCGCGGTCATAGCTG; TTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCC; TGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTT; TGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGG; GCAAGTTGGGAGAACACCTGTAGGGCCTGCGGNGTCTATTGGGAACCAAGCTGAGTGCAG; TGGCACAATCTTGGCTCACTGCAATCTCCCGCTNNCTGGGTCAAAGCGATTCTCTGCCTC; AGCCTCCCGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003095 unedited; TCTATGAACCGCGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTGAAAAACAAACAAATCTTTATTGGCTAGAAATATATATAAAAAAAATCCCCCACAAA; AGATGCTATTCTCTCATTTCCCCATCTTCTTCCTCTTCTTCCACACCTCTGATATAAAGG; ACATTATTACACCTTATTAAAACTTCACCCAGATGTCCAGACAAAGCTCCATCTATGTAT; TCTTCTGTATTTGCAAGCTGCATGTTCATGTAGCCATCTACAGATACCAGATAGCCCTTG; TACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTTTCCTGTTAGTCCATTGAGGAAA; GGTTTGGGATTGAGGGGTAAACTCATCGTAACCACGGCCGAATGTTGCAGGCTACTCTCT; GCCGACCAGGCCGCCGCCCGTCCCTCGTGACTACAGCAGGTACCGCAGGCTGGTGCCGAA; TTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGGTCACTAAACGAGCTC; TGCTTATATAGACCTCGCACCGTACACGCCTACCGCCCATTTGCGGTAACGGGGCGGGGT; TATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCCAAACAAACCCCCCTTGACGC; CAATGGGGGGGAGACTTGCAAATCCCCGGGAGGCGAACCGCTGTCCCCGCCGCATGGTGA; GCCGCCGAAAACGGCTCGCCCTGGCCATAGCCGAGACCAGAGCCGGAAATGGCTGCGTAT; AGNGAAACCCACCCCGCGCGCTGATCTGACAGTATGACCGGAGGGACAGCTAGCCAAGGA; TACTAAGTCACGGGGGGACCCGTATGCAGCCAACAGCACGAGCGCCGCCCGCNGGACACG; TACCGCCAGAGAACCCCACTCTACGCCGGTACCTAACCGCACGNACCAGCAAGTGCGGCG; CAGGCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003095
• Insert Size: 470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003095.1, NP_003086.1
• RefSeq Size: 462 bp
• RefSeq ORF: 261 bp
• Locus ID: 6636
• UniProt ID: P62306
• Cytogenetics: 12q23.1
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• Summary: Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:23333303, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed:12975319).[UniProtKB/Swiss-Prot Function]