APOPT1 (NM_032374) Human Untagged Clone

SKU
SC327755
APOPT1 (untagged)-Human chromosome 14 open reading frame 153 (C14orf153)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol APOPT1
Synonyms APOP; APOP1; C14orf153
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327755 representing NM_032374.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGCCGTGCGCCGCGGGAGCCAGGGGGCGTGGGGCCATGGTGGTCTTGCGGGCGGGGAAGAAGACC
TTTCTCCCCCCTCTCTGCCGCGCCTTCGCCTGCCGCGGCTGTCAACTCGCTCCGGAGCGCGGCGCCGAG
CGCAGGGATACGGCGCCCAGCGGGGTCTCAAGATTCTGCCCTCCAAGAAAGTCTTGCCATGATTGGATA
GGACCCCCAGATAAATATTCAAACCTTCGACCTGTTCACTTTTACATACCTGAAAATGAATCTCCATTG
GAACAAAAGCTTAGAAAATTAAGACAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAACCAGAAT
TTGACTTTTAGTAAGGAAAAAGAAGAATTTATTCACTCAAGACTAAAAACTAAAGGCCTGGGCCTGAGA
ACTGAATCAGGTCAGAAAGCAACATTGAATGCAGAAGAAATGGCGGACTTCTACAAGGAATTTTTAAGT
AAAAATTTTCAGAAGCACATGTATTATAACAGAGATTGGTACAAGCGCAATTTTGCCATCACCTTCTTC
ATGGGAAAAGTGGCCCTGGAAAGGATTTGGAACAAGCTTAAACAGAAACAAAAGAAGAGGAGCAACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_032374
Insert Size 621 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032374.4
RefSeq Size 2528 bp
RefSeq ORF 621 bp
Locus ID 84334
UniProt ID Q96IL0
Cytogenetics 14q32.33
Protein Families Secreted Protein
MW 24.2 kDa
Summary This gene encodes a protein that localizes to the mitochondria, where it stimulates the release of cytochrome c, thereby promoting programmed cell death. Mutations in this gene have been found in individuals with mitochondrial complex IV deficiency. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_032374" in other vectors (5)
SKU Description Size Price
RC229120 APOPT1 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$300.00
RC229120L3 Lenti ORF clone of Human chromosome 14 open reading frame 153 (C14orf153), Myc-DDK-tagged 10 ug
$600.00
RC229120L4 Lenti ORF clone of Human chromosome 14 open reading frame 153 (C14orf153), mGFP tagged 10 ug
$600.00
RG229120 APOPT1 (tGFP-tagged) - Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$500.00
SC108011 APOPT1 (untagged)-Human chromosome 14 open reading frame 153 (C14orf153) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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