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ZCWPW1 (NM_017984) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG214455
ZCWPW1 (tGFP-tagged) - Human zinc finger, CW type with PWWP domain 1 (ZCWPW1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $803.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZCWPW1
Synonyms ZCW1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG214455 representing NM_017984
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGACAACGTTGCAGAATAAAGAAGAATGTGGAAAGGGACCAAAGAGAATCTTTGCCCCACCTGCAC
AAAAATCTTACAGCCTGTTACCTTGTAGCCCTAACTCCCCTAAGGAGGAGACCCCGGGGATCAGTTCCCC
AGAGACAGAGGCCAGGATAAGCCTGCCAAAGGCCAGTTTAAAGAAGAAAGAGGAAAAAGCAACCATGAAG
AATGTTCCAAGCAGGGAACAGGAGAAAAAAAGAAAGGCACAAATCAACAAGCAAGCAGAGAAGAAAGAAA
AGGAAAAATCAAGTCTTACCAATGCAGAATTTGAGGAGATTGTCCAGATTGTTCTGCAGAAGTCCCTTCA
GGAGTGCTTGGGGATGGGATCTGGCCTTGATTTTGCAGAGACTTCTTGTGCCCAGCCCGTAGTATCTACC
CAATCAGACAAGGAGCCAGGAATTACTGCTTCTGCTACTGATACTGATAATGCTAATGGAGAGGAGGTAC
CACATACTCAAGAGATTTCAGTGTCTTGGGAAGGTGAAGCTGCCCCTGAGATAAGGACATCTAAGTTAGG
CCAGCCAGATCCTGCACCCTCTAAGAAGAAATCCAATAGACTCACCTTAAGCAAAAGAAAGAAGGAAGCT
CATGAGAAGGTGGAGAAAACTCAAGGTGGACATGAGCACAGACAGGAAGACCGACTAAAGAAAACAGTTC
AGGATCATTCTCAGATCAGGGACCAGCAAAAAGGAGAGATAAGTGGTTTTGGTCAATGTCTGGTCTGGGT
CCAGTGTTCCTTCCCAAACTGTGGGAAATGGAGGCGGCTGTGTGGGAACATTGACCCCTCAGTTCTCCCA
GATAATTGGTCCTGTGATCAGAACACAGATGTGCAGTATAATCGCTGTGATATTCCTGAGGAGACCTGGA
CAGGGCTTGAGAGTGATGTGGCCTATGCCTCCTACATCCCAGGATCCATCATCTGGGCCAAGCAATACGG
TTACCCCTGGTGGCCAGGCATGATAGAATCTGATCCTGACTTAGGGGAATATTTTCTTTTTACTTCCCAT
CTTGATTCCCTGCCGTCTAAGTACCATGTGACGTTTTTTGGAGAAACAGTTTCTCGTGCATGGATCCCAG
TCAACATGCTAAAGAACTTCCAGGAGCTGTCCCTGGAGCTATCAGTCATGAAAAAGCGCAGAAATGACTG
CAGCCAGAAACTGGGGGTGGCCCTGATGATGGCTCAAGAGGCAGAACAGATCAGCATTCAGGAACGGGTT
AACTTGTTTGGTTTCTGGAGCCGATTCAACGGATCTAACAGTAATGGGGAAAGAAAAGACTTACAGCTCT
CTGGTTTGAACAGCCCAGGATCCTGCTTAGAGAAAAAGGAGAAAGAGGAAGAGTTGGAAAAGGAGGAAGG
AGAGAAAACAGACCCAATTTTGCCCATTCGTAAGCGAGTCAAAATACAGACCCAAAAAACCAAGCCAAGA
GGGCTTGGGGGTGATGCAGGCACAGCAGATGGCCGAGGCAGGACACTGCAGAGGAAGATAATGAAGAGAT
CTCTAGGCAGGAAATCCACAGCTCCTCCTGCACCCAGAATGGGAAGGAAAGAAGGCCAAGGGAATTCAGA
TTCTGACCAGCCAGGCCCTAAGAAAAAATTTAAAGCTCCCCAGAGCAAGGCCTTGGCAGCCAGCTTTTCA
GAGGGAAAAGAAGTTAGAACAGTGCCAAAGAACCTGGGCCTATCAGCGTGTAAGGGGGCCTGCCCCTCAT
CTGCGAAAGAAGAGCCCAGACACCGGGAACCCCTGACCCAGGAGGCTGGAAGTGTCCCCCTTGAGGACGA
AGCCTCCAGTGACCTGGACCTGGAGCAACTCATGGAAGATGTTGGGAGAGAGCTGGGGCAGAGCGGGGAG
CTGCAGCACAGCAACAGTGATGGCGAGGACTTCCCCGTGGCGCTGTTTGGGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG214455 representing NM_017984
Red=Cloning site Green=Tags(s)

