Drd5 (NM_012768) Rat Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
|---|---|
| Target Symbol | Drd5 |
| Synonyms | D1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012768 |
| ORF Size | 1425 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012768.1, NP_036900.1 |
| RefSeq Size | 1428 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 25195 |
| UniProt ID | P25115 |
| Cytogenetics | 14q21 |
| MW | 52.8 kDa |
| Summary | dopamine receptor; may play a role in stimulation of adenylyl cyclase, activation of phospholipase C, and phosphatidylinositol phosphate metabolism in the central nervous system RGD, Feb 2006 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012768" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RN203152 | Drd5 (untagged ORF) - Rat dopamine receptor D5 (Drd5), (10 ug) | 10 ug |
$686.00
|
|
| RR203152L3 | Lenti ORF clone of Drd5 (Myc-DDK-tagged ORF) - Rat dopamine receptor D5 (Drd5), (10 ug) | 10 ug |
$986.00
|
|
| RR203152L4 | Lenti ORF clone of Drd5 (mGFP-tagged ORF) - Rat dopamine receptor D5 (Drd5), (10 ug) | 10 ug |
$986.00
|
|
Citations
Dopamine D1-like Receptors Regulate Constitutive, μ-Opioid Receptor-Mediated Repression of Use-Dependent Synaptic Plasticity in Dorsal Horn Neurons: More Harm than Good?
Aira, Barrenetxea, Buesa et al
J. Neurosci. (2016) 36 (20), 5661-73 DOI: 10.1523/JNEUROSCI.2469-15.2016
Aira, Barrenetxea, Buesa et al
J. Neurosci. (2016) 36 (20), 5661-73 DOI: 10.1523/JNEUROSCI.2469-15.2016
AI Summary: OriGene’s pCMV6 plasmids encoding C-terminal myc-tagged rat D1R and D5R were used to transfect HEK293T cells, enabling the authors to study dopamine D1-like receptor expression and function through immunofluorescence and Western blot analyses.
Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from the American Type Culture Collection (ATCC;CRL-3216) were maintained in DMEM (ATCC, 30-2002), supplemented with 10% fetal bovine serum (Sigma-Aldrich) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin;Invitrogen, Life Technologies). Before transfection, cells were plated onto poly-D-lysine-coated 18 mm glass coverslips (for fl...
Showing 1-1 of 1 papers.
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