TLE3 (NM_005078) Human Untagged Clone

SKU SC116972

TLE3 (untagged)-Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (TLE3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005078, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTG; AGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA; CGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC; TTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGC; CTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGAC; GGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCC; CCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCT; CCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCA; TGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCC; AGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACA; GCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCC; CGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAA; AAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACC; TTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCC; AACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATA; GGTATAATGGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGA; TGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACC; CCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCT; GTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAG; GAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGC; CCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGT; GCCGTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACA; TCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTC; CTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGAC; CTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCA; TTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAA; CCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGC; ACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTAC; AGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGG; CATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAG; AGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTC; TCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTG; TGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTC; ATCTACTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005078 unedited; CCATTTGTAAACGACTTATATAGGCGGCCGCGCAATTCGCACCAGTCCACGCTCTCGGAA; TCACGACCCCTCCCTGCCATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGG; CAGCCGGGATTTAAATTCACGGTGGCTGAGTCTTGTGACAGGATCAAAGACGAATTCCAG; TTCCTGCAAGCTCAGTATCACAGCCTCATAGTGGAGTACGACAAGCTGGCAAACGAGAAG; ACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAACATTGAA; ATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGCCT; TTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTC; ACCATGACGGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCC; CATGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCA; GGAATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCGCCCTGNGCAGC; CAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAGA; GAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCAC; CGGNGCTCTGCGGACTACAGCATGGAAGCCAAGAAAGCGAAGGCNGAGGAGAAGGACAGC; TTGAGCCGATACGACAGTGATGGGGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAAA; TGAAGACCCGCACGCCCCGGGTCAGCCCGGCACACTCCCTCCCTGAAATGGGCTGGNACA; GGCCNGTAGCCTGAANAAAAGATGCCCCACAGCCTGGCTCGNTGGNNCTCTTCAGTAGCA; CACCTTTCTNNCAGACAAAGACCTGGTCATN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005078 unedited; GGACAACTTATGGACGCGGCCGCATTCTTAAGATCGATTTTTTTTTTTTTTTTTTTTTTT; TTTGGAATTTCCGGGGGGAAGGGGCCTCCTTTCCCATTGTTAATTCCCGAATTAGAAGTC; GGTTTTTAACATTTTTAAAAAATTTTGGGCCCTGAAAAATCGGAAAAAAAAAACCCCCCC; ATCCCCAAGAAAAAATTTGGGGTCCATTCCAGCACCCCCAACCCAAGGGGAGGGACAGGG; CAAAAAAAAAGGGAAAAACAACCAAAACCTCTTGACCGGGTTTGGGGGGAAACCGAACCC; TTAACCCTCCAATAAATTTTTTTGTACCACCTGCCACCATTGTTAAGGGGGAGGAGGAGA; ACCAAAACCCTTCCAAGTCTGGGAAACACATAATTCCAGGCCCAGAATTCCAAACTGGGA; CGGGGAACGTGCCCTTTAAGCCGGCCGAAGCGAACCCCTGAACCCTGGGTTGCCTGGGGC; CAATCCTCCGCCATCCTGGGGGCCCCTCCCCTGGGGGTCTCCCTGTAAAACCCAATTCGG; TTTTTCCCAAATTTTGACAGCCCTGCTGAAGTTCTGGTTAATTAAATAACCTCTTAAACG; GGGGCCTTCTTGTAACCAAAGCCGGTTACAAGGAATTGGTAATCCGCTGAAATGTCCAAC; TCAGAACGAACAAGGATTCTTAAAACTGGATATATGCTGCTCCATAAAGCGTCTTCCAGC; CGTTGAAAAGGTATCTTTTCCATTGCTCACAAACCACTTGCCGCATTAGGCGAACTGTGA; GGAAAGCACCCCACTTTTCTGCAGGTCCAACTGGGACTTGTCAGGCCTGGTGTGGGGGCA; CAACCTCCAAGTTGGTGGTTTTCATGCCCAAAGGCAGCCATTCCCAGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005078
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005078.1, NP_005069.1
• RefSeq Size: 5760 bp
• RefSeq ORF: 5760 bp
• Locus ID: 7090
• UniProt ID: Q04726
• Cytogenetics: 15q23
• Domains: TLE_N, WD40
• Protein Families: Transcription Factors
• Summary: This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.