TLE3 (NM_005078) Human Tagged ORF Clone

SKU RG224066

TLE3 (tGFP-tagged) - Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (TLE3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224066 representing NM_005078; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTG; AGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA; CGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC; TTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGC; CTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGAC; GGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCC; CCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCT; CCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCA; TGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCC; AGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACA; GCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCC; CGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAA; AAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACC; TTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCC; AACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATA; GGTATAATGGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGA; TGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACC; CCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCT; GTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAG; GAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGC; CCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGT; GCCGTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACA; TCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTC; CTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGAC; CTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCA; TTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAA; CCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGC; ACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTAC; AGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGG; CATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAG; AGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTC; TCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTG; TGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTC; ATCTAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224066 representing NM_005078; Red=Cloning site Green=Tags(s); ; MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG; LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGP; PVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRA; SEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLK; KDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI; GIMASALRTPISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSPMVSFGA; VGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVC; AVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWD; LASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDG; TKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHE; SCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEV; IY; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005078
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005078.4
• RefSeq Size: 5354 bp
• RefSeq ORF: 2319 bp
• Locus ID: 7090
• UniProt ID: Q04726
• Cytogenetics: 15q23
• Domains: TLE_N, WD40
• Protein Families: Transcription Factors
• Summary: This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]