GRAMD1B (NM_020716) Human Tagged ORF Clone

SKU RG219269

GRAMD1B (tGFP-tagged) - Human GRAM domain containing 1B (GRAMD1B)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GRAMD1B
• Synonyms: LINC01059
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219269 representing NM_020716; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAAGGATTCAAGCTCTCCTGCACTGCCAGTAACTCCAACCGCAGCACGCCGGCCTGCTCGCCCATCC; TCCGGAAGCGGTCTCGCTCGCCAACCCCGCAGAACCAGGACGGAGACACCATGGTGGAGAAGGGCTCAGA; TCACTCCTCGGACAAGTCCCCGTCCACACCGGAGCAGGGCGTGCAGCGCAGCTGCTCCTCCCAGTCCGGC; CGGAGCGGCGGCAAGAATTCCAAGAAAAGCCAGAGTTGGTATAATGTGTTAAGCCCCACCTACAAGCAGA; GAAATGAAGACTTCAGAAAGCTCTTTAAGCAGCTTCCAGACACGGAGCGCCTCATTGTTGATTACTCATG; TGCACTCCAAAGAGACATTCTCCTTCAGGGCCGACTCTACCTCTCTGAAAATTGGATCTGCTTCTACAGC; AACATCTTCCGCTGGGAAACTCTGCTGACAGTCCGTTTGAAAGACATCTGTTCCATGACTAAAGAAAAAA; CAGCTCGCCTCATTCCCAATGCCATCCAAGTTTGCACTGATTCAGAAAAGCACTTCTTCACTTCGTTTGG; GGCCCGGGATAGGACATATATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGAAAAGCCTCTGTGT; CCCAAGGAGCTCTGGCACTTTGTTCACCAGTGCTATGGGAACGAATTGGGCCTGACCAGTGATGACGAGG; ACTACGTGCCCCCTGACGACGACTTCAACACAATGGGATACTGTGAAGAGATCCCTGTGGAAGAGAATGA; AGTGAATGACAGCTCATCCAAGAGCAGCATAGAGACCAAGCCAGATGCCAGTCCACAGCTGCCCAAGAAA; TCCATCACCAACAGCACACTAACATCCACAGGGAGCAGTGAGGCCCCCGTCTCGTTTGATGGGCTGCCCC; TGGAGGAAGAGGCGCTGGAGGGAGACGGGTCCCTGGAAAAGGAGCTCGCCATTGACAACATCATGGGGGA; GAAGATTGAGATGATCGCTCCTGTGAACTCCCCTTCACTGGACTTCAATGACAATGAGGACATCCCCACT; GAGCTCAGTGACTCTTCCGACACACACGATGAAGGAGAGGTCCAGGCCTTCTATGAGGACCTGAGTGGCC; GGCAGTACGTGAATGAAGTCTTCAACTTCAGCGTGGACAAGCTCTATGACCTCCTCTTCACCAACTCGCC; CTTCCAGCGGGATTTCATGGAGCAGCGGCGCTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAG; AATGGAAACCAGAGCCGAGTGATTCTTTACACCATCACCCTTACCAACCCTCTGGCTCCCAAAACTGCCA; CTGTCAGGGAGACACAGACCATGTACAAGGCGAGCCAGGAGAGTGAATGTTACGTGATAGATGCCGAAGT; CCTCACCCACGACGTGCCCTACCACGACTACTTCTACACAATCAATCGCTACACGCTCACCCGTGTGGCT; CGGAACAAGAGCCGACTCAGGGTCTCCACAGAGCTGCGCTATCGAAAACAGCCCTGGGGGTTAGTGAAAA; CGTTCATCGAGAAGAACTTCTGGAGTGGGCTGGAGGACTACTTCCGCCATTTAGAGAGCGAGCTGGCCAA; AACGGAGAGCACTTATTTGGCTGAGATGCACAGACAATCTCCCAAAGAGAAGGCCAGCAAGACTACAACG; GTGCGGAGGAGGAAGCGTCCCCATGCCCACCTGCGAGTCCCTCACCTGGAAGAGGTGATGAGCCCGGTCA; CCACGCCCACAGATGAGGATGTGGGCCACAGGATCAAACATGTGGCAGGTTCCACACAGACGCGGCATAT; CCCGGAGGACACCCCCAACGGTTTCCACCTGCAGAGCGTGTCCAAGCTGCTGCTGGTTATCAGCTGTGTG; ATCTGTTTCAGTCTGGTGCTGCTGGTCATCCTTAACATGATGCTCTTCTACAAACTCTGGATGTTGGAAT; ACACCACGCAGACCCTCACTGCCTGGCAGGGTCTAAGGCTCCAAGAAAGGTTACCCCAGTCTCAGACAGA; ATGGGCCCAGCTCTTAGAGTCCCAACAAAAGTACCACGATACTGAGCTCCAAAAATGGAGGGAAATCATC; AAATCCTCAGTGATGCTCCTTGACCAGATGAAGGACTCGCTCATCAACCTTCAGAACGGCATCAGGTCCC; GCGACTACACGTCGGAAAGTGAAGAAAAGAGGAATCGCTATCAT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219269 representing NM_020716; Red=Cloning site Green=Tags(s); ; MKGFKLSCTASNSNRSTPACSPILRKRSRSPTPQNQDGDTMVEKGSDHSSDKSPSTPEQGVQRSCSSQSG; RSGGKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYS; NIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLC; PKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKK; SITNSTLTSTGSSEAPVSFDGLPLEEEALEGDGSLEKELAIDNIMGEKIEMIAPVNSPSLDFNDNEDIPT; ELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKEE; NGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVA; RNKSRLRVSTELRYRKQPWGLVKTFIEKNFWSGLEDYFRHLESELAKTESTYLAEMHRQSPKEKASKTTT; VRRRKRPHAHLRVPHLEEVMSPVTTPTDEDVGHRIKHVAGSTQTRHIPEDTPNGFHLQSVSKLLLVISCV; ICFSLVLLVILNMMLFYKLWMLEYTTQTLTAWQGLRLQERLPQSQTEWAQLLESQQKYHDTELQKWREII; KSSVMLLDQMKDSLINLQNGIRSRDYTSESEEKRNRYH; ; TRTRPLE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020716
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020716.1, NP_065767.1
• RefSeq Size: 7906 bp
• RefSeq ORF: 2217 bp
• Locus ID: 57476
• UniProt ID: Q3KR37
• Cytogenetics: 11q24.1
• Protein Families: Transmembrane
• Summary: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).[UniProtKB/Swiss-Prot Function]