Pparg (NM_001145366) Rat Untagged Clone

SKU
RN200399
Pparg (untagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug)
  $503.00
4 Weeks*
Specifications
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Pparg
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN200399 representing NM_001145366
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTCTGTGGACCTCTCTGTGATGG
ATGACCACTCCCATTCCTTTGACATCAAACCCTTTACCACGGTTGATTTCTCCAGCATTTCTGCTCCACA
CTATGAAGACATCCCGTTCACAAGAGCTGACCCAATGGTTGCTGATTACAAATATGACCTGAAGCTCCAA
GAATACCAAAGTGCGATCAAAGTAGAGCCTGCGTCCCCGCCTTATTATTCTGAAAAAACCCAACTCTACA
ACAGGCCACATGAAGAGCCTTCAAACTCCCTCATGGCCATCGAGTGCCGAGTCTGTGGGGATAAAGCATC
AGGCTTCCACTATGGAGTCCATGCTTGTGAAGGATGCAAGGGTTTTTTCCGAAGAACCATCCGATTGAAG
CTTATTTATGATAGGTGTGATCTTAACTGTCGGATCCACAAAAAGAGTAGAAATAAATGTCAGTACTGTC
GGTTTCAGAAGTGCCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTTGGGCGAATGCCACAGGCCGA
GAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACCCAGAGTCTGCTGATCTGCGA
GCCCTGGCAAAGCATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCCAAGGCGAGGGCGA
TCTTGACAGGAAAGACAACAGACAAATCACCATTTGTCATCTACGACATGAATTCCTTAATGATGGGAGA
AGACAAAATCAAGTTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGCATTTTT
CAAGGGTGCCAGTTTCGATCCGTGGAAGCTGTGCAAGAGATCACAGAGTATGCCAAAAATATCCCTGGTT
TCATTAACCTTGACTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTCCATGAGATCATCTACACCAT
GCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATATCAGAGGGACAAGGATTCATGACCAGGGAGTTC
CTCAAAAGCCTGCGGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGAGTTTGCTGTGAAGTTCAATG
CACTGGAATTAGATGACAGTGACTTGGCCATATTTATAGCTGTCATTATTCTCAGTGGAGACCGCCCAGG
CTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGGCCCTGGAACTCCAGCTGAAG
CTGAACCACCCGGAGTCCTCCCAGCTGTTCGCCAAGGTGCTCCAGAAGATGACAGACCTCAGGCAGATTG
TCACAGAGCACGTGCAGCTACTGCATGTGATCAAGAAGACGGAGACAGATATGAGCCTTCACCCTCTGCT
CCAGGAGATCTACAAGGACTTGTATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001145366
Insert Size 1428 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145366.1, NP_001138838.1
RefSeq Size 1830 bp
RefSeq ORF 1428 bp
Locus ID 25664
UniProt ID O88275
Cytogenetics 4q42
Summary ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism RGD, Feb 2006
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. Both variants 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001145366" in other vectors (5)
SKU Description Size Price
RR200399 Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$289.00 $503.00
RR200399L1 Lenti ORF clone of Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L2 Lenti ORF clone of Pparg (mGFP-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L3 Lenti ORF clone of Pparg (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00
RR200399L4 Lenti ORF clone of Pparg (mGFP-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 2, (10 ug) 10 ug
$803.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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