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APOPT1 (NM_032374) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG229120
APOPT1 (tGFP-tagged) - Human chromosome 14 open reading frame 153 (C14orf153)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "APOPT1 or NM_032374"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol APOPT1
Synonyms APOP; APOP1; C14orf153
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG229120 representing NM_032374
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCGTGCGCCGCGGGAGCCAGGGGGCGTGGGGCCATGGTGGTCTTGCGGGCGGGGAAGAAGACCT
TTCTCCCCCCTCTCTGCCGCGCCTTCGCCTGCCGCGGCTGTCAACTCGCTCCGGAGCGCGGCGCCGAGCG
CAGGGATACGGCGCCCAGCGGGGTCTCAAGATTCTGCCCTCCAAGAAAGTCTTGCCATGATTGGATAGGA
CCCCCAGATAAATATTCAAACCTTCGACCTGTTCACTTTTACATACCTGAAAATGAATCTCCATTGGAAC
AAAAGCTTAGAAAATTAAGACAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAACCAGAATTTGAC
TTTTAGTAAGGAAAAAGAAGAATTTATTCACTCAAGACTAAAAACTAAAGGCCTGGGCCTGAGAACTGAA
TCAGGTCAGAAAGCAACATTGAATGCAGAAGAAATGGCGGACTTCTACAAGGAATTTTTAAGTAAAAATT
TTCAGAAGCACATGTATTATAACAGAGATTGGTACAAGCGCAATTTTGCCATCACCTTCTTCATGGGAAA
AGTGGCCCTGGAAAGGATTTGGAACAAGCTTAAACAGAAACAAAAGAAGAGGAGCAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG229120 representing NM_032374
Red=Cloning site Green=Tags(s)

MLPCAAGARGRGAMVVLRAGKKTFLPPLCRAFACRGCQLAPERGAERRDTAPSGVSRFCPPRKSCHDWIG
PPDKYSNLRPVHFYIPENESPLEQKLRKLRQETQEWNQQFWANQNLTFSKEKEEFIHSRLKTKGLGLRTE
SGQKATLNAEEMADFYKEFLSKNFQKHMYYNRDWYKRNFAITFFMGKVALERIWNKLKQKQKKRSN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032374
ORF Size 618 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032374.4, NP_115750.2
RefSeq Size 1244 bp
RefSeq ORF 621 bp
Locus ID 84334
UniProt ID Q96IL0
Cytogenetics 14q32.33
Protein Families Secreted Protein
Summary This gene encodes a protein that localizes to the mitochondria, where it stimulates the release of cytochrome c, thereby promoting programmed cell death. Mutations in this gene have been found in individuals with mitochondrial complex IV deficiency. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014
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"NM_032374" in other vectors (5)
SKU Description Size Price
RC229120 APOPT1 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$300.00
RC229120L3 Lenti ORF clone of Human chromosome 14 open reading frame 153 (C14orf153), Myc-DDK-tagged 10 ug
$600.00
RC229120L4 Lenti ORF clone of Human chromosome 14 open reading frame 153 (C14orf153), mGFP tagged 10 ug
$600.00
SC108011 APOPT1 (untagged)-Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$300.00
SC327755 APOPT1 (untagged)-Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$330.00

Plasmid Map for RG229120

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG229120
Circular map for RG229120

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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