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NOL6 (NM_139235) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG224100
NOL6 (tGFP-tagged) - Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $840.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOL6
Synonyms bA311H10.1; NRAP; UTP22
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG224100 representing NM_139235
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCGGCGCCAGCTGGAGAGCAGCTTCGCGGAGCGACTGGAGAGCCAGAGGTGATGGAACCAGCCC
TGGAAGGCACAGGCAAAGAGGGGAAGAAAGCATCCTCCAGGAAGCGTACATTGGCTGAACCTCCAGCGAA
GGGCCTCCTGCAGCCAGTGAAGCTCAGCAGGGCAGAACTGTACAAGGAGCCTACCAATGAGGAGCTTAAT
CGCCTTCGGGAGACTGAGATCTTGTTCCACTCCAGCTTGCTTCGTTTACAGGTAGAGGAGCTACTAAAGG
AAGTAAGGCTGTCAGAGAAGAAGAAGGATCGGATTGATGCCTTCCTACGGGAGGTCAACCAGCGGGTTGT
GAGGGTGCCCTCAGTCCCTGAGACAGAGCTCACTGACCAGGCATGGCTCCCAGCTGGGGTTCGAGTGCCC
CTCCACCAAGTGCCCTATGCCGTGAAGGGCTGTTTCCGCTTCCTGCCCCCAGCCCAGGTTACTGTTGTGG
GCAGCTACCTTCTGGGCACCTGCATCCGACCAGACATCAATGTGGATGTGGCACTGACCATGCCCAGGGA
AATCCTACAGGACAAGGACGGGCTGAACCAGCGCTACTTCCGCAAGCGTGCCCTCTACCTGGCCCACTTG
GCTCACCACCTGGCCCAGGACCCCCTCTTTGGCAGTGTTTGCTTCTCCTACACAAATGGCTGCCACCTGA
AACCCTCACTGTTGCTGCGGCCGCGTGGAAAGGATGAGCGCCTGGTCACTGTACGTCTGCATCCGTGCCC
TCCACCTGACTTCTTCCGCCCGTGCCGCTTGCTGCCAACCAAGAACAATGTGCGCTCTGCCTGGTACCGA
GGGCAGAGTCCTGCAGGGGATGGTAGCCCAGAGCCTCCTACCCCCCGCTATAACACATGGGTCCTGCAAG
ATACAGTTCTCGAGTCCCATTTGCAGCTGCTGTCAACCATTCTGAGTTCAGCCCAGGGCCTGAAGGATGG
CGTGGCACTTCTGAAGGTCTGGCTGCGGCAGCGGGAGCTGGACAAGGGCCAGGGTGGGTTTACTGGGTTC
CTTGTCTCCATGCTGGTTGTCTTCCTTGTGTCTACACGCAAGATCCATACCACCATGAGTGGCTACCAGG
TCCTGAGAAGTGTCTTGCAGTTTCTGGCCACTACAGACCTGACAGTCAACGGGATCAGTTTATGTCTCAG
CTCAGATCCCTCTTTGCCGGCCCTGGCTGACTTCCACCAGGCCTTCTCCGTTGTCTTCCTGGATTCCTCA
GGCCATCTCAACCTCTGTGCTGATGTCACTGCCTCTACTTACCACCAGGTACAGCATGAGGCACGGCTGT
CTATGATGTTGCTGGACAGCAGAGCTGACGACGGGTTCCACCTGCTGTTGATGACTCCCAAACCCATGAT
CCGGGCTTTTGACCATGTCCTGCATCTCCGTCCACTGAGTCGCCTGCAGGCAGCGTGCCACCGGCTGAAG
CTCTGGCCAGAGCTGCAGGACAATGGTGGGGACTATGTCTCAGCTGCTTTGGGCCCCCTGACCACCCTCC
TGGAGCAGGGCCTGGGGGCTCGGCTGAACCTGCTGGCTCACTCTCGACCCCCAGTCCCAGAGTGGGACAT
CAGCCAAGATCCACCAAAGCACAAAGACTCTGGGACCCTGACCCTGGGACTCCTTCTCCGGCCTGAGGGA
CTGACCAGCGTCCTTGAGCTGGGTCCAGAGGCAGACCAGCCTGAGGCTGCTAAATTCCGCCAGTTCTGGG
GATCCCGCTCGGAGCTTCGGCGTTTCCAGGACGGAGCCATTCGGGAAGCTGTGGTCTGGGAGGCAGCCTC
TATGTCCCAGAAGCGCCTTATTCCCCACCAGGTGGTCACCCACCTCTTGGCACTCCATGCTGACATCCCA
GAAACCTGTGTCCACTATGTGGGGGGCCCCCTGGATGCACTTATCCAAGGCCTGAAAGAGGCCTCCAGCA
CAAAGGGGCGCATGGTGATGTCTCGAGGTGGGGAGCTAGTAATGGTGCCCAATGTTGAAGCAATCCTGGA
GGACTTTGCTGTGCTGGGTGAAGGCCTGGTGCAGACTGTGGAGGCCCGAAGTGAGAGGTGGACTGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG224100 representing NM_139235
Red=Cloning site Green=Tags(s)

MGPAPAGEQLRGATGEPEVMEPALEGTGKEGKKASSRKRTLAEPPAKGLLQPVKLSRAELYKEPTNEELN
RLRETEILFHSSLLRLQVEELLKEVRLSEKKKDRIDAFLREVNQRVVRVPSVPETELTDQAWLPAGVRVP
LHQVPYAVKGCFRFLPPAQVTVVGSYLLGTCIRPDINVDVALTMPREILQDKDGLNQRYFRKRALYLAHL
AHHLAQDPLFGSVCFSYTNGCHLKPSLLLRPRGKDERLVTVRLHPCPPPDFFRPCRLLPTKNNVRSAWYR
GQSPAGDGSPEPPTPRYNTWVLQDTVLESHLQLLSTILSSAQGLKDGVALLKVWLRQRELDKGQGGFTGF
LVSMLVVFLVSTRKIHTTMSGYQVLRSVLQFLATTDLTVNGISLCLSSDPSLPALADFHQAFSVVFLDSS
GHLNLCADVTASTYHQVQHEARLSMMLLDSRADDGFHLLLMTPKPMIRAFDHVLHLRPLSRLQAACHRLK
LWPELQDNGGDYVSAALGPLTTLLEQGLGARLNLLAHSRPPVPEWDISQDPPKHKDSGTLTLGLLLRPEG
LTSVLELGPEADQPEAAKFRQFWGSRSELRRFQDGAIREAVVWEAASMSQKRLIPHQVVTHLLALHADIP
ETCVHYVGGPLDALIQGLKEASSTKGRMVMSRGGELVMVPNVEAILEDFAVLGEGLVQTVEARSERWTV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139235
ORF Size 2097 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139235.3, NP_631981.2
RefSeq Size 3554 bp
RefSeq ORF 2100 bp
Locus ID 65083
UniProt ID Q9H6R4
Cytogenetics 9p13.3
Protein Families Druggable Genome
Summary The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. provided by RefSeq, Jul 2008
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"NM_139235" in other vectors (4)
SKU Description Size Price
RC224100 NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$640.00
RC224100L3 Lenti-ORF clone of NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$940.00
RC224100L4 Lenti-ORF clone of NOL6 (mGFP-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$940.00
SC314464 NOL6 (untagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$705.00

Plasmid Map for RG224100

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG224100
Circular map for RG224100

file-alt Citations

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