DCAMKL1 (DCLK1) (NM_004734) Human Tagged ORF Clone
$1,001.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DCAMKL1 |
| Synonyms | CL1; CLICK1; DCAMKL1; DCDC3A; DCLK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004734 |
| ORF Size | 2187 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004734.5 |
| RefSeq Size | 5703 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 9201 |
| UniProt ID | O15075 |
| Cytogenetics | 13q13.3 |
| Domains | DCX, pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 80.9 kDa |
| Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.provided by RefSeq, Sep 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004734" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217050L1 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,301.00
|
|
| RC217050L2 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, mGFP tagged | 10 ug |
$1,301.00
|
|
| RC217050L3 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,301.00
|
|
| RC217050L4 | Lenti ORF clone of Human doublecortin-like kinase 1 (DCLK1), transcript variant 1, mGFP tagged | 10 ug |
$1,301.00
|
|
| RG217050 | DCLK1 (tGFP-tagged) - Human doublecortin-like kinase 1 (DCLK1), transcript variant 1 | 10 ug |
$1,201.00
|
|
| SC314561 | DCLK1 (untagged)-Human doublecortin-like kinase 1 (DCLK1), transcript variant 1 | 10 ug |
$1,002.00
|
|
Citations
DCLK1 induces a pro-tumorigenic phenotype to drive gastric cancer progression
Afshar-Sterle, Carli, O'Keefe et al
Sci Signal (2024) 17 (854), eabq4888 DOI: 10.1126/scisignal.abq4888
Afshar-Sterle, Carli, O'Keefe et al
Sci Signal (2024) 17 (854), eabq4888 DOI: 10.1126/scisignal.abq4888
Cited by 7
AI Summary: OriGene’s DCLK1 isoform 1 plasmid (catalog no. RC217050) was used as the template for PCR amplification and cloning of DCLK1 constructs, enabling the study of DCLK1’s role in gastric cancer progression.
Snippet: ing the DCLK1 isoform 1 (accession no. NM_004734) was PCR amplified from a plasmid purchased from Origene (Origene Technologies, Rockville, MD, USA;catalog no. RC217050) using forward primer 5′ agc aag ctt gcc acc atg tcc ttc ggc aga gac atg gag 3′ and reverse primer 5′ acg gga tcc cta cat cct ggt tgc gtc ttc gtc 3′ and subcloned into pcDNA3 using HindIII and BamH
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Carli, Afshar-Sterle, Rai et al
Proteomics (2021)
Carli, Afshar-Sterle, Rai et al
Proteomics (2021)
Cited by 27
AI Summary: OriGene’s DCLK1 isoform 1 plasmid (RC217050) was used as the source for DCLK1 gene amplification and overexpression in gastric cancer cells, enabling the study of DCLK1’s role in reprogramming small extracellular vesicles toward a migratory phenotype.
Snippet: The DCLK1isoform 1 (accession NM_004734) was PCR amplified from plasmid RC217050 (Origene) using forward primer 5' agc aag ctt gcc acc atg tcc ttc ggc aga gac atg gag 3'and reverse primer 5' acg gga tcc cta cat cct ggt tgc gtc ttc gtc 3' and subcloned into pcDNA3;- The DCLK1isoform 1 (accession NM_004734) was PCR amplified from plasmid RC217050 (Origene) using forward primer 5' agc aag ctt gcc acc atg tcc ttc ggc aga gac atg gag 3'and reverse primer 5' acg gga tcc cta cat cct ggt tgc gtc ttc gtc 3' and subcloned into pcDNA3
New age-related hearing loss candidate genes in humans: an ongoing challenge
Di Stazio, Morgan, Brumat et al
Gene (2020) 742, 144561 DOI: 10.1016/j.gene.2020.144561
Di Stazio, Morgan, Brumat et al
Gene (2020) 742, 144561 DOI: 10.1016/j.gene.2020.144561
Cited by 15
AI Summary: OriGene’s pCMV6-Entry vectors containing wild-type and mutant cDNAs of candidate genes (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3, and Strn) were used for transfection and protein expression studies to functionally assess gene variants associated with age-related hearing loss in this paper.
Snippet: The WT and human mutant genes of interest (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3 and Strn) cDNAs were cloned into a pCMV6-Entry vector (by Origene, Rockville, MD), Myc tagged, respectively. Vectors were purified through the PureLink HiPure Plasmid Maxiprep kit (Invitrogen) for transfection and protein expression
Doublecortin like kinase 1 regulates α-Synuclein levels and toxicity
Vázquez-Vélez, Gonzales, Revelli et al
J. Neurosci. (2019) 40 (2), 459-477 DOI: 10.1523/JNEUROSCI.1076-19.2019
Vázquez-Vélez, Gonzales, Revelli et al
J. Neurosci. (2019) 40 (2), 459-477 DOI: 10.1523/JNEUROSCI.1076-19.2019
AI Summary: OriGene’s human DCLK1 cDNA (RC217050) was used as the template to generate full-length and domain-specific DCLK1 constructs for investigating the role of DCLK1 in regulating α-Synuclein levels and toxicity.
Snippet: …Neurosci 2019;codes a 3’ 3X-Flag tag (Klisch et al., 2017). We obtained human DCLK1 cDNA 431 (RC217050, OriGene), and PCR amplified the full length DCLK1 (1-2188bp), PEST-Kinase domain 432 (862-1941bp), and Doublecortin domains (169-807bp) from the original vector. …
Pancreatic Neuroendocrine Tumors and EMT Behavior are Driven by the CSC Marker DCLK1
Ikezono, Akiba, Abe et al
Mol. Cancer Res. (2017)
Ikezono, Akiba, Abe et al
Mol. Cancer Res. (2017)
Showing 1-5 of 5 papers.
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