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NOL6 (NM_139235) Human Untagged Clone

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SKU
SC314464
NOL6 (untagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma
  $705.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NOL6
Synonyms bA311H10.1; NRAP; UTP22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC314464 representing NM_139235.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGGCCGGCGCCAGCTGGAGAGCAGCTTCGCGGAGCGACTGGAGAGCCAGAGGTGATGGAACCAGCC
CTGGAAGGCACAGGCAAAGAGGGGAAGAAAGCATCCTCCAGGAAGCGTACATTGGCTGAACCTCCAGCG
AAGGGCCTCCTGCAGCCAGTGAAGCTCAGCAGGGCAGAACTGTACAAGGAGCCTACCAATGAGGAGCTT
AATCGCCTTCGGGAGACTGAGATCTTGTTCCACTCCAGCTTGCTTCGTTTACAGGTAGAGGAGCTACTA
AAGGAAGTAAGGCTGTCAGAGAAGAAGAAGGATCGGATTGATGCCTTCCTACGGGAGGTCAACCAGCGG
GTTGTGAGGGTGCCCTCAGTCCCTGAGACAGAGCTCACTGACCAGGCATGGCTCCCAGCTGGGGTTCGA
GTGCCCCTCCACCAAGTGCCCTATGCCGTGAAGGGCTGTTTCCGCTTCCTGCCCCCAGCCCAGGTTACT
GTTGTGGGCAGCTACCTTCTGGGCACCTGCATCCGACCAGACATCAATGTGGATGTGGCACTGACCATG
CCCAGGGAAATCCTACAGGACAAGGACGGGCTGAACCAGCGCTACTTCCGCAAGCGTGCCCTCTACCTG
GCCCACTTGGCTCACCACCTGGCCCAGGACCCCCTCTTTGGCAGTGTTTGCTTCTCCTACACAAATGGC
TGCCACCTGAAACCCTCACTGTTGCTGCGGCCGCGTGGAAAGGATGAGCGCCTGGTCACTGTACGTCTG
CATCCGTGCCCTCCACCTGACTTCTTCCGCCCGTGCCGCTTGCTGCCAACCAAGAACAATGTGCGCTCT
GCCTGGTACCGAGGGCAGAGTCCTGCAGGGGATGGTAGCCCAGAGCCTCCTACCCCCCGCTATAACACA
TGGGTCCTGCAAGATACAGTTCTCGAGTCCCATTTGCAGCTGCTGTCAACCATTCTGAGTTCAGCCCAG
GGCCTGAAGGATGGCGTGGCACTTCTGAAGGTCTGGCTGCGGCAGCGGGAGCTGGACAAGGGCCAGGGT
GGGTTTACTGGGTTCCTTGTCTCCATGCTGGTTGTCTTCCTTGTGTCTACACGCAAGATCCATACCACC
ATGAGTGGCTACCAGGTCCTGAGAAGTGTCTTGCAGTTTCTGGCCACTACAGACCTGACAGTCAACGGG
ATCAGTTTATGTCTCAGCTCAGATCCCTCTTTGCCGGCCCTGGCTGACTTCCACCAGGCCTTCTCCGTT
GTCTTCCTGGATTCCTCAGGCCATCTCAACCTCTGTGCTGATGTCACTGCCTCTACTTACCACCAGGTA
CAGCATGAGGCACGGCTGTCTATGATGTTGCTGGACAGCAGAGCTGACGACGGGTTCCACCTGCTGTTG
ATGACTCCCAAACCCATGATCCGGGCTTTTGACCATGTCCTGCATCTCCGTCCACTGAGTCGCCTGCAG
GCAGCGTGCCACCGGCTGAAGCTCTGGCCAGAGCTGCAGGACAATGGTGGGGACTATGTCTCAGCTGCT
TTGGGCCCCCTGACCACCCTCCTGGAGCAGGGCCTGGGGGCTCGGCTGAACCTGCTGGCTCACTCTCGA
CCCCCAGTCCCAGAGTGGGACATCAGCCAAGATCCACCAAAGCACAAAGACTCTGGGACCCTGACCCTG
GGACTCCTTCTCCGGCCTGAGGGACTGACCAGCGTCCTTGAGCTGGGTCCAGAGGCAGACCAGCCTGAG
GCTGCTAAATTCCGCCAGTTCTGGGGATCCCGCTCGGAGCTTCGGCGTTTCCAGGACGGAGCCATTCGG
GAAGCTGTGGTCTGGGAGGCAGCCTCTATGTCCCAGAAGCGCCTTATTCCCCACCAGGTGGTCACCCAC
CTCTTGGCACTCCATGCTGACATCCCAGAAACCTGTGTCCACTATGTGGGGGGCCCCCTGGATGCACTT
ATCCAAGGCCTGAAAGAGGCCTCCAGCACAAAGGGGCGCATGGTGATGTCTCGAGGTGGGGAGCTAGTA
ATGGTGCCCAATGTTGAAGCAATCCTGGAGGACTTTGCTGTGCTGGGTGAAGGCCTGGTGCAGACTGTG
GAGGCCCGAAGTGAGAGGTGGACTGTGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_139235
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139235.3
RefSeq Size 3554 bp
RefSeq ORF 2100 bp
Locus ID 65083
UniProt ID Q9H6R4
Cytogenetics 9p13.3
Protein Families Druggable Genome
MW 77.6 kDa
Summary The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (gamma) lacks nine exons in the 3' region compared to variant alpha. The encoded isoform (gamma), also known as the short isoform, has a unique C-terminus compared to the alpha isoform.
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Documents
Resources
"NM_139235" in other vectors (4)
SKU Description Size Price
RC224100 NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$640.00
RC224100L3 Lenti-ORF clone of NOL6 (Myc-DDK-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$940.00
RC224100L4 Lenti-ORF clone of NOL6 (mGFP-tagged)-Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$940.00
RG224100 NOL6 (tGFP-tagged) - Human nucleolar protein family 6 (RNA-associated) (NOL6), transcript variant gamma 10 ug
$840.00

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