GRHL2 (NM_024915) Human Tagged ORF Clone
$872.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GRHL2 |
| Synonyms | BOM; DFNA28; ECTDS; PPCD4; TFCP2L3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_024915 |
| ORF Size | 1875 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024915.4 |
| RefSeq Size | 4793 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 79977 |
| UniProt ID | Q6ISB3 |
| Cytogenetics | 8q22.3 |
| Protein Families | Transcription Factors |
| MW | 70.9 kDa |
| Summary | The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).provided by RefSeq, Mar 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024915" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214498L1 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC214498L2 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), mGFP tagged | 10 ug |
$1,172.00
|
|
| RC214498L3 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC214498L4 | Lenti ORF clone of Human grainyhead-like 2 (Drosophila) (GRHL2), mGFP tagged | 10 ug |
$1,172.00
|
|
| RG214498 | GRHL2 (tGFP-tagged) - Human grainyhead-like 2 (Drosophila) (GRHL2) | 10 ug |
$1,072.00
|
|
| SC305189 | GRHL2 (untagged)-Human grainyhead-like 2 (Drosophila) (GRHL2) | 10 ug |
$874.00
|
|
Citations
VULCAN integrates ChIP-seq with patient-derived co-expression networks to identify GRHL2 as a key co-regulator of ERa at enhancers in breast cancer
Holding, Giorgi, Donnelly et al
Genome Biol (2019) 20 (1), 91 DOI: 10.1186/s13059-019-1698-z
Holding, Giorgi, Donnelly et al
Genome Biol (2019) 20 (1), 91 DOI: 10.1186/s13059-019-1698-z
Cited by 47
AI Summary: In this study, OriGene’s GRHL2 shRNA (OriGene, TL313902) was used to knock down GRHL2 expression in breast cancer cells, enabling the authors to demonstrate GRHL2’s functional role as a co-regulator of estrogen receptor activity.
VULCAN: Network Analysis of ER-α activation reveals reprogramming of GRHL2
Holding, Giorgi, Donnelly et al
bioRxiv (2018)
Holding, Giorgi, Donnelly et al
bioRxiv (2018)
Snippet: MCF7 cells were transfected with Smart Pool siRNA (Dharmacon, L-014515-02), siControl, GRHL2 overexpression vector (Origene, RC214498) or empty control vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol in 6 well format. … Knockdown efficiency was ~75% and the GRHL2 overexpression vector led a 730- fold increase in expression over control plasmid. 1 ug of purified RNA was reverse transcribed with Superscript III reverse transcriptase (Thermo Fisher Scientific, 18080085) using random primers (Promega, C1181) according to manufacturer instructions. …
Suppression of the grainyhead transcription factor 2 gene (GRHL2) inhibits the proliferation, migration, invasion and mediates cell cycle arrest of ovarian cancer cells
Faddaoui, Sheta, Bachvarova et al
Cell Cycle (2017) 16 (7), 693-706 DOI: 10.1080/15384101.2017.1295181
Faddaoui, Sheta, Bachvarova et al
Cell Cycle (2017) 16 (7), 693-706 DOI: 10.1080/15384101.2017.1295181
Cited by 34
VULCAN integrates ChIP-seq with patient-derived co-expression networks to identify GRHL2 as a key co-regulator of ERa at enhancers in breast cancer
Holding, Giorgi, Donnelly et al
bioRxiv (2019)
Holding, Giorgi, Donnelly et al
bioRxiv (2019)
Snippet: ment and GRHL2’s own ability to regulate EMT, the discovery that ER recruits GRHL2 leading to the altered eRNA production is an important step in enhancing our understanding of breast cancer and tumorigenesis. Methods VULCAN An implementation of VULCAN in R is available on Bioconductor.org [https://bioconductor.org/packages/release/bioc/html/vulcan.html], and the scripts to replicate our analys;;MCF7 cells were transfected with Smart Pool siRNA (Dharmacon, L01451502), siControl, GRHL2 overexpression vector (Origene, RC214498), GRHL2 Δ425437 (Origene), or empty control vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol in 6well format
Showing 1-4 of 4 papers.
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