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ZNF189 (NM_003452) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG217848
ZNF189 (tGFP-tagged) - Human zinc finger protein 189 (ZNF189), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $783.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF189
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG217848 representing NM_003452
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGGGTTGCTGACATTTGAGGATGTGGCTGTGTTTTTTA
CCCAGGAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTGTATAAAGATGTCATGATGGAGAATTA
TGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGATGAGGAGCCAACTGTAAAACAAGAGATT
GAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTTACAAGAATCAAAAGTGAAATTGACCAGG
ATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTAGATAAACAAAGAGGGATCTTCCTATGGGA
AATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTCAGAGAAAACACTAACATTATCCGTAAAAGA
CCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGTGGAAAGGGTTTTGTCCGCAAGGCCCATTTCA
TTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTTCAGTGCAATGAATGTGGGAAAAGTTTTAGTCG
CAGTTCATTTGTTATTGAACATCAGAGAATTCACACTGGGGAAAGGCCCTATGAGTGTAATTACTGTGGA
AAAACCTTTAGTGTGAGCTCAACCCTTATTAGACATCAGAGAATCCACACTGGAGAAAGACCCTATCAGT
GTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGGAGCCTTGTTAAACATCAAAGGATTCATACAGGTGA
GAAACCCCATAAATGTAGTGACTGTGGGAAAGCCTTCAGTTGGAAATCACACCTTATTGAGCATCAAAGA
ACTCACACTGGTGAGAAACCTTATCACTGTACCAAATGTAAGAAGAGCTTTAGTCGAAATTCATTGCTTG
TTGAGCATCAAAGAATTCACACTGGGGAAAGACCCCATAAATGTGGTGAATGTGGGAAAGCCTTTCGATT
AAGCACATACCTTATACAACACCAAAAAATTCACACTGGCGAGAAGCCTTTTCTTTGTATTGAGTGTGGA
AAAAGTTTCAGTCGGAGCTCATTCCTTATTGAACATCAGAGGATCCATACTGGTGAAAGACCTTATCAGT
GCAAAGAGTGTGGGAAAAGTTTCAGTCAGCTTTGCAACCTTACTCGTCATCAGAGAATTCACACAGGAGA
CAAGCCCCATAAATGTGAGGAATGTGGAAAAGCCTTTAGTAGAAGCTCAGGTCTTATTCAGCATCAGAGA
ATTCACACCAGGGAGAAGACTTATCCATACAATGAAACTAAGGAAAGTTTTGATCCAAATTGCAGTCTTG
TTATACAGCAGGAAGTCTACCCTAAGGAGAAATCTTATAAATGTGATGAATGTGGGAAAACTTTTAGTGT
TAGTGCTCATCTTGTACAACATCAAAGAATCCACACTGGTGAAAAGCCCTATCTATGTACTGTCTGTGGG
AAAAGCTTCAGCCGGAGCTCATTTCTTATTGAACATCAGAGAATCCACACTGGTGAGAGACCCTATCTGT
GCAGACAGTGTGGAAAAAGCTTTAGTCAGCTTTGTAATCTTATTCGACATCAGGGTGTTCACACAGGTAA
TAAACCCCATAAATGTGATGAATGTGGAAAGGCCTTTAGCCGGAACTCGGGTCTTATTCAGCATCAGAGA
ATACACACAGGAGAGAAACCTTATAAGTGTGAGAAGTGCGACAAAAGTTTCAGTCAACAGCGCAGTCTTG
TCAACCATCAGAAGATCCATGCAGAGGTGAAAACCCAAGAAACCCATGAATGTGACGCTTGTGGTGAAGC
CTTTAATTGCCGTATTTCTCTTATTCAGCATCAGAAATTGCACACAGCATGGATGCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG217848 representing NM_003452
Red=Cloning site Green=Tags(s)

MASPSPPPESKGLLTFEDVAVFFTQEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEI
EEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKR
PNSEEKCHKCEECGKGFVRKAHFIQHQRVHTGEKPFQCNECGKSFSRSSFVIEHQRIHTGERPYECNYCG
KTFSVSSTLIRHQRIHTGERPYQCNQCKQSFSQRRSLVKHQRIHTGEKPHKCSDCGKAFSWKSHLIEHQR
THTGEKPYHCTKCKKSFSRNSLLVEHQRIHTGERPHKCGECGKAFRLSTYLIQHQKIHTGEKPFLCIECG
KSFSRSSFLIEHQRIHTGERPYQCKECGKSFSQLCNLTRHQRIHTGDKPHKCEECGKAFSRSSGLIQHQR
IHTREKTYPYNETKESFDPNCSLVIQQEVYPKEKSYKCDECGKTFSVSAHLVQHQRIHTGEKPYLCTVCG
KSFSRSSFLIEHQRIHTGERPYLCRQCGKSFSQLCNLIRHQGVHTGNKPHKCDECGKAFSRNSGLIQHQR
IHTGEKPYKCEKCDKSFSQQRSLVNHQKIHAEVKTQETHECDACGEAFNCRISLIQHQKLHTAWMQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003452
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003452.4
RefSeq Size 3186 bp
RefSeq ORF 1881 bp
Locus ID 7743
UniProt ID O75820
Cytogenetics 9q31.1
Domains KRAB, zf-C2H2
Protein Families Transcription Factors
Summary Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 PubMed 9653648). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.supplied by OMIM, May 2010
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"NM_003452" in other vectors (4)
SKU Description Size Price
RC217848 ZNF189 (Myc-DDK-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 10 ug
$289.00 $583.00
RC217848L3 Lenti-ORF clone of ZNF189 (Myc-DDK-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 10 ug
$883.00
RC217848L4 Lenti-ORF clone of ZNF189 (mGFP-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 10 ug
$883.00
SC303308 ZNF189 (untagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1 10 ug
$642.00

Plasmid Map for RG217848

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG217848
Circular map for RG217848

file-alt Citations

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