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PAPOLG (NM_022894) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG213918
PAPOLG (tGFP-tagged) - Human poly(A) polymerase gamma (PAPOLG)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $874.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAPOLG
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG213918 representing NM_022894
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGAGATGTCTGCAAACACCGTGCTGGACAGCCAGCGTCAACAAAAGCATTATGGAATTACCTCCC
CAATTAGTTTGGCATCTCCTAAAGAAATTGATCATATTTACACACAGAAATTAATTGACGCCATGAAACC
ATTTGGAGTGTTTGAAGATGAGGAAGAATTGAACCACAGGCTGGTGGTTCTTGGTAAATTGAACAATTTA
GTAAAAGAATGGATTTCTGATGTCAGCGAGAGTAAGAACCTCCCACCTTCTGTTGTGGCTACTGTTGGTG
GTAAAATTTTCACATTTGGATCCTATAGGCTTGGAGTACACACCAAAGGAGCTGACATTGATGCACTTTG
TGTAGCTCCAAGACATGTGGAAAGATCTGATTTTTTTCAGTCTTTTTTTGAAAAATTGAAACATCAAGAT
GGCATTAGAAACTTAAGAGCTGTAGAAGATGCCTTTGTACCTGTTATAAAATTTGAATTTGATGGTATTG
AAATTGATCTAGTCTTTGCAAGACTGGCAATACAAACCATATCAGATAATTTAGATCTAAGAGACGACTC
TCGCCTGAGAAGCCTTGATATAAGGTGTATTCGCAGCTTAAATGGTTGTAGAGTTACTGATGAAATTTTG
CATTTAGTGCCAAATAAAGAAACTTTTAGACTCACCCTAAGAGCTGTCAAATTATGGGCAAAACGACGTG
GTATTTATTCCAACATGCTAGGATTCCTTGGTGGTGTCTCCTGGGCAATGCTAGTTGCAAGAACTTGCCA
ATTGTATCCAAATGCAGCAGCATCTACTTTAGTTCATAAGTTCTTTTTAGTTTTTTCCAAGTGGGAATGG
CCAAATCCTGTGCTGCTGAAGCAACCAGAAGAAAGCAATTTGAATTTGCCTGTCTGGGATCCTCGGGTAA
ATCCATCAGATAGGTATCATCTCATGCCCATAATCACCCCTGCCTACCCACAACAGAATTCTACGTATAA
TGTGTCCACATCAACTCGAACAGTAATGGTAGAAGAATTTAAACAAGGTCTTGCAGTCACAGATGAAATT
CTTCAAGGAAAGTCAGATTGGTCCAAACTACTTGAGCCACCGAATTTCTTTCAAAAGTATAGACATTATA
TAGTATTGACTGCCAGCGCATCAACAGAAGAAAACCATCTAGAGTGGGTTGGATTAGTAGAATCTAAAAT
CCGTGTACTTGTTGGAAACTTGGAACGGAATGAATTTATTACTCTTGCCCATGTGAATCCCCAGTCATTC
CCAGGGAATAAGGAACATCATAAAGACAACAATTACGTATCAATGTGGTTCCTTGGGATAATTTTTCGGA
GAGTAGAAAATGCAGAAAGTGTCAACATAGACTTGACATATGATATACAGTCATTTACTGATACAGTGTA
CAGACAGGCAAACAATATAAATATGCTAAAGGAGGGAATGAAAATTGAAGCAACTCATGTAAAGAAAAAA
CAACTTCACCACTACCTTCCTGCAGAAATTCTTCAAAAGAAGAAAAAGCAAAGTCTCTCTGATGTCAATC
GAAGCTCGGGCGGACTTCAATCCAAAAGATTGTCTCTGGATAGCAGTTGTCTGGATAGCTCCAGAGACAC
TGATAATGGAACACCTTTTAATTCTCCAGCGTCCAAGTCTGATAGCCCTTCTGTAGGAGAAACAGAAAGG
AATAGTGCTGAGCCTGCTGCTGTAATTGTGGAGAAGCCACTGAGTGTACCACCAGCCCAAGGACTTTCCA
TTCCAGTGATTGGCGCAAAAGTTGACTCTACAGTAAAAACTGTATCACCCCCCACTGTGTGTACCATTCC
TACCGTAGTAGGACGAAATGTCATTCCTAGAATCACAACACCTCACAACCCTGCCCAGGGACAACCGCAT
CTGAATGGAATGTCAAATATAACTAAGACTGTTACACCTAAGAGATCCCATTCCCCATCCATAGATGGGA
CTCCTAAGAGGTTGAAAGACGTAGAAAAGTTTATTCGACTTGAATCAACATTTAAGGACCCCCGCACTGC
TGAAGAAAGAAAAAGAAAATCAGTGGATGCCATTGGAGGAGAATCTATGCCTATTCCAACTATTGATACA
TCACGCAAAAAGAGACTACCCAGTAAAGAACTACCAGATTCATCATCTCCAGTTCCAGCAAACAACATCC
GTGTCATCAAAAATTCCATTCGACTGACCCTTAATCGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG213918 representing NM_022894
Red=Cloning site Green=Tags(s)

MKEMSANTVLDSQRQQKHYGITSPISLASPKEIDHIYTQKLIDAMKPFGVFEDEEELNHRLVVLGKLNNL
VKEWISDVSESKNLPPSVVATVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERSDFFQSFFEKLKHQD
GIRNLRAVEDAFVPVIKFEFDGIEIDLVFARLAIQTISDNLDLRDDSRLRSLDIRCIRSLNGCRVTDEIL
HLVPNKETFRLTLRAVKLWAKRRGIYSNMLGFLGGVSWAMLVARTCQLYPNAAASTLVHKFFLVFSKWEW
PNPVLLKQPEESNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSTSTRTVMVEEFKQGLAVTDEI
LQGKSDWSKLLEPPNFFQKYRHYIVLTASASTEENHLEWVGLVESKIRVLVGNLERNEFITLAHVNPQSF
PGNKEHHKDNNYVSMWFLGIIFRRVENAESVNIDLTYDIQSFTDTVYRQANNINMLKEGMKIEATHVKKK
QLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETER
NSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPH
LNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDT
SRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022894
ORF Size 2208 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022894.4
RefSeq Size 4252 bp
RefSeq ORF 2211 bp
Locus ID 64895
UniProt ID Q9BWT3
Cytogenetics 2p16.1
Domains NTP_transf_2, PAP_central, PAP_RNA-bind
Protein Families Transcription Factors
Protein Pathways RNA degradation
Summary This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. provided by RefSeq, Jul 2008
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"NM_022894" in other vectors (4)
SKU Description Size Price
RC213918 PAPOLG (Myc-DDK-tagged)-Human poly(A) polymerase gamma (PAPOLG) 10 ug
$674.00
RC213918L3 Lenti ORF clone of Human poly(A) polymerase gamma (PAPOLG), Myc-DDK-tagged 10 ug
$974.00
RC213918L4 Lenti ORF clone of Human poly(A) polymerase gamma (PAPOLG), mGFP tagged 10 ug
$974.00
SC310141 PAPOLG (untagged)-Human poly(A) polymerase gamma (PAPOLG) 10 ug
$742.00

Plasmid Map for RG213918

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG213918
Circular map for RG213918

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