OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

MAP4K5 (NM_006575) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RG217191
MAP4K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $975.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA322481 Anti-MAP4K5 Rabbit Polyclonal Antibody
100 ul
$305.00
TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "MAP4K5 or NM_006575"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP4K5
Synonyms GCKR; KHS; KHS1; MAPKKKK5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG217191 representing NM_006575
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGGAACCCGCAGCAGGACTACGAACTCGTCC
AGAGGGTCGGCAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGC
AGTAAAAATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAA
GAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTA
TGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATGTTACTGGACCATTATCAGAATTGCAAAT
AGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAAGGCAAAATGCATAGAGAT
ATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTAAAATTAGCTGACTTTGGTGTGGCTGCAA
AAATAACAGCTACCATTGCAAAACGAAAATCTTTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGC
AGCAGTAGAGAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAA
CTTGGAGAACTTCAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA
ATTTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTCAAAATAGC
ACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCACACTTTTGTTGCACAGCCA
GGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTGAACAATCCAGATAACCACGCACATTACA
CTGAAGCAGATGACGATGACTTTGAGCCCCATGCAATCATTCGTCATACCATTAGATCTACAAACAGGAA
TGCCAGAGCTGAACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAA
GAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTG
TTGATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAG
GATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACCATAAAACATTGTCCTGAT
TCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTT
CTTCTATTGGAAATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACC
ACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCA
AAAGTTCTGATGGGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACAT
CCTGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTACACACTGAA
TCTCAATGAGCTACATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGTACTTGGCTGTATGTTATC
AATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAATCTTATAGCTTTGT
TTGAACATGCCAAAAAACCAGGATTAGCTGCCCATATTCAAACTCACAGGTTTCCAGACCGAATACTACC
AAGAAAATTCGCTTTAACAACAAAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAAC
CCTTACATGGGACATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGC
CAATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTTTTTGAAAT
GCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAAGGCACTGAATCGAATCAG
GTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCATGGTTTACAGAAATTGGTGCAGGCAGCC
AGCAGTTAGATTCCATTCATGTAACACAGTTGGAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGT
GAAAATTGTAAATCTACAAGGAAAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTT
CGCATTGAATCTGTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAA
GCTTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTAGGATCAGA
CAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGCAATCTCTACATCTTGGCT
GGACATGAAAATAGTTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG217191 representing NM_006575
Red=Cloning site Green=Tags(s)

MEAPLRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVK
ECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRD
IKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIE
LGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVAQP
GLSRALAVELLDKVNNPDNHAHYTEADDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRK
ETEARDEMGLSSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPD
SESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPAINGLPPTP
KVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVI
NNTLMSLSEGKTFQLYSHNLIALFEHAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRN
PYMGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQ
VVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDF
RIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILA
GHENSY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006575
ORF Size 2538 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006575.3, NP_006566.2
RefSeq Size 4373 bp
RefSeq ORF 2541 bp
Locus ID 11183
UniProt ID Q9Y4K4
Cytogenetics 14q22.1
Domains CNH, pkinase
Protein Families Druggable Genome, Protein Kinase
Summary This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_006575" in other vectors (7)
SKU Description Size Price
RC217191 MAP4K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$775.00
RC217191L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,075.00
RC217191L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged 10 ug
$1,075.00
RC217191L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,075.00
RC217191L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged 10 ug
$1,075.00
SC110237 MAP4K5 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$776.00
SC323591 MAP4K5 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$776.00

Plasmid Map for RG217191

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG217191
Circular map for RG217191

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products