GRHL2 (NM_024915) Human Tagged ORF Clone
$1,072.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GRHL2 |
| Synonyms | BOM; DFNA28; ECTDS; PPCD4; TFCP2L3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_024915 |
| ORF Size | 1875 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024915.4 |
| RefSeq Size | 5231 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 79977 |
| UniProt ID | Q6ISB3 |
| Cytogenetics | 8q22.3 |
| Protein Families | Transcription Factors |
| Summary | The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).provided by RefSeq, Mar 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024915" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214498 | GRHL2 (Myc-DDK-tagged)-Human grainyhead-like 2 (Drosophila) (GRHL2) | 10 ug |
$872.00
|
|
| RC214498L1 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC214498L2 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), mGFP tagged | 10 ug |
$1,172.00
|
|
| RC214498L3 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC214498L4 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), mGFP tagged | 10 ug |
$1,172.00
|
|
| SC305189 | GRHL2 (untagged)-Human grainyhead-like 2 (Drosophila) (GRHL2) | 10 ug |
$874.00
|
|
Citations
Elevated GRHL2 Imparts Plasticity in ER-Positive Breast Cancer Cells
Zheng, Allen, Liu et al
Cancers (Basel) (2024) 16 (16) DOI: 10.3390/cancers16162906
Zheng, Allen, Liu et al
Cancers (Basel) (2024) 16 (16) DOI: 10.3390/cancers16162906
Cited by 4
AI Summary: OriGene's GRHL2-GFP plasmid (#RG214498) was used to generate inducible breast cancer cell lines, enabling the study of how elevated GRHL2 affects cell plasticity and progression in ER-positive breast cancer.
Snippet: capable of expressing GRHL2-GFP upon exposure to doxycycline hyclate (Dox;Sigma-Aldrich, Burlington, MA, USA, #D9891) was engineered. A tet-inducible construct was created using a GRHL2-GFP plasmid (Origene, Rockville, MD, USA, #RG214498) subcloned into a pUHD10-3 backbone downstream of the tet response element [27,28]. GRHL2-GFP-inducible MCF7 cell clones were generated by stable co-transfection
GRHL2 Enhances Phosphorylated Estrogen Receptor (ER) Chromatin Binding and Regulates ER-Mediated Transcriptional Activation and Repression
Reese, Helzer, Allen et al
Mol Cell Biol (2022) 42 (10), e0019122 DOI: 10.1128/mcb.00191-22
Reese, Helzer, Allen et al
Mol Cell Biol (2022) 42 (10), e0019122 DOI: 10.1128/mcb.00191-22
Cited by 16
Showing 1-2 of 2 papers.
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