DCAMKL1 (DCLK1) (NM_004734) Human Tagged ORF Clone
- TrueORF®
SKU
RG217050
DCLK1 (tGFP-tagged) - Human doublecortin-like kinase 1 (DCLK1), transcript variant 1
$1,201.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DCAMKL1 |
| Synonyms | CL1; CLICK1; DCAMKL1; DCDC3A; DCLK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004734 |
| ORF Size | 2187 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004734.5 |
| RefSeq Size | 5703 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 9201 |
| UniProt ID | O15075 |
| Cytogenetics | 13q13.3 |
| Domains | DCX, pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.provided by RefSeq, Sep 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004734" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217050 | DCLK1 (Myc-DDK-tagged)-Human doublecortin-like kinase 1 (DCLK1), transcript variant 1 | 10 ug |
$1,001.00
|
|
| RC217050L1 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,301.00
|
|
| RC217050L2 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, mGFP tagged | 10 ug |
$1,301.00
|
|
| RC217050L3 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,301.00
|
|
| RC217050L4 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, mGFP tagged | 10 ug |
$1,301.00
|
|
| SC314561 | DCLK1 (untagged)-Human doublecortin-like kinase 1 (DCLK1), transcript variant 1 | 10 ug |
$1,002.00
|
|
Citations
Doublecortin-Like Kinase 1 (DCLK1) Is a Novel NOTCH Pathway Signaling Regulator in Head and Neck Squamous Cell Carcinoma
Broner, Trujillo, Korzinkin et al
Front Oncol (2021) 11, 677051 DOI: 10.3389/fonc.2021.677051
Broner, Trujillo, Korzinkin et al
Front Oncol (2021) 11, 677051 DOI: 10.3389/fonc.2021.677051
Cited by 25
AI Summary: In this study, OriGene products—including DCLK1 siRNA (SR321518) and DCLK1 overexpression plasmid (RC221518)—were used to manipulate DCLK1 levels in head and neck squamous cell carcinoma cell lines, enabling the authors to demonstrate DCLK1's regulatory role in NOTCH pathway signaling.
Application of RNA-sequencing to identify transcriptome modification by DCLK1 in colorectal cancer cells
Li, Mei, Commey
Cancer Gene Ther. (2019)
Li, Mei, Commey
Cancer Gene Ther. (2019)
Doublecotin-Like Kinase 1 Increases Chemoresistance of Colorectal Cancer Cells through the Anti-Apoptosis Pathway
Li, Jones, Mei
J Stem Cell Res Ther (2019) 9 (3) DOI: 10.4172/2157-7633.1000447
Li, Jones, Mei
J Stem Cell Res Ther (2019) 9 (3) DOI: 10.4172/2157-7633.1000447
Doublecotin-like kinase 1 increases chemoresistance of colorectal cancer cells through the anti-apoptosis pathway
Li, Jones, Mei
J Stem Cell Res Ther (2019) 9 (3) DOI: 10.4172/2157-7633.1000447
Li, Jones, Mei
J Stem Cell Res Ther (2019) 9 (3) DOI: 10.4172/2157-7633.1000447
Dominant Expression of DCLK1 in Human Pancreatic Cancer Stem Cells Accelerates Tumor Invasion and Metastasis
Ito, Tanaka, Akiyama et al
PLoS One (2016) 11 (1), e0146564 DOI: 10.1371/journal.pone.0146564
Ito, Tanaka, Akiyama et al
PLoS One (2016) 11 (1), e0146564 DOI: 10.1371/journal.pone.0146564
Cited by 84
AI Summary: OriGene products contributed to this study by providing the DCLK1 cDNA (RC211445) for overexpression experiments and DCLK1 shRNA (TF312594) for gene knockdown, enabling the authors to investigate the role of DCLK1 in pancreatic cancer stem cell invasion and metastasis.
Small molecule kinase inhibitor LRRK2-IN-1 demonstrates potent activity against colorectal and pancreatic cancer through inhibition of doublecortin-like kinase 1
Weygant, Qu, Berry et al
Mol. Cancer (2014) 13 (1), 103 DOI: 10.1186/1476-4598-13-103
Weygant, Qu, Berry et al
Mol. Cancer (2014) 13 (1), 103 DOI: 10.1186/1476-4598-13-103
Cited by 117
AI Summary: OriGene’s human DCLK1 isoform short-α cDNA tagged with turboGFP was used to generate a kinase-dead DCLK1 mutant, enabling the study of DCLK1 kinase activity’s role in cancer cell behavior in this research.
Snippet: Human DCLK1 isoform short-α cDNA tagged with turboGFP (tGFP) (Origene) was used to create a kinase-dead mutant, in which the lysine at residue 419 was substituted with an arginine by site-directed mutagenesis using the QuickChange II mutagenesis kit (Agilent Technologies)
Showing 1-6 of 6 papers.
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