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SLC26A5 (NM_198999) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG216363
SLC26A5 (tGFP-tagged) - Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $881.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC26A5
Synonyms DFNB61; PRES
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG216363 representing NM_198999
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCATGCTGAAGAAAATGAAATCCTTGCAGCAACCCAGAGGTACTATGTGGAAAGGCCTATCTTTA
GTCATCCGGTCCTCCAGGAAAGACTACACACAAAGGACAAGGTTCCTGATTCCATTGCGGATAAGCTGAA
ACAGGCATTCACATGTACTCCTAAAAAAATAAGAAATATCATTTATATGTTCCTACCCATAACTAAATGG
CTGCCAGCATACAAATTCAAGGAATATGTGTTGGGTGACTTGGTCTCAGGCATAAGCACAGGGGTGCTTC
AGCTTCCTCAAGGCTTAGCCTTTGCAATGCTGGCAGCTGTGCCTCCAATATTTGGCCTGTACTCTTCATT
TTACCCTGTTATCATGTATTGTTTTCTTGGAACCTCCAGACACATATCCATAGGTCCTTTTGCTGTTATT
AGCCTGATGATTGGTGGTGTAGCTGTTCGATTAGTACCAGATGATATAGTCATTCCAGGAGGAGTAAATG
CAACCAATGGCACAGAGGCCAGAGATGCCTTGAGAGTGAAAGTCGCCATGTCTGTGACCTTACTTTCAGG
AATCATTCAGTTTTGCCTAGGTGTCTGTAGGTTTGGATTTGTGGCCATATATCTCACAGAGCCTCTGGTC
CGTGGGTTTACCACCGCAGCAGCTGTGCATGTCTTCACCTCCATGTTAAAATATCTGTTTGGAGTTAAAA
CAAAGCGGTACAGTGGAATCTTTTCCGTGGTGTATAGTACAGTTGCTGTGTTGCAGAATGTTAAAAACCT
CAACGTGTGTTCCCTAGGCGTCGGGCTGATGGTTTTTGGTTTGCTGTTGGGTGGCAAGGAGTTTAATGAG
AGATTTAAAGAGAAATTGCCGGCGCCTATTCCTTTAGAGTTCTTTGCGGTCGTAATGGGAACTGGCATTT
CAGCTGGGTTTAACTTGAAAGAATCATACAATGTGGATGTCGTTGGAACACTTCCTCTAGGGCTGCTACC
TCCAGCCAATCCGGACACCAGCCTCTTCCACCTTGTGTACGTAGATGCCATTGCCATAGCCATCGTTGGA
TTTTCAGTGACCATCTCCATGGCCAAGACCTTAGCAAATAAACATGGCTACCAGGTTGACGGCAATCAGG
AGCTCATTGCCCTGGGACTGTGCAATTCCATTGGCTCACTCTTCCAGACCTTTTCAATTTCATGCTCCTT
GTCTCGAAGCCTTGTTCAGGAGGGAACCGGTGGGAAGACACAGCTTGCAGGTTGTTTGGCCTCATTAATG
ATTCTGCTGGTCATATTAGCAACTGGATTCCTCTTTGAATCATTGCCCCAGGCTGTGCTGTCGGCCATTG
TGATTGTCAACCTGAAGGGAATGTTTATGCAGTTCTCAGATCTCCCCTTTTTCTGGAGAACCAGCAAAAT
AGAGCTGACCATCTGGCTTACCACTTTTGTGTCCTCCTTGTTCCTGGGATTGGACTATGGTTTGATCACT
GCTGTGATCATTGCTCTGCTGACTGTGATTTACAGAACACAGAGTCCAAGCTACAAAGTCCTTGGAAAGC
TTCCTGAAACTGATGTGTATATTGATATAGACGCATATGAGGAGGTGAAAGAAATTCCTGGAATAAAAAT
ATTTCAAATAAATGCACCAATTTACTATGCAAATAGCGACTTGTATAGCAATGCATTAAAACGAAAGACT
GGAGTGAACCCAGCAGTCATCATGGGAGCAAGGAGAAAGGCCATGCGGAAGTACGCTAAGGAAGTCGGAA
ATGCAAATATGGCCAACGCAACTGTTGTCAAAGCAGATGCAGAAGTAGATGGAGAGGATGCTACCAAGCC
TGAAGAAGAGGATGGTGAAGTAAAATATCCCCCAATAGTGATCAAAAGCACATTTCCTGAGGAAATGCAA
AGATTTATGCCCCCAGGGGATAACGTCCACACTGTCATTTTGGATTTCACTCAAGTCAATTTTATTGATT
CTGTTGGAGTGAAAACTCTGGCAGGGATTGTAAAAGAATATGGAGACGTCGGTATATATGTATACTTAGC
AGGATGCAGTGCACAAGTTGTGAATGACCTCACTCGGAATAGATTTTTTGAAAATCCTGCCCTATGGGAG
CTGCTGTTCCACAGCATTCATGATGCAGTTTTAGGCAGCCAACTTAGAGAGGCACTTGCTGAACAGGAAG
CCTCGGCTCCCCCTTCCCAGGAGGACTTGGAGCCCAATGCCACTCCTGCCACTCCTGAGGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG216363 representing NM_198999
Red=Cloning site Green=Tags(s)

