Apolipoprotein L 1 (APOL1) (NM_001136540) Human Tagged ORF Clone
$657.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Apolipoprotein L 1 |
| Synonyms | APO-L; APOL; APOL-I; FSGS4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001136540 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001136540.1, NP_001130012.1 |
| RefSeq Size | 2924 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 8542 |
| UniProt ID | O14791 |
| Cytogenetics | 22q12.3 |
| Protein Families | Secreted Protein, Transmembrane |
| Summary | This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001136540" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC226566 | APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 3 | 10 ug |
$457.00
|
|
| RC226566L3 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 3, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC226566L4 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 3, mGFP tagged | 10 ug |
$757.00
|
|
| SC325047 | APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 3 | 10 ug |
$503.00
|
|
Citations
A novel APOL1 membrane-addressing domain mutation (p.T272I) in Chinese twins with FSGS: implications for podocyte injury and ion channel disruption
Peng, Chang, Chen et al
Ren Fail (2025) 47 (1), 2553384 DOI: 10.1080/0886022X.2025.2553384
Peng, Chang, Chen et al
Ren Fail (2025) 47 (1), 2553384 DOI: 10.1080/0886022X.2025.2553384
AI Summary: OriGene's pCMV6-entry vector encoding APOL1 cDNA (catalog number RG226566) was used to express wild-type and mutant APOL1 in cell models, enabling functional studies of the novel APOL1 mutation's effects on podocyte injury and ion channel disruption in FSGS.
Snippet: … pCMV6-entry vector encoding the transcript variant 3 of APOL1 cDNA (RefSeqORF: 1197 bp) with C-terminal MYC and DDK tags was purchased from OriGene (RG226566). The APOL1 new variants were generated using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies, Cat. 200524), Forward primer: 5′-CCTTAGCTGGCAATATTTACCAACTCACAC;- pCMV6-entry vector encoding the transcript variant 3 of APOL1 cDNA (RefSeqORF: 1197 bp) with C-terminal MYC and DDK tags was purchased from OriGene (RG226566
Blocking the 5' splice site of exon 4 by a morpholino oligomer triggers APOL1 protein isoform switch
Cheatham, Davis, Khatua et al
Sci Rep (2018) 8 (1), 8739 DOI: 10.1038/s41598-018-27104-x
Cheatham, Davis, Khatua et al
Sci Rep (2018) 8 (1), 8739 DOI: 10.1038/s41598-018-27104-x
AI Summary: OriGene’s APOL1 cDNA (splice variant A, NM_003661) in the pCMV6-XL5 vector was used to express APOL1 and generate deletion mutants, enabling the study of exon 4 splicing and isoform switching in this paper.
Snippet: ) and transfected with 2 μg of total DNA per well using jetPrime reagent (VWR). APOL1 expression vectors APOL1 cDNA corresponding to splice variant A (NM_003661) was obtained in pCMV6-XL5 vector (OriGene). The open reading frame of APOL1 genes expressed from pCMV6-XL5 was modified by PCR with myc epitope24. APOL1 deletion mutants were created using the QuickChange II site-directed mutagenesis
The innate immune factor apolipoprotein L1 (APOL1) restricts HIV-1 infection
Taylor, Khatua, Popik
J. Virol. (2013)
Taylor, Khatua, Popik
J. Virol. (2013)
Showing 1-3 of 3 papers.
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