Pparg (NM_001145366) Rat Tagged ORF Clone

  • TrueORF®
SKU
RR200399
Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug)
$289.00 $503.00
In Stock*
Specifications
Specifications
Product Data
Type Rat Tagged ORF Clone
Target Symbol Pparg
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145366
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145366.1, NP_001138838.1
RefSeq Size 1830 bp
RefSeq ORF 1428 bp
Locus ID 25664
UniProt ID O88275
Cytogenetics 4q42
MW 54.5 kDa
Summary ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism RGD, Feb 2006
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"NM_001145366" in other vectors (5)
SKU Description Size Price
RN200399 Pparg (untagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$503.00
RR200399L1 Lenti ORF clone of Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L2 Lenti ORF clone of Pparg (mGFP-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L3 Lenti ORF clone of Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L4 Lenti ORF clone of Pparg (mGFP-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00

Plasmid Map for RR200399

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Cloning scheme for RR200399

Circular map for RR200399

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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