ZNF189 (NM_003452) Human Untagged Clone

SKU SC303308

ZNF189 (untagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZNF189
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303308 representing NM_003452.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGGGTTGCTGACATTTGAGGATGTGGCTGTGTTTTTT; ACCCAGGAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTGTATAAAGATGTCATGATGGAGAAT; TATGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGATGAGGAGCCAACTGTAAAACAAGAG; ATTGAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTTACAAGAATCAAAAGTGAAATTGAC; CAGGATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTAGATAAACAAAGAGGGATCTTCCTA; TGGGAAATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTCAGAGAAAACACTAACATTATCCGT; AAAAGACCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGTGGAAAGGGTTTTGTCCGCAAGGCC; CATTTCATTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTTCAGTGCAATGAATGTGGGAAAAGT; TTTAGTCGCAGTTCATTTGTTATTGAACATCAGAGAATTCACACTGGGGAAAGGCCCTATGAGTGTAAT; TACTGTGGAAAAACCTTTAGTGTGAGCTCAACCCTTATTAGACATCAGAGAATCCACACTGGAGAAAGA; CCCTATCAGTGTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGGAGCCTTGTTAAACATCAAAGGATT; CATACAGGTGAGAAACCCCATAAATGTAGTGACTGTGGGAAAGCCTTCAGTTGGAAATCACACCTTATT; GAGCATCAAAGAACTCACACTGGTGAGAAACCTTATCACTGTACCAAATGTAAGAAGAGCTTTAGTCGA; AATTCATTGCTTGTTGAGCATCAAAGAATTCACACTGGGGAAAGACCCCATAAATGTGGTGAATGTGGG; AAAGCCTTTCGATTAAGCACATACCTTATACAACACCAAAAAATTCACACTGGCGAGAAGCCTTTTCTT; TGTATTGAGTGTGGAAAAAGTTTCAGTCGGAGCTCATTCCTTATTGAACATCAGAGGATCCATACTGGT; GAAAGACCTTATCAGTGCAAAGAGTGTGGGAAAAGTTTCAGTCAGCTTTGCAACCTTACTCGTCATCAG; AGAATTCACACAGGAGACAAGCCCCATAAATGTGAGGAATGTGGAAAAGCCTTTAGTAGAAGCTCAGGT; CTTATTCAGCATCAGAGAATTCACACCAGGGAGAAGACTTATCCATACAATGAAACTAAGGAAAGTTTT; GATCCAAATTGCAGTCTTGTTATACAGCAGGAAGTCTACCCTAAGGAGAAATCTTATAAATGTGATGAA; TGTGGGAAAACTTTTAGTGTTAGTGCTCATCTTGTACAACATCAAAGAATCCACACTGGTGAAAAGCCC; TATCTATGTACTGTCTGTGGGAAAAGCTTCAGCCGGAGCTCATTTCTTATTGAACATCAGAGAATCCAC; ACTGGTGAGAGACCCTATCTGTGCAGACAGTGTGGAAAAAGCTTTAGTCAGCTTTGTAATCTTATTCGA; CATCAGGGTGTTCACACAGGTAATAAACCCCATAAATGTGATGAATGTGGAAAGGCCTTTAGCCGGAAC; TCGGGTCTTATTCAGCATCAGAGAATACACACAGGAGAGAAACCTTATAAGTGTGAGAAGTGCGACAAA; AGTTTCAGTCAACAGCGCAGTCTTGTCAACCATCAGAAGATCCATGCAGAGGTGAAAACCCAAGAAACC; CATGAATGTGACGCTTGTGGTGAAGCCTTTAATTGCCGTATTTCTCTTATTCAGCATCAGAAATTGCAC; ACAGCATGGATGCAATAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_003452
• Insert Size: 1881 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003452.3
• RefSeq Size: 3213 bp
• RefSeq ORF: 1881 bp
• Locus ID: 7743
• UniProt ID: O75820
• Cytogenetics: 9q31.1
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 73 kDa
• Summary: Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]; Transcript Variant: This variant (1) encodes the longest isoform (1).