EOMES (NM_005442) Human Tagged ORF Clone

SKU RG221014

EOMES (tGFP-tagged) - Human eomesodermin (EOMES)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: EOMES
• Synonyms: TBR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221014 representing NM_005442; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAGTTAGGGGAGCAGCTCTTGGTGAGCTCAGTGAACCTGCCTGGCGCGCACTTCTACCCGCTGGAGA; GTGCGCGAGGCGGCAGCGGCGGGAGCGCTGGCCACCTCCCCAGCGCGGCCCCCTCTCCTCAGAAGTTGGA; CTTAGACAAAGCGTCCAAGAAGTTTTCCGGCAGTCTCTCCTGCGAGGCGGTGAGCGGGGAGCCCGCAGCC; GCCAGCGCAGGGGCCCCCGCGGCCATGCTTAGTGACACCGACGCCGGGGACGCATTTGCCAGCGCTGCGG; CAGTGGCCAAGCCGGGGCCCCCGGACGGCCGCAAGGGCTCCCCCTGCGGGGAGGAGGAGCTGCCCTCCGC; CGCTGCAGCCGCCGCCGCCGCCGCCGCCGCGGCTGCGGCCACTGCGCGCTACTCCATGGACAGCCTGAGC; TCCGAGCGGTACTACCTCCAGTCCCCCGGTCCTCAGGGGTCGGAGCTGGCTGCGCCCTGCTCACTCTTCC; CGTACCAGGCGGCGGCTGGGGCGCCCCACGGACCTGTGTACCCGGCTCCTAACGGGGCGCGCTACCCCTA; CGGCTCCATGCTGCCCCCCGGCGGCTTCCCCGCGGCTGTGTGCCCACCCGGGAGGGCGCAGTTCGGCCCA; GGAGCCGGTGCGGGCAGTGGCGCGGGCGGTAGCAGCGGCGGGGGCGGCGGCCCGGGCACCTATCAGTACA; GCCAGGGGGCTCCGCTCTACGGGCCGTACCCTGGAGCCGCAGCGGCGGGATCTTGCGGAGGACTGGGGGG; CCTGGGGGTTCCAGGTTCTGGCTTCCGTGCCCACGTCTACCTGTGCAACCGGCCTCTGTGGCTCAAATTC; CACCGCCACCAAACTGAGATGATCATTACGAAACAGGGCAGGCGCATGTTTCCTTTCTTGAGCTTCAACA; TAAACGGACTCAATCCCACTGCCCACTACAATGTGTTCGTAGAGGTGGTGCTGGCGGACCCCAACCACTG; GCGCTTCCAGGGGGGCAAATGGGTGACCTGTGGCAAAGCCGACAATAACATGCAGGGCAACAAAATGTAT; GTTCACCCAGAGTCTCCTAATACTGGTTCCCACTGGATGAGACAGGAGATTTCATTCGGGAAATTAAAAC; TCACCAATAACAAAGGCGCAAATAACAACAACACCCAGATGATAGTCTTACAATCCTTACACAAATACCA; ACCCCGACTGCATATTGTTGAAGTTACAGAGGATGGCGTGGAGGACTTGAATGAGCCCTCAAAGACCCAG; ACTTTTACCTTCTCAGAAACGCAATTCATTGCAGTGACTGCCTACCAAAACACCGATATTACTCAACTAA; AGATTGATCATAACCCCTTTGCAAAAGGCTTCAGAGACAACTATGATTCATCCCATCAGATTGTCCCTGG; AGGTCGGTACGGCGTTCAATCCTTCTTCCCGGAGCCCTTTGTCAACACTTTACCTCAAGCCCGCTATTAT; AATGGCGAGAGAACCGTGCCACAGACCAACGGCCTCCTTTCACCCCAACAGAGCGAAGAGGTGGCCAACC; CTCCCCAGCGGTGGCTTGTCACGCCTGTCCAGCAACCTGGGACCAACAAACTAGACATCAGTTCCTATGA; ATCTGAATATACTTCTAGCACATTGCTCCCATATGGCATTAAATCCTTGCCCCTTCAGACATCCCATGCC; CTGGGGTATTACCCAGACCCAACCTTTCCTGCAATGGCAGGGTGGGGAGGTCGAGGTTCTTACCAGAGGA; AGATGGCAGCTGGACTACCATGGACCTCCAGAACAAGCCCCACTGTGTTCTCTGAAGATCAGCTCTCCAA; GGAGAAAGTGAAAGAGGAAATTGGCTCTTCTTGGATAGAGACACCCCCTTCCATCAAATCTCTAGATTCC; AATGATTCAGGAGTATACACCAGTGCTTGTAAGCGAAGGCGGCTGTCTCCTAGCAACTCCAGTAATGAAA; ATTCACCCTCCATAAAGTGTGAGGACATTAATGCTGAAGAGTATAGTAAAGACACCTCAAAAGGCATGGG; AGGGTATTATGCTTTTTACACAACTCCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221014 representing NM_005442; Red=Cloning site Green=Tags(s); ; MQLGEQLLVSSVNLPGAHFYPLESARGGSGGSAGHLPSAAPSPQKLDLDKASKKFSGSLSCEAVSGEPAA; ASAGAPAAMLSDTDAGDAFASAAAVAKPGPPDGRKGSPCGEEELPSAAAAAAAAAAAAAATARYSMDSLS; SERYYLQSPGPQGSELAAPCSLFPYQAAAGAPHGPVYPAPNGARYPYGSMLPPGGFPAAVCPPGRAQFGP; GAGAGSGAGGSSGGGGGPGTYQYSQGAPLYGPYPGAAAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWLKF; HRHQTEMIITKQGRRMFPFLSFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNKMY; VHPESPNTGSHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSKTQ; TFTFSETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSSHQIVPGGRYGVQSFFPEPFVNTLPQARYY; NGERTVPQTNGLLSPQQSEEVANPPQRWLVTPVQQPGTNKLDISSYESEYTSSTLLPYGIKSLPLQTSHA; LGYYPDPTFPAMAGWGGRGSYQRKMAAGLPWTSRTSPTVFSEDQLSKEKVKEEIGSSWIETPPSIKSLDS; NDSGVYTSACKRRRLSPSNSSNENSPSIKCEDINAEEYSKDTSKGMGGYYAFYTTP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005442
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005442.4
• RefSeq Size: 2756 bp
• RefSeq ORF: 2061 bp
• Locus ID: 8320
• UniProt ID: O95936
• Cytogenetics: 3p24.1
• Domains: T-box
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Summary: This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]