MMTTLQNKEECGKGPKRIFAPPAQKSYSLLPCSPNSPKEETPGISSPETEARISLPKASLKKKEEKATMK
NVPSREQEKKRKAQINKQAEKKEKEKSSLTNAEFEEIVQIVLQKSLQECLGMGSGLDFAETSCAQPVVST
QSDKEPGITASATDTDNANGEEVPHTQEISVSWEGEAAPEIRTSKLGQPDPAPSKKKSNRLTLSKRKKEA
HEKVEKTQGGHEHRQEDRLKKTVQDHSQIRDQQKGEISGFGQCLVWVQCSFPNCGKWRRLCGNIDPSVLP
DNWSCDQNTDVQYNRCDIPEETWTGLESDVAYASYIPGSIIWAKQYGYPWWPGMIESDPDLGEYFLFTSH
LDSLPSKYHVTFFGETVSRAWIPVNMLKNFQELSLELSVMKKRRNDCSQKLGVALMMAQEAEQISIQERV
NLFGFWSRFNGSNSNGERKDLQLSGLNSPGSCLEKKEKEEELEKEEGEKTDPILPIRKRVKIQTQKTKPR
GLGGDAGTADGRGRTLQRKIMKRSLGRKSTAPPAPRMGRKEGQGNSDSDQPGPKKKFKAPQSKALAASFS
EGKEVRTVPKNLGLSACKGACPSSAKEEPRHREPLTQEAGSVPLEDEASSDLDLEQLMEDVGRELGQSGE
LQHSNSDGEDFPVALFGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017984
ORF Size 1944 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017984.5
RefSeq Size 2356 bp
RefSeq ORF 1947 bp
Locus ID 55063
UniProt ID Q9H0M4
Cytogenetics 7q22.1
Domains PWWP
Summary Dual histone methylation reader specific for PRDM9-catalyzed histone marks (H3K4me3 and H3K36me3) (PubMed:32744506, PubMed:20826339). Facilitates the repair of PRDM9-induced meiotic double-strand breaks (DSBs) (By similarity). Essential for male fertility and spermatogenesis (By similarity). Required for meiosis prophase I progression in male but not in female germ cells (By similarity).UniProtKB/Swiss-Prot Function
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"NM_017984" in other vectors (4)
SKU Description Size Price
RC214455 ZCWPW1 (Myc-DDK-tagged)-Human zinc finger, CW type with PWWP domain 1 (ZCWPW1) 10 ug
$603.00
RC214455L3 Lenti ORF clone of Human zinc finger, CW type with PWWP domain 1 (ZCWPW1), Myc-DDK-tagged 10 ug
$903.00
RC214455L4 Lenti ORF clone of Human zinc finger, CW type with PWWP domain 1 (ZCWPW1), mGFP tagged 10 ug
$903.00
SC318109 ZCWPW1 (untagged)-Human zinc finger, CW type with PWWP domain 1 (ZCWPW1) 10 ug
$664.00

Plasmid Map for RG214455

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG214455
Circular map for RG214455

file-alt Citations

ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair
Wells, Bitoun, Moralli et al
Elife (2020) 9 DOI: 10.7554/eLife.53392
AI Summary: In this study, OriGene's Zcwpw1 cDNA clone (MR209824) was used to express ZCWPW1 protein in human cells, enabling the authors to investigate its recruitment to PRDM9 binding sites and its essential role in meiotic double-strand break repair.
FigureFigureFigure
Showing 1-1 of 1 papers.
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