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FLRT2 (NM_013231) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG224270
FLRT2 (tGFP-tagged) - Human fibronectin leucine rich transmembrane protein 2 (FLRT2)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $814.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLRT2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG224270 representing NM_013231
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTACAGACCACAAAGTGGCCCAGCCATGGGGCTTTTTTCCTGAAGTCTTGGCTTATCATTTCCC
TGGGGCTCTACTCACAGGTGTCCAAACTCCTGGCCTGCCCTAGTGTGTGCCGCTGCGACAGGAACTTTGT
CTACTGTAATGAGCGAAGCTTGACCTCAGTGCCTCTTGGGATCCCGGAGGGCGTAACCGTACTCTACCTC
CACAACAACCAAATTAATAATGCTGGATTTCCTGCAGAACTGCACAATGTACAGTCGGTGCACACGGTCT
ACCTGTATGGCAACCAACTGGACGAATTCCCCATGAACCTTCCCAAGAATGTCAGAGTTCTCCATTTGCA
GGAAAACAATATTCAGACCATTTCACGGGCTGCTCTTGCCCAGCTCTTGAAGCTTGAAGAGCTGCACCTG
GATGACAACTCCATATCCACAGTGGGGGTGGAAGACGGGGCCTTCCGGGAGGCTATTAGCCTCAAATTGT
TGTTTTTGTCTAAGAATCACCTGAGCAGTGTGCCTGTTGGGCTTCCTGTGGACTTGCAAGAGCTGAGAGT
GGATGAAAATCGAATTGCTGTCATATCCGACATGGCCTTCCAGAATCTCACGAGCTTGGAGCGTCTTATT
GTGGACGGGAACCTCCTGACCAACAAGGGTATCGCCGAGGGCACCTTCAGCCATCTCACCAAGCTCAAGG
AATTTTCAATTGTACGTAATTCGCTGTCCCACCCTCCTCCCGATCTCCCAGGTACGCATCTGATCAGGCT
CTATTTGCAGGACAACCAGATAAACCACATTCCTTTGACAGCCTTCTCAAATCTGCGTAAGCTGGAACGG
CTGGATATATCCAACAACCAACTGCGGATGCTGACTCAAGGGGTTTTTGATAATCTCTCCAACCTGAAGC
AGCTCACTGCTCGGAATAACCCTTGGTTTTGTGACTGCAGTATTAAATGGGTCACAGAATGGCTCAAATA
TATCCCTTCATCTCTCAACGTGCGGGGTTTCATGTGCCAAGGTCCTGAACAAGTCCGGGGGATGGCCGTC
AGGGAATTAAATATGAATCTTTTGTCCTGTCCCACCACGACCCCCGGCCTGCCTCTCTTCACCCCAGCCC
CAAGTACAGCTTCTCCGACCACTCAGCCTCCCACCCTCTCTATTCCAAACCCTAGCAGAAGCTACACGCC
TCCAACTCCTACCACATCGAAACTTCCCACGATTCCTGACTGGGATGGCAGAGAAAGAGTGACCCCACCT
ATTTCTGAACGGATCCAGCTCTCTATCCATTTTGTGAATGATACTTCCATTCAAGTCAGCTGGCTCTCTC
TCTTCACCGTGATGGCATACAAACTCACATGGGTGAAAATGGGCCACAGTTTAGTAGGGGGCATCGTTCA
GGAGCGCATAGTCAGCGGTGAGAAGCAACACCTGAGCCTGGTTAACTTAGAGCCCCGATCCACCTATCGG
ATTTGTTTAGTGCCACTGGATGCTTTTAACTACCGCGCGGTAGAAGACACCATTTGTTCAGAGGCCACCA
CCCATGCCTCCTATCTGAACAACGGCAGCAACACAGCGTCCAGCCATGAGCAGACGACGTCCCACAGCAT
GGGCTCCCCCTTTCTGCTGGCGGGCTTGATCGGGGGCGCGGTGATATTTGTGCTGGTGGTCTTGCTCAGC
GTCTTTTGCTGGCATATGCACAAAAAGGGGCGCTACACCTCCCAGAAGTGGAAATACAACCGGGGCCGGC
GGAAAGATGATTATTGCGAGGCAGGCACCAAGAAGGACAACTCCATCCTGGAGATGACAGAAACCAGTTT
TCAGATCGTCTCCTTAAATAACGATCAACTCCTTAAAGGAGATTTCAGACTGCAGCCCATTTACACCCCA
AATGGGGGCATTAATTACACAGACTGCCATATCCCCAACAACATGCGATACTGCAACAGCAGCGTGCCAG
ACCTGGAGCACTGCCATACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG224270 representing NM_013231
Red=Cloning site Green=Tags(s)

MGLQTTKWPSHGAFFLKSWLIISLGLYSQVSKLLACPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYL
HNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHL
DDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLI
VDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFSNLRKLER
LDISNNQLRMLTQGVFDNLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAV
RELNMNLLSCPTTTPGLPLFTPAPSTASPTTQPPTLSIPNPSRSYTPPTPTTSKLPTIPDWDGRERVTPP
ISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYR
ICLVPLDAFNYRAVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGSPFLLAGLIGGAVIFVLVVLLS
VFCWHMHKKGRYTSQKWKYNRGRRKDDYCEAGTKKDNSILEMTETSFQIVSLNNDQLLKGDFRLQPIYTP
NGGINYTDCHIPNNMRYCNSSVPDLEHCHT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013231
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013231.5
RefSeq Size 7185 bp
RefSeq ORF 1983 bp
Locus ID 23768
UniProt ID O43155
Cytogenetics 14q31.3
Domains FN3, LRR, LRRCT, LRRNT, LRR_TYP
Protein Families Druggable Genome, Transmembrane
Summary This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2016
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"NM_013231" in other vectors (6)
SKU Description Size Price
RC224270 FLRT2 (Myc-DDK-tagged)-Human fibronectin leucine rich transmembrane protein 2 (FLRT2) 10 ug
$614.00
RC224270L1 Lenti ORF clone of Human fibronectin leucine rich transmembrane protein 2 (FLRT2), Myc-DDK-tagged 10 ug
$914.00
RC224270L2 Lenti ORF clone of Human fibronectin leucine rich transmembrane protein 2 (FLRT2), mGFP tagged 10 ug
$914.00
RC224270L3 Lenti ORF clone of Human fibronectin leucine rich transmembrane protein 2 (FLRT2), Myc-DDK-tagged 10 ug
$914.00
RC224270L4 Lenti ORF clone of Human fibronectin leucine rich transmembrane protein 2 (FLRT2), mGFP tagged 10 ug
$914.00
SC115333 FLRT2 (untagged)-Human fibronectin leucine rich transmembrane protein 2 (FLRT2) 10 ug
$615.00

Plasmid Map for RG224270

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG224270
Circular map for RG224270

file-alt Citations

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