MDHAEENEILAATQRYYVERPIFSHPVLQERLHTKDKVPDSIADKLKQAFTCTPKKIRNIIYMFLPITKW
LPAYKFKEYVLGDLVSGISTGVLQLPQGLAFAMLAAVPPIFGLYSSFYPVIMYCFLGTSRHISIGPFAVI
SLMIGGVAVRLVPDDIVIPGGVNATNGTEARDALRVKVAMSVTLLSGIIQFCLGVCRFGFVAIYLTEPLV
RGFTTAAAVHVFTSMLKYLFGVKTKRYSGIFSVVYSTVAVLQNVKNLNVCSLGVGLMVFGLLLGGKEFNE
RFKEKLPAPIPLEFFAVVMGTGISAGFNLKESYNVDVVGTLPLGLLPPANPDTSLFHLVYVDAIAIAIVG
FSVTISMAKTLANKHGYQVDGNQELIALGLCNSIGSLFQTFSISCSLSRSLVQEGTGGKTQLAGCLASLM
ILLVILATGFLFESLPQAVLSAIVIVNLKGMFMQFSDLPFFWRTSKIELTIWLTTFVSSLFLGLDYGLIT
AVIIALLTVIYRTQSPSYKVLGKLPETDVYIDIDAYEEVKEIPGIKIFQINAPIYYANSDLYSNALKRKT
GVNPAVIMGARRKAMRKYAKEVGNANMANATVVKADAEVDGEDATKPEEEDGEVKYPPIVIKSTFPEEMQ
RFMPPGDNVHTVILDFTQVNFIDSVGVKTLAGIVKEYGDVGIYVYLAGCSAQVVNDLTRNRFFENPALWE
LLFHSIHDAVLGSQLREALAEQEASAPPSQEDLEPNATPATPEA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198999
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198999.3
RefSeq Size 2671 bp
RefSeq ORF 2235 bp
Locus ID 375611
UniProt ID P58743
Cytogenetics 7q22.1
Protein Families Transmembrane
Summary This gene encodes a member of the SLC26A/SulP transporter family. The protein functions as a molecular motor in motile outer hair cells (OHCs) of the cochlea, inducing changes in cell length that act to amplify sound levels. The transmembrane protein is an incomplete anion transporter, and does not allow anions to cross the cell membrane but instead undergoes a conformational change in response to changes in intracellular Cl- levels that results in a change in cell length. The protein functions at microsecond rates, which is several orders of magnitude faster than conventional molecular motor proteins. Mutations in this gene are potential candidates for causing neurosensory deafness. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Nov 2009
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"NM_198999" in other vectors (6)
SKU Description Size Price
RC216363 SLC26A5 (Myc-DDK-tagged)-Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a 10 ug
$681.00
RC216363L1 Lenti ORF clone of Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a, Myc-DDK-tagged 10 ug
$981.00
RC216363L2 Lenti ORF clone of Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a, mGFP tagged 10 ug
$981.00
RC216363L3 Lenti ORF clone of Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a, Myc-DDK-tagged 10 ug
$981.00
RC216363L4 Lenti ORF clone of Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a, mGFP tagged 10 ug
$981.00
SC307845 SLC26A5 (untagged)-Human solute carrier family 26, member 5 (prestin) (SLC26A5), transcript variant a 10 ug
$682.00

Plasmid Map for RG216363

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG216363
Circular map for RG216363

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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