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MAP4K5 (NM_006575) Human Untagged Clone

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SKU
SC323591
MAP4K5 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1
  $776.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAP4K5
Synonyms GCKR; KHS; KHS1; MAPKKKK5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_006575, the custom clone sequence may differ by one or more nucleotides


ATGGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGGAACCCGCAGCAGGACTACGAACTCGTCC
AGAGGGTCGGCAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGC
AGTAAAAATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAA
GAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTA
TGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATGTTACTGGACCATTATCAGAATTGCAAAT
AGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAAGGCAAAATGCATAGAGAT
ATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTAAAATTAGCTGACTTTGGTGTGGCTGCAA
AAATAACAGCTACCATTGCAAAACGAAAATCTTTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGC
AGCAGTAGAGAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAA
CTTGGAGAACTTCAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA
ATTTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTCAAAATAGC
ACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCACACTTTTGTTGCACAGCCA
GGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTGAACAATCCAGATAACCACGCACATTACA
CTGAAGCAGATGACGATGACTTTGAGCCCCATGCAATCATTCGTCATACCATTAGATCTACAAACAGGAA
TGCCAGAGCTGAACGGACAGCTTCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAA
GAAACAGAAGCACGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTG
TTGATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCTAAGCCAAG
GATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACCATAAAACATTGTCCTGAT
TCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGCCCAAGTTGTGGGCCTGTGGCAGAGACTT
CTTCTATTGGAAATGGTGATGGTATTTCAAAACTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACC
ACAGTTACCACGAAAAAAGGACAAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCA
AAAGTTCTGATGGGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACAT
CCTGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTACACACTGAA
TCTCAATGAGCTACATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGTACTTGGCTGTATGTTATC
AATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAATCTTATAGCTTTGT
TTGAACATGCCAAAAAACCAGGATTAGCTGCCCATATTCAAACTCACAGGTTTCCAGACCGAATACTACC
AAGAAAATTCGCTTTAACAACAAAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAAC
CCTTACACGGGACATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGC
CAATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTTTTTGAAAT
GCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAAGGCACTGAATCGAATCAG
GTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCATGGTTTACAGAAATTGGTGCAGGCAGCC
AGCAGTTAGATTCCATTCATGTAACACAGTTGGAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGT
GAAAATTGTAAATCTACAAGGAAAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTT
CGCATTGAATCTGTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAA
GCTTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTAGGATCAGA
CAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGCAATCTCTACATCTTGGCT
GGACATGAAAATAGTTACTAA

5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_006575 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGCGGCGACTGT
ACGCGCCTCCGCCGCCCCCGAGAGGACGCGCCGTGCAGCGGCTGAGTGGCGGCGGCGGCGACGGCAAACC
CGGAGCTGCCGGCCGGCGCGCGGGAGGAGGACGCGGGTGCGGTCTAGGAAACGGAGCTGCGGGCGGAGGC
TCCATGTTGGGAAGCGGCGCCGTTCGTGCTTGTTAGCGGGAATCCGGGAGCCGCGGGGTGAGCTGGCCGG
GGGGCCCGGGCCCTAAGTGAAGATGGAAGGCCCCCGCTGGCGGCCCTGCCGGCGGACATCCTGAGGCGGA
CCCCGCAGCAGGACTTACGACCTCGTCCAGGAGGGTCGGCACCGGCACCTAACGGGGACGTCCTATAAGG
CCGAAATGTACACACAGGAGAGCCTGGCTTGAATAATGAATCATTAAATTGAACCTGGAAGGTTATTTTC
TTGAATTACACAGAATTTTTATGGTAAGAATGTACCATTGAACATCGGTGCATCTTGGGGAGTACTTATC
GGAAACCTGGATGTAGGATACGGGGGGGTCTCAATTTCCAGTTCTGACATTCGATGCAATTCCTAGTTCG
AAACTTAAGGTCTGCAATTGCTCACGCACCGTGAATCAGGTCTTTTTTTGGCACTCGGCGATAATAACAA
TAGGTCAAATCCACTTGAAAAAATT
Kinase Domain Sequence
>SC323591 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ARTCAGAGGGTCGGAGCGGCACCTACGGGGACGTCTATAAGGCCAGAAATGTACACACAGGAGAGCTGGC
TGCAGTAATGATCATTAAATTGGAGCCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTT
AAAGAATGTAAACATTGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTT
GTATGGAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATG
Restriction Sites Please inquire |
ACCN NM_006575
Insert Size 4310 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006575.3, NP_006566.2
RefSeq Size 4373 bp
RefSeq ORF 2541 bp
Locus ID 11183
UniProt ID Q9Y4K4
Cytogenetics 14q22.1
Domains CNH, pkinase
Protein Families Druggable Genome, Protein Kinase
Summary This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.
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Documents
Resources
"NM_006575" in other vectors (7)
SKU Description Size Price
RC217191 MAP4K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$775.00
RC217191L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,075.00
RC217191L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged 10 ug
$1,075.00
RC217191L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,075.00
RC217191L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1, mGFP tagged 10 ug
$1,075.00
RG217191 MAP4K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$975.00
SC110237 MAP4K5 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 10 ug
$776.00